Part 2. Chromatographic applications. Anal. Chim. Acta 227, 155-163 (1989). TLC of coumarin-6-sulphonyl chloride derivatized amino acids on silica with chloroform – ethyl acetate – methanol – acetic acid 20:20:9:1. Detection by absorbance at 320 nm, or by spraying with ethanol, by fluorescence 366/>500 nm at 77°K. Detection limits in the ng level of analyte for both procedures.

Anal. Biochem. 217, 335-337 (1994). TLC of cyclic peptides on silica with butanol - acetic acid - water 4:1:1. Immersion of the plates in 20% trichloroacetic acid solution for 10 min, and in 0.3% aqueous Serwa Blue W solution for 5 min with gentle shaking. Detection sensitivity, less than 1 µg (0.7 nmol) peptide per spot.

J. Planar Chromatogr. 10, 63-67 (1997). TLC of unsaturated fatty acids (cis-6-, cis-9-, cis-11-, cis-12-, cis-13-, trans-9-, trans-11-octadienoic acid, cis-6,9,12- and cis-9,12,15-octadecatrienoic acid) on silica, silica-kieselguhr and silica impregnated with a 10% solution of paraffin in hexane. Eluents were heptane - acetone 4:1 for adsorption TLC and acetic acid - water 9:1 for partition TLC. Visualization with aqueous solutions (0.5 g/L) of aniline blue, alkaline blue, brilliant green, and neutral red; and in 2% aqueous sodium hydroxide solutions (0.5 g/L) of bromothymol blue, thymol blue, bromophenol blue, phenol red, helasol green, bromocresol green, and brilliant cresyl blue. The most advantageous visualizing effects in adsorption and partition TLC were obtained with aniline blue. Detection limits 2-3 µg of unsaturated fatty acids.

J. Planar Chromatogr. 14, 462-463 (2001). TLC of 22 amino acids on silica gel with n-propanol - water 7:3. Detection by spraying with a modified ninhydrin reagent resulting in a variety of distinguishable colors with most amino acids, and with high sensitivity: Visualization by spraying with 1% 4-hydroxybenzaldehyde in acetone (reagent 1) and after drying, heating at 110°C for 10 min, cooling, spraying with 0.25% ninhydrin in acetone (reagent 2).

J. of Chromatogr. A 1218 (19), 2684-2691 (2011). Review on TLC/bioautography. Discussion of three versions of bioautography, i.e. contact, immersion and direct bioautography. The focus is put on direct bioautography and many applications are quoted, not only for testing various groups of compounds, but also for investigating biochemical processes and factors influencing bacterial growth. Various related methods can be included into direct bioautography, of which TLC-bioluminescence screening is the most promising one.

R.E.Kaiser (Ed) "Proc. of the 3rd mt. Symp. on Instr. HPTLC, Wuerzburg", Bad Duerkheim 1985, 281-311. Strategy of pre-and postchromatographic derivatization reactions with different chromophores or fluorophores. Examples of derivatization procedures for quantitative determination: diethylstilbestrol, ethinylestradiol, gentamycins, antiepileptics, selenium in human serum. Description of the optimization of sample preparation steps. Statistical model for the evaluation of systematic errors.

Chinese J. Chem. 4, 35-36 (1987) (Huaxue Tongbao). Use of N,N-dimethyl-a-naphthylamine-5-sulfonyl chloride as a pre-chromatography derivatizing agent for some pesticides and herbicides. Spraying with tris(2-hydroxyethyl)-amine or paraffin oil after development. Detection by fluorodensitometry at 320 nm excitation and 500 nm emission.

An overview. Proc. 6th Int. Symp. Instrum. Planar Chromatogr., (Interlaken 1991), Inst. Chromatogr., Bad Dürkheim, FRG, 71-79 (1991). Overview of a new postchromatographic derivatization method; special devices for contact reagent transfer were described.