Cumulative CAMAG Bibliography Service CCBS

Our CCBS database includes more than 11,000 abstracts of publications. Perform your own detailed search of TLC/HPTLC literature and find relevant information.

The Cumulative CAMAG Bibliography Service CCBS contains all abstracts of CBS issues beginning with CBS 51. The database is updated after the publication of every other CBS edition. Currently the Cumulative CAMAG Bibliography Service includes more than 11'000 abstracts of publications between 1983 and today. With the online version you can perform your own detailed TLC/HPTLC literature search:

  • Full text search: Enter a keyword, e.g. an author's name, a substance, a technique, a reagent or a term and see all related publications
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      127 028
      Estrogenic activity of food contact materials—evaluation of 20 chemicals using a yeast estrogen screen on HPTLC or 96-well plates
      A. J. BERGMANN*, E. SIMON, A. SCHIFFERLI, A. SCHOENBORN, E. VERMEIRSSEN (*Swiss Centre for Applied Ecotoxicology, Eawag, Überlandstrasse 133, 8600 Dübendorf, Switzerland, alanjames.bergmann@oekotoxzentrum.ch)

      Anal. Bioanal. Chem. 412, 4527-4536 (2020). HPTLC of 20 chemicals representative of migrants from plastic food contact materials on silica gel with chloroform - acetone - petroleum ether 11:5:5. Yeast estrogen screen was performed by spraying with yeast culture, followed by incubation at 30 ºC for 3 h. Detection by spraying with the indicator (2 mL 0.5 mg/mL 4-methylumbelliferyl-β-D-galactopyranoside-MUG in lacZ buffer), followed by incubation at 37 ºC for 20 min. Qualitative identification under UV light at 366 and 550 nm. The method was more sensitive than a microtiter plate YES (lyticase-YES). 

      Classification: 5b, 7
      127 005
      Utilization of a crown ether/amine‐type rotaxane as a probe for the versatile detection of anions and acids by Thin‐Layer Chromatography.
      S. MIYAGAWA, M. KIMURA, S. KAGAMI, T. KAWASAKI, Y. TOKUNAGA* (*Department of Materials Science and Engineering, University of Fukui, Bunkyo, Fukui, Japan; tokunaga@u-fukui.ac.jp)

      Chem. Asian J. 15(19), 3044-3049 (2020). The studied rotaxane combines a dibenzocrown of 8 ethers (DB24C8) with an axle chain (Ax) containing two amines, one of them in an aniline group, allowing stability of the rotaxane even when the other one is unprotonated. TLC on silica gel in 4 steps, with detection under UV light or after derivatization with phosphomolybdic acid in ethanol. (1) Before the synthesis of the rotaxane, unprotonated Ax was isolated by preparative TLC of the protonated Ax obtained by addition of HCl or toluenesulfonic acid (TsOH); the mobile phases were chloroform – methanol 10:1 and toluene – tetrahydrofurane 3:2, respectively. The isolated molecules were confirmed as totally unprotonated Ax by NMR, suggesting a complete loss of HCl and TsOH on the silica gel layer. (2) After synthesis, unprotonated rotaxane, pure vs. monoprotonated by the addition of 10 different acids (and purified by column chromatography CC), was applied on TLC plates and developed with dichloromethane – acetone – water 3:16:1; the hRF values were very different, depending on the counter-anions from the used acids. (3) The same behavior (except with sulfuric acid) was observed under the same conditions when CC was omitted (unprotonated rotaxane samples were mixed with each of the acids, or with two acids at the same time for acid-competitive TLC analysis). (4) When unprotonated rotaxane was applied under the same conditions as in step (3) with the sodium salts instead of the acids, the behavior was similar (except for the shapes of the spots, due to the salts in excess). The rotaxane can thus be used for the TLC separation and detection of sodium salts, by forming salts of protonated rotaxane with the anion afforded by these sodium salts. The rotaxane protonation seems to be promoted by the methanol of the spotting mixture; indeed, when step (3) was performed with the mobile phase chloroform – methanol 10:1, a second zone appeared because methanol formed a salt with the rotaxane (identified by NMR).

      Classification: 4e, 5a, 5b, 17a
      124 055
      Direct bioautography hyphenated to direct analysis in real time mass spectrometry: Chromatographic separation, bioassay and mass spectra, all in the same sample run
      T.T. HÄBE, M. JAMSHIDI-AIDJI, J. MACHO, Gertrud E. MORLOCK* (*Chair of Food Science, Institute of Nutritional Science, Interdisciplinary Research Center (IFZ), Justus Liebig Univ. Giessen, Heinrich-Buff-Ring 26-32, 35392, Giessen, Germany, Gertrud.Morlock@uni-giessen.de)

      J. of Chromatogr. A 1568, 188-196 (2018). Mass spectra by DART-MS were recorded directly in situ the bioautogram, immediately after direct bioautography (DB). This allowed to detect bioactive analytes within the bioautogram and discriminate microorganism cells and polar bioassay medium ingredients which could otherwise stress the MS system. DB-DART-MS was used for bioactive compounds in cosmetics using the Bacillus subtilis and Aliivibrio fischeri bioassays for detection of Gram-positive and Gram-negative antimicrobials. Planar yeast estrogen screen was used for detection of estrogen-effective compounds. HPTLC-DART-MS of parabens in hand creams either on silica gel with petroleum ether - glacial acetic acid 20:3 or on RP-18W with methanol - water 1:1. Detection under UV 254 and 366 nm. Bioassay by immersing the neutralized chromatograms into the bacterial suspensions.

      Keywords: densitometry HPTLC
      Classification: 4e, 5b
      100 020
      Chlorine-free mobile phase for determination of PAH in water extracts
      H. HEGEWALD (Lacrome Lda, Rua Cesar Batista 6 D, 7000 715 Evora, Portugal; lacrome@clix.pt)

      CBS 98, 9-11 (2007). Quantitative HPTLC of polycyclic aromatic hydrocarbons (PAH) from water samples, on caffeine-impregnated silica gel, with isopropyl acetate in a precooled (-20 °C, 30 min) twin-trough chamber without chamber saturation over 70 mm at -20°C. After application the dry plate was first equilibrated in the solvent-free trough for 10 min at -20 °C. Qualitative HPTLC at room temperature in the horizontal developing chamber with isopropyl acetate - n-hexane 3:1 over 50 mm. Detection by dipping in paraffin - toluene 1:1 (for fluorescence enhancement). Quantitative determination by fluorescence measurement at UV 366/>400 nm. Qualitative evaluation under UV 366 nm. The method is based on the German standard DIN 38407-7 for quantitative determination of 6 PAH but uses isopropyl acetate as a chlorine-free solvent instead of dichloromethane.

      Classification: 5b
      57 011
      The role of electric interaction in the retention index concept
      H. LAMPARCZYK

      Universal interaction indices for GLC, HPLC and TLC. Chromatographia 20, 283-288 (1965). Proposal of universal interaction indices used in TLC, GLC and HPLC, which can be easily calculated from commonly used retention parameters, such as Rf values, relative retention times etc. Presentation of the calculation examples for polycyclic aromatic hydrocarbons and n-alkanes. Demonstration of the application of the electric interaction indices for studying the retention mechanism.

      Keywords:
      Classification: 2b, 5a, 5b
      63 007
      Study of dispersive and inductive electrostatic interactions in reversed-phase thin-layer chromatography
      H. LAMPARCZYK*, R.J. OCHOCK, (*Med. Acad., Fac. Pharm., K. Marksa 107, PL-80416 Gdansk, Poland)

      Chromatographia 25, 643-646 (1988). Investigation of the retention behavior of a group of polycyclic aromatic hydrocarbons having nearly equal ionization potentials but different molecular polarizability values, using reversed-phase TLC on octadecyl-bonded silica with normal alcohols. Discussion of the relationship between retention parameters and molecular polarizabilities of the solutes and solvents.

      Keywords:
      Classification: 2c, 5b
      70 047
      The possible role of charge transfer in the chromatographic behavior of model carcinogens
      P.M. QURESHI*, M. VARSHNEY, (*Dept. of Chemistry, Aligarh Muslim Univ., Aligarh 202 002, India)

      J. Planar Chromatogr. 4, 497-498 (1991). TLC of 12 PAH’s (anthracene, phenanthracene, pyrene, benz(a)anthracene, benzo(a)pyrene, perylene, chrysene, dibenz(a,h)anthracene, benzo(e)pyrene, triphenylene, coronene) on cellulose acetate with ethanol - toluene - water 17:4:4.

      Classification: 5b
      79 028
      Retention behavior of model solutes on mixed silica-magnesia adsorbents by TLC
      M. WAKSMUNDZKA-HAJNOS*, B. WRONSKA, (Dept. Inorg. & Anal. Chem., Med. Acad., 20-081 Lublin, Poland)

      Comparison with the adsorption properties of Florisil. Chromatographia 43, 405-412 (1996). Examination of the retention behavior of positional isomers and polyaromatic hydrocarbons on mixed silica - magnesia layers with various binary eluents. Investigation of the influence of magnesia content in mixed adsorbents and concentration of polar modifier in the eluent on retention. Comparison of the results with those obtained on Florisil using the same eluent by RM correlation. Comparison of chemical character and distribution of active centers on Florisil and mixed adsorbent surfaces by analysis of the structural effects of solutes and the position of points on RM correlation diagrams.

      Keywords:
      Classification: 2c, 5b