Chemosphere. 87, 265-272 (2012). HPTLC of different dyes, namely Scarlet RR
(1), Rubine GFL (2), Brilliant Blue R (3), Navy Blue 2R (4) and Red HE3B (5) and ethyl acetate extracted metabolites (6) on silica gel with toluene - ethyl acetate - methanol 7:2:1. Quantitative determination by absorbance measurement at 280 nm at various time intervals (0-96 h). The hRF values of (1) to (5) were 90, 82, 79, 69 and 49, respectively. The hRF values of (6) were 86, 63, 56, 44, 34, 27, 18, 11 and 5. The method allowed to study the progressive decolorization and preferential degradation of the dyes within a mixture by Glandularia pulchella.

Chemosphere. 90, 2157-2171 (2013). HPTLC of glycerophospholipids in liver and brain of male Atlantic cod (Gadus morhua) on silica gel (washed with methyl acetate - isopropanol - chloroform - methanol - 0.25 % KCl 25:25:25:10:9 and activated at 120 ºC for 30 min) with hexane - diethylether - acetic acid 40:10:1. Detection by spraying with 0.1 % dichlorofluorescin in 98 % methanol and 0.001 % 3,5-di-tert-4butylhydroxytoluene (BHT). Lipid classes were futher analyzed by gas chromatography with a flame ionization detector. 

Anal. Bioanal. Chem. 413, 1941-1954 (2021). HPTLC of sulfoquinovosylmonoacylglycerol (1) and sulfoquinovosyldiacylglycerol (2) in a cyanobacterium (Arthrospira sp.), a microalgae preparation (Chlorella vulgaris) and selected leafy vegetables (spinach, basil) on silica gel with chloroform - methanol - acetic acid - water 425:75:50:18. Detection by dipping into  5 % copper sulfate in 8 % phosphoric acid, followed by heating at 160 ºC for 30 min.

Anal. Bioanal. Chem. 412, 6431-6448 (2020). Hydrophilic interaction high-performance
thin-layer chromatography (HI-HPTLC) of Stevia leaf extracts and 20 different food products on silica gel with acetonitrile - water 4:1. Detection by spraying with 2-naphthol sulfuric acid reagent (2 g in 180 mL ethanol plus dropwise 8 mL 50 % sulfuric acid) followed by heating at 160 ºC for 2 min, primuline reagent (0.1 g in 40 mL water plus 160 mL acetone), followed by documentation at 366 nm or anisaldehyde sulfuric acid reagent (1 mL 4-methoxybenzaldehyde, 8 mL sulfuric acid, 16 mL glacial acetic acid and 140 mL methanol), followed by heating at 110 ºC for 5 min. Quantitative determination by absorbance measurement at 500 nm after derivatization with the 2-naphthol sulfuric acid reagent and at 366/> 400 nm after derivatization with primuline reagent. Full scan mass spectra (m/z 100–1500) were recorded via heated electrospray ionization (HESI) in the positive and negative ionization mode. Bioprofiling and effect-directed detections were performed using a Gram-negative A. fischeri bioassay, Gram-positive B. subtilis bioassay, β-glucuronidase assay, tyrosinase assay, α-glucosidase assay, AChE assay and DPPH assay. 

Anal. Bioanal. Chem. 412, 6789-3809 (2020). HPTLC of Ginkgo biloba extracts on silica gel with ethyl acetate - acetic acid - formic acid - water 100:11:11:26 (1) or toluene - ethyl acetate - formic acid 7:3:1 (2). Detection under UV light at 366 nm or by spraying with  natural product reagent (NPR) and polyethylene glycol (PEG). The method allowed to distinguish between characteristic and uncharacteristic unfinished product samples based on the presence or absence of bands corresponding to caffeic acid, rutin, hyperoside, chlorogenic acid, and genistein standards.

Anal. Bioanal. Chem. 412, 4527-4536 (2020). HPTLC of 20 chemicals representative of migrants from plastic food contact materials on silica gel with chloroform - acetone - petroleum ether 11:5:5. Yeast estrogen screen was performed by spraying with yeast culture, followed by incubation at 30 ºC for 3 h. Detection by spraying with the indicator (2 mL 0.5 mg/mL 4-methylumbelliferyl-β-D-galactopyranoside-MUG in lacZ buffer), followed by incubation at 37 ºC for 20 min. Qualitative identification under UV light at 366 and 550 nm. The method was more sensitive than a microtiter plate YES (lyticase-YES). 

Anal. Bioanal. Chem. 413, 1321-1335 (2021). HPTLC of estrone (1), 17β-estradiol
(2), 17α-ethinylestradiol (3), 5α-dihydrotestosterone (4), and progesterone (5) in wastewater and surface water samples on silica gel with a mixture of dichloromethane, cyclohexane, and acetone in different proportions. Detection by spraying with 8 % sulfuric acid in ethanol, followed by heating at 105 ºC for 10 min. Qualitative identification under UV light at 310 nm. Yeast multi endocrine-effect screen was performed by spraying the HPTLC plates with a mixed suspension of genetically modified Arxula adeninivorans yeast strains, which contain either the human estrogen, androgen, or progesterone receptor. The HPTLC plates were incubated at 30 ºC for 18 h and at 100 % humidity. After incubation, densitometric evaluation at: 445/K460 nm, 475/K500 nm and 542/K560 nm to determine the fluorescence of the cyan fluorescent protein (CFP, gestagen), green fluorescent protein (GFP, androgen), and DsRed2 protein (estrogen), respectively. The hRF values for (1) to (5) were 21, 22, 29, 34 and 39, respectively.

Anal. Bioanal. Chem. 411, 725-734 (2019). HPTLC of bisphenol A (1) and its nine brominated analogs, namely monobromobisphenol A (2), 2,2’-dibromobisphenol A (3), tribromobisphenol A (4), tetrabromobisphenol A (TBBPA) (5), TBBPA mono(methyl ether) (6), TBBPA mono(allyl ether) (7), TBBPA mono(2,3-dibromopropylether) (8), TBBPA bis(allyl ether) (9), TBBPA bis(2,3-dibromopropyl ether) (10) in chicken samples on silica gel with n-hexane - ethyl acetate - dichloromethane - acetic acid 25:5:5:1. Detection at UV 254 nm. Further analysis by high-performance liquid chromatography-diode array detector/triple quadrupole mass spectrometry. The hRF values for (1) to (10) were 30, 32, 33, 45, 58, 68, 69, 69, 91 and 86, respectively.