Cumulative CAMAG Bibliography Service CCBS

Our CCBS database includes more than 11,000 abstracts of publications. Perform your own detailed search of TLC/HPTLC literature and find relevant information.

The Cumulative CAMAG Bibliography Service CCBS contains all abstracts of CBS issues beginning with CBS 51. The database is updated after the publication of every other CBS edition. Currently the Cumulative CAMAG Bibliography Service includes more than 11'000 abstracts of publications between 1983 and today. With the online version you can perform your own detailed TLC/HPTLC literature search:

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      129 063
      Automated piezoelectric spraying of biological and enzymatic assays for effect-directed analysis of planar chromatograms
      E. AZADNIYA, Gertrud E. MORLOCK* (*Institute of Nutritional Science, Justus Liebig University Giessen, and TransMIT Center of Effect-Directed Analysis, Giessen, Germany;

      J Chromatogr A, 1602, 458–466 (2019). HPTLC of caffeine, physostigmine (alkaloids) and hydroethanolic extract of Peganum harmala seeds (Nitrariaceae, Zygophyllaceae) on silica gel prewashed twice with methanol – water 3:1, followed by 1 h drying at 120 °C. Separation, after 5 min chamber saturation, with ethyl acetate – methanol – ammonia (25%) 85:11:4 (basic mobile phase) or ethyl acetate – toluene – formic acid – water 16:4:3:2 (acidic mobile phase, requiring neutralization with phosphate-citrate buffer). Derivatization with Dragendorff’s reagent and with anisaldehyde sulfuric acid. Effect-directed analysis by spraying A) with Gram-negative bioluminescent Aliivibrio fischeri suspension for antibacterial activity (caffeine was used as standard); B) with acetyl- and butyryl-cholinesterase (AChE / BChE) solutions for enzymatic inhibition. For AChE and BChE asssays, classical immersion into the enzyme solutions was also used for comparison, and inhibition densitometry for active analytes was performed by inverse scan measurement (fluorescence without optical filter) at 546 nm using a mercury lamp; activity was expressed as physostigmine equivalents. Active bands were eluted (only after basic MP) with methanol through the oval elution head of a TLC-MS interface pump, into a quadrupole-Orbitrap mass spectrometer. Full scan mass spectra (m/z 50−750) in positive ionization mode were recorded using heated electrospray ionization (HESI, spray voltage 3.5kV, capillary temperature 270°C). By comparison to literature, AChE inhibitors (also active against A. fischeri) were assigned to be harmine, harmaline and ruine (β-carboline alkaloids), and BChE inhibitors were harmol (same class) and vasicine and deoxyvasicine (quinazoline alkaloids, also called peganine and deoxypeganine). Piezoelectric spraying had the following advantages over automated immersion: (1) it covered the whole plate surface; (2) required much lower volumes of solutions; (3) applied always fresh enzyme or reagent solutions, thus avoiding gradual inactivation; (4) avoided zone distortions, shifts or tailings occurring during immersion or withdrawal of the plate, or due to the hydrophilicity of compounds.

      Classification: 3e, 4e, 22, 32e
      123 052
      Visualization reagent for the identification of phthalates
      A. BAJAJ*, C. JOHN, A. CHAUHAN, R. TRIPATHI (*Central Forensic Science Laboratory, Plot No 2, Sector 36 A, Dakshin Marg, Chandigarh 160036, India,

      J. Planar Chromatogr. 32, 55-57 (2019). TLC of dimethyl phthalate, diethyl phthalate, di-n-butyl phthalate, diisobutyl phthalate, diallyl phthalate, and bis(2-ethylhexyl) phthalate on silica gel with petroleum ether - ethyl acetate - phosphoric acid 9:1:1. Detection by spraying with 0.1 % 2,6-dichlorophenolindophenol with the instantaneous appearance of pink-colored zones.

      Classification: 3e, 9
      100 012
      Coralyne cation, a fluorescent probe for general detection in planar chromatography
      Elena MATEOS, V.L. CEBOLLA*, L. MEMBRADO, J. VELA, Eva GALVEZ, Muriel MATT, F.P. COSSIO (*Instituto de Carboquímica, CSIC, P.O. Box 549, 50080 Zaragoza, Spain;

      J. Chromatogr. A 1046 (2), 251-257 (2007). Fluorescence scanning densitometry of various analytes on HPTLC silica gel plates impregnated with a solution of coralyne cation, based on the increase or decrease, that the corresponding analyte induces on native coralyne emission at a given excitation wavelength. Compared to a procedure previously described for berberine cation, and Reichardt's dye probes, the sensitivity of coralyne in HPTLC detection of non-fluorescent, structurally different analytes (e.g. long-chain alkanes, alcohols, alkylbromides, neutral lipids) is superior.

      Classification: 3e
      112 012
      Modified ninhydrin reagents to detect colors of amino acid zones on thin-layer chromatographic plates
      A. SINHABABU*, S. BASU (*Natural Product Laboratory, Department of Chemistry, The University of Burdwan, Burdwan – 713 104, West Bengal, India,

      J. Planar Chromatogr. 26, 517-520 (2013). TLC of amino acids on silica gel with 1-propanol - water 7:3. Detection by spraying with (1) 1 % 2-hydroxybenzenecarbaldehyde solution in propanone; or (2) 1 % 4-bromobenzenecarbaldehyde solution in propanone (2), followed by heating at 110 ºC for 10 min. After cooling, all plates (treated with (1) or (2)) were sprayed with ninhydrin (indane-1,2,3-trione monohydrate) reagent and air dried. Amino acid zones in various shades from yellow to brownish-red were detected (detailed table provided in paper). Plates were heated again at 110°C for 10 min and color changes were recorded. The LOD were determined visually by comparison of standard solutions (1 mg/L) and further dilution until no zone was seen. LOD for (1) and (2) were in the range of 0.1-1.0 µg/zone and 0.01-1.0 µg/zone, respectively.

      Classification: 3e, 18a
      58 072
      Techniques for quantitative high-performance thin-layer chromatographic analysis for nonfluorescent compounds as exemplified by bile acid determination
      R. KLAUS

      J. Chromatogr. 333, 276-287 (1985). HPTLC of lacking native fluorescence but which can be converted to fluorescent derivatives: cholic acid, deoxycholic acid, hydroxycholic acid, glycocholic acid, lithocholic acid and taurocholic acid on silica with isooctane - ethyl acetate - formic acid - diisopropyl ether 5:4:1:1. Derivatization with 5 % perchloric acid in methanol.

      Classification: 3e, 13d
      65 033
      Straightforward gas phase derivatization in a novel aluminum-aluminate reactor
      R.I. RIEDER, R.E. KAISER*, (*Inst. for Chromatography, P.O. Box 1141, D-6702 Bad Dürkheim)

      J. Planar Chromatogr. 2, 62-64 (1989). Post-chromatographic derivatization, viz. gas phase derivatization, mainly of polar structures, in an aluminum-aluminate reactor to give fluorescent products. Examples of cola, wine, orange juice etc. were derivatized to fluorescent spots, which were measured by in situ scanning.

      Classification: 3e
      68 119
      Localization of amino acids on thin-layer chromatograms with acetyl acetone - formaldehyde reagent
      M.B. DEVANI*, C.J. SHISHAO, S.A. SHAH, K.P. SONI, R.S. SHAH, (*Dep. Pharm. Chem., L.M. Coll. Pharm., Ahmedabad - 380009, India)

      J. Chromatogr. 537, 494-496 (1991). TLC of 20 amino cards on silica with butanol - acetic acid - water 40:5:7. Detection by spraying with a freshly prepared solution of formaldehyde (37%, 15 mL) + acetyl acetone (7.8 mL), diluted to 100 mL with acetate buffer (pH 4.7), and by heating at 100 °C for 10 min. Detection limits, 0.26 x 10-4 - 6.9 x 10-4 mmol. Comparison of the sensitivity of this reagent with ninhydrin reagent.

      Classification: 3e, 18a
      77 027
      Pyridine-2-aldehyde-2-furoylhydrazone - a new visualization reagent for cation separation by TLC
      C. MARUTOIU*, V. COMAN, N. LUTA, R. SEMENIUC, (Inst. of Chem. "Raluca Ripan", 30 Fantanele Street, RO-3400 Cluj-Napoca, Romania)

      J. Planar Chromatogr. 9, 212-214 (1996). TLC of 12 toxic cations (Fe, Co, Ni, Cd, Cu, Pb, Hg, Hg2, Cr, Zn, Sb, Mn) on a mixture of DEAE cellulose and silica with isopropanol - ethanol - 5 N HCl 5:5:1. Identification with 0.1 % ethanolic solutions of pyridine-2-aldehyde-2-furoylhydrazone and rubeanic acid.

      Classification: 3e, 33a