Cumulative CAMAG Bibliography Service CCBS

Our CCBS database includes more than 11,000 abstracts of publications. Perform your own detailed search of TLC/HPTLC literature and find relevant information.

The Cumulative CAMAG Bibliography Service CCBS contains all abstracts of CBS issues beginning with CBS 51. The database is updated after the publication of every other CBS edition. Currently the Cumulative CAMAG Bibliography Service includes more than 11'000 abstracts of publications between 1983 and today. With the online version you can perform your own detailed TLC/HPTLC literature search:

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      130 043
      Development of the first universal mixture for use in system suitability tests for High-Performance Thin Layer Chromatography
      T.K. Tiên DO*, M. SCHMID, M. PHANSE, A. CHAREGAONKAR, H. SPRECHER, M. OBKIRCHER, E. REICH (*CAMAG, Muttenz, Switzerland; tien.do@camag.com)

      J Chromatogr A 1638, 461830 (2021). The purpose was to find the first universal HPTLC mixture (UHM), a mixture of reference compounds that could be used for the system suitability test (SST) for the full RF range in all HPTLC experiments.
      (Part 1) UHM composition: First, 56 organic molecules, detectable without derivatization, were tested on HPTLC silica gel with 20 different mobile phases (MP) belonging to different Snyder’s selectivity groups and with several polarity indices. Visualization under UV 254 nm and 366 nm. Densitometry scanning at 254 nm in absorption mode, and at 366 nm in a fluorescence mode (mercury lamp 366 nm, with wavelength filter <400 nm). For selected bands, spectra were recorded in absorbance-reflectance mode (wavelength range 190 – 450 nm, deuterium and tungsten lamp). This procedure allowed 8 molecules to be selected for their better spot resolution and for their specific RF values (at least 3 different values distributed throughout the full RF range for each MP). The final composition of UHM was: thioxanthen-9-one (0.001 %), guanosine (0.05 %), phthalimide (0.2 %), 9-hydroxyfluorene, octrizole, paracetamol, sulisobenzone and thymidine (each 0.1 %), in methanol.
      (Part 2) UHM validation: Afterwards, UHM was submitted again to a panel of HPTLC assays with always two MP: (A) toluene – methanol – diethylamine 8:1:1; (B) ethyl acetate – formic acid – water 15:1:1; and for each MP, the means, standard deviation and 95 % confidence intervals of the RF values were calculated. (a) UHM was validated for intermediate intra-laboratory precision, as well as for inter-laboratory reproducibility, with ΔRF 0.045. (b) The capacity of UHM to detect small variations was demonstrated by significant changes in at least some RF values, when separation was deliberately performed at different levels of relative humidity (0 %, 33 %, 75 %, 100 %), or with smaller humidity variations (7 % compared to 0–5 %, and 49 % compared to 33 %), or when performing vs. omitting the 10min chamber pre-saturation, or when modifying the MP (+/-10% of one solvent at each time). These response characteristics (the opposite of robustness) made UHM a powerful tool for SST. (c) Finally, UHM stability was studied with UHM aliquots under several storage conditions (-78 °C, -20 °C, 4 °C, room temperature, 45 °C; or 40 °C with 75 % relative humidity) and durations (2 weeks or 2 months). The densitometric peak profiles at 254 nm were compared to those of the fresh compounds, qualitatively (RF value, UV spectrum) and quantitatively (peak area). UHM was stable at room temperature or below, for 2 months (at higher temperature, guanosine, phthalimide and paracetamol degraded).

      Classification: 2f, 3g, 7, 21a, 23e, 24
      123 013
      High performance thin layer chromatography hyphenated with electrospray mass spectrometry for evaluation of nucleobases in two traditional Chinese medicinal mushrooms: A metabolomic approach
      J. MISHRA, A. BHARDWAJ, M. PAL, R. RAJPUT, K. MISRA (*Defence Institute of Physiology and Allied Sciences, Delhi 110054, India, kmisra99@yahoo.com)

      J. Liq. Chromatogr. Relat. Technol. 41, 15-16 (2019). HPTLC of thymine (1), uracil (2), adenine (3), cytosine (4), guanine (5) and guanosine (6) in Ganoderma lucidum and Cordyceps sinensis on silica gel with dichloromethane - methanol - formic acid 160:45:16. Quantitative determination by absorbance measurement at 254 nm. Identification of nucleobases in the samples was reconfirmed by hyphenated HPTLC-MS. The hRF values for (1) to (6) were 83, 73, 46, 32, 23 and 10, respectively. The intermediate precision was below 5 % (n=3). 

      Classification: 21a
      121 065
      Determination of caffeine, theobromine and theophylline in Mate beer and Mate soft drinks by high-performance thin-layer chromatography
      Claudia OELLIG*, J. SCHUNCK, W. SCHWACK* (*Inst. of Food Chem., Univ. of Hohenheim, Garbenstrasse 28, 70599, Stuttgart, Germany)

      J. Chromatogr. A 1533, 208-212 (2018). Development of a rapid method for the determination of caffeine, theobromine and theophylline in Mate beer and Mate soft drinks. Sample preparation through filtration followed by degassing, dilution with acetonitrile in the case of Mate beers for protein precipitation and centrifugation. HPTLC of the extracts on silica gel with acetone – toluene – chloroform 4:3:3, detection and quantification by densitometry at UV 274 nm. The LOD and LOQ were 1 and 4 ng/zone for caffeine, theobromine and theophylline, respectively, and recoveries were close to 100%. Demonstration of the method by applying to the analysis of the concentrations of three methylxanthines in Mate beers as well as Mate beers and Mate soft drinks available from the market proved to be sensitive, accurate and reliable.

      Classification: 21a
      64 176
      Synthesis and biological evaluation of certain 3-ß-D-ribofuranosyl-1,2,4-triazolo(4,3-b)pyridazines related to formycin precursors
      Y. KANG*, S.B. LARSON, R.B. ROBLINS, G.K. REVANKAR, (*ICN Nucleic Acid Research Inst., 3300 Hyland Av., Costa Mesa, California 92626, USA)

      J. of Medicinal Chemistry 32, 1547-1551 (1989). TLC of nucleosides on silica with ethyl acetate - ethanol 6:1 and 9:1. Detection by UV and with 10% sulfuric acid in methanol followed by heating.

      Classification: 21a
      75 078
      New cardiac glycosides containing 2-deoxyhexoses from the defensive of adult Chrysolina banksi (Coleoptera
      D. DALOZE *, F. BROEDERS, J.C. BRAEKMAN, J. ARANJO, J.M. PASTEELS, (*Lab. of Bio-organic Chem., CP 160/07 Fac of Sci., Univ. of Brussels, Belgium)

      Biochemical Systematics and Ecology 23, 113-119 (1995). TLC of cardenolides on silica with dichloromethane - methanol 8:2. Visualization under UV 254 nm for adenosine of with Kedde reagent for cardenolides

      Classification: 14, 21a
      85 020
      A rapid, sensitive, and nonradioactive method for assay of FHIT Ap3A hydrolase activity by fluorescence thin-layer chromatographic image analysis
      LIN JI*, BINGLIANG FANG, J.A. ROTH, (Univ. Texas, MD Anderson Canc. Ctr., Dept. Thorac. & Cardiovasc. Surg., Sedt. Thorac. Mol. Oncol., 1515 Holcombe Blvd., Houston, TX 77030, USA; lji@notes.mdacc.tmc.edu)

      Anal. Biochem. 271, 114-116 (1999). TLC of 10 to 20 (l of the assay mixture containing the SYBR Green II-bound reaction products AMP and ADP on silica with dioxane - NH3 - water 6:1:4. The resulting fluorescence image was directly scanned and quantitated.

      Keywords:
      Classification: 3e, 21a, 32f
      56 096
      Separation of adenosine and its derivatives by thin-layer chromatography on silica gel
      M. FIGUEIRA, J. RIBEIRO

      J. Chromatogr. 325, 317-322 (1985). TLC of tritiated adenosine and its derivatives on silica with butanol- acetone - acetic acid -5 % NH3 - water 9:3:2:2:4, butanol - acetone - acetic acid - water 11:3:3:3 and numerous other similar solvent systems. Scintillation counting after scraping the sample from the plate or direct radio scanning of the plate.

      Keywords:
      Classification: 21a, 34
      64 177
      Synthesis and biological properties of purine and pyrimidine 5'-deoxy-5'-(dihydroxyphosphinyl)-ß-D-ribofuranosyl analogues of AMP, GMP, IMP and CMP
      N. RAJN*, D.F. SMEE, R.K. ROBINS, M.M. VAGHEFI, (*Nucleic Acid Research Inst., 3300 Hyland Av., Costa Mesa, California 92626, USA)

      J. of Medicinal Chemistry 32, 1307-1313 (1989). TLC of phosphonate analo- gues an silica with dichloromethane - acetone 3:2, methanol - chloroform 1:9 and isopropanol - NH3 - water 7:1:2. Detection under UV and by spraying with 10% sulfuric acid in methanol followed by heating.

      Classification: 21a