J. Planar Chromatogr. 23, 332-334 (2010). TLC of fenvalerate, cypermethrin, and deltamethrin (as standards and extracted from minced visceral tissue) on silica gel with petroleum ether - diethyl ether 9:1 in a previously saturated TLC chamber. Detection by spraying successively with 10 % sodium hydroxide and then with 0.2 % p-benzoquinone in dimethyl sulfoxide. The LOD were approximately 0.25, 0.5, and 0.25 µg/zone for cypermethin, fenvalerate, and deltamethrin, respectively. Organochloro, organophosphorus, carbamate insecticides and pyrethroid insecticides without a cyanide group do not interfere.

J. of China Three Gorges Univ. Natural Sciences 33 (4), 92-95 (2011). TLC of extracts of Biyuan Pills on silica gel 1) for Magnolia liliiflora Desr. with dichloromethane - ethyl acetate 9:1, detection by spraying with 10 % sulfuric acid in ethanol and heating at 90 °C until the zones were detected; 2) for Xanthium sibiricum Patr., with chloroform - ethyl acetate - methanol - water - formic acid 3:10:2:2:2, detection by exposure to iodine vapor until the zones were detected; 3) for Lonicera japonica and Chrysanthemum indici flos, with toluene - ethyl acetate - formic acid - glacial acetic acid - water 1:15:1:1:2, detection under UV 365 nm.

Phytochem. Anal. 24, 1-24 (2013) The application of chemometrics in combination with chromatographic fingerprining was reviewed. The authors described how this combination allowed to find correlation between different variables such as molecular profile and genetic variability and their geographical origins and growing conditions.

Chinese J. of Anal. Instruments (Fenxi Yiqi) (1), 58-62 (2012). The knowledge of the time when a document was created is one of the primary coverages in expert testimony. Especially the identification of the stamping time of atomic seal ink on paper is quite different from that of handwriting and common seals on paper, due to the special feature of its particular ingredient. The coloring agents such as bronze red C and phthalocyanine blue are not the same on paper and on developed silica plates because they may be oxidized and permeate gradually into the deeper layer of the paper. Also permeation of adjuvant materials to the color zone may cause cross-linked reactions. Thus the character of the ink in the extracts from the paper may be changed as time passes. The samples of atomic seal ink on paper with different stamping times were extracted with the optimized solvent system N,N-dimethylformamide – n-butanol – dichloromethane 2:1:1. TLC of the extracts on silica gel with n-amyl alcohol – cyclohexane – ethyl acetate 2:1:1. Determination of the coloring agent in the extracts by densitometry at 470 nm. Calculation of the extraction rate R = Ct / C, where Ct is the scanning peak area of the extracts obtained at different extraction duration and C is that obtained at the time when extraction reaches its equilibrium stage. Determination of the stamping time of a sample by using the calibration curves of the extraction rate against the stamping time. Demonstration of the method by determination of ten varieties of atomic seal inks containing bronze red C and and stamping times ranging from 3 to 36 months.

J. of Qilu Med. & Pharm. 31 (2), 75-78 (2012). Pudilan Xiaojan oral liquid is a herbal TCM preparation effective in clearing heat and removing toxicity, because of its antiinflammatory activity it is used to cure swelling caused by furuncles, pharyngitis, lymphadenitis and tonsillitis. Dandelion is the key component drug of the preparation. Analysis of the components of dandelion in the preparation by TLC on silica gel with chloroform – methanol – formic acid 18:2:1, detection under UV 366 nm.

Chinese J. Ethnomed. Ethnopharm. (23), 70-71 (2011). Blood ginseng, the dried root of Indigofera stachyoides Lindl. is a traditional Chinese herbal crude drug with special effect in invigorating the circulation of blood, reducing phlegm, treating yin deficiency by reinforcing body fluid and nourishing the blood. It is used clinically to treat encephalalgia, woman’s abdominal pain, and metrorrhagia. For quality control 1) TLC of the extracts on silica gel with toluene – ethyl acetate – formic acid 6:3:1, detection in daylight; 2) determination of the moisture by drying in the oven; 3) determination of general ash content by firing, and of the acid insoluble ash content by acid pickling; 4) determination of ethanol extract by lixiviating with 50 % ethanol.

J. of China Pharm. 21 (12), 15-16 (2012). The leaf of Microcos paniculata L. is a popular medicinal herbal drug used for cardiovascular protection, blood fat adjusting, and anti-aging. Presentation of methods for identifying the drug by TLC and FTIR. TLC of the extracts of the drug on silica gel 1) for kaempferide, with toluene – ethyl acetate – formic acid 9:3:1, detection by spraying with 3 % aluminiumchloride in ethanol followed by heating at 105 ºC for 5 min and viewing at UV 366 nm; 2) for vitexin and isovitexin, developed with ethyl acetate – methanol – water 100:17:13, detection by spraying with 3 % aluminiumchloride in ethanol followed by heating at 105 ºC for 5 min and viewing at UV 366 nm. The methods have been applied to 15 batches of real life sample available from different places of origin, and proved that TLC procedures as well as FTIR are simple, sensitive, reproducible and robust and suitable for the identification of the herbal drug.

Chinese J. of Guide for Trad. Chinese Med. & Pharm. 18 (5), 80-81 (2012). Weijianning capsules, a herbal TCM preparation for tonifying spleen and invigorating the stomach, is prescribed clinically to treat superficial, erosive and atrophic gastritis. Presentation of a TLC method for the quality control of the preparations optimized by investigating the sample extraction procedure and the mobile phases employed. TLC of the extracts on silica gel 1) for Radix Scrophulariae with ethyl acetate – ethanol – water 20:5:3, detection by spraying with a solution of 3 g vanillin – 100 mL ethanol – 0.5 mL sulfuric acid and heating at 105 °C until the zones were clearly visualized, evaluation in daylight; 2) for Fructus Amomi with n-hexane - ethyl acetate 3:1, detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 °C until the zones were clearly visualized, evaluation in daylight. Identification by fingerprint comparison in parallel with the individual standard components.