Ein neues Verfahren zum Nachweis von Substanzen in der Dünnschichtchromatographie. I. Nachweis von Flavoniden. Microwave vaporizing-technique of substances on thin-layer chromatogramms. I. Detection of flavonoids. (German). Deutsche Apotheker Zeitung 129, 2178-2179 (1989). New method for the detection of substances on thin-layer chromatogramms. The procedure consists in the vaporizing of the reagent using a microwave-oven. The advantage of the method is illustrated by the flavonoid detection TLC of flavonoids on silica with ethyl acetate - formic acid - water 8:1:1.

J. Planar Chromatogr. 3, 247-250 (1990). TLC of tryptophan on cellulose with 1-butanol - acetic acid - water 4:1:5. Detection by overpressure derivatization (OPD), which is carried out by pressing an absorbent polymeric pad (prewetted with derivatization reagent) on the TLC plate. Relative standard deviations obtained showed that OPD was more reproducible than spraying. Quantification by densitometry (absorbance at 420 and 288 nm after derivatization).

J. West China Univ. Med. Sci. (Huaxi Yaoxue Zazhi) 8, 70 - 73 (1994). Use of a new stable fluorescent reagent CGE(N), for the fluorimetric detection of p-amino-salicylic acid in tablets on silica with petrol ether - chloroform - ethyl acetate 5:3:2. Quantification by densitometry at 277 nm. Detection limit 0.38 µg.

Anal. Biochem. 271, 114-116 (1999). TLC of 10 to 20 (l of the assay mixture containing the SYBR Green II-bound reaction products AMP and ADP on silica with dioxane - NH3 - water 6:1:4. The resulting fluorescence image was directly scanned and quantitated.

J. Planar Chromatogr. 19, 252-254 (2006). TLC of 22 amino acids on silica gel with n-propanol - water 7:3. Detection by spraying with 0.25 % 2,3-dichloro-1,4-naphthoquinone in ethanol, followed by drying in the air at room temperature and heating in an oven at 110 °C for 10 min. After cooling spraying with 0.4 % isatin in ethanol. Visual detection of spot colors (varying from yellow, to orange, pink, purple, and gray). Detection limits were determined visually and ranged from 0.01 µg (cystine and arginine) to 0.30 µg (isoleucine, phenylalanine, methionine, aspartic acid, and glycine).

J. Chromatogr. A 1164 (1-2), 298-305 (2007). TLC using staining reagents is fast, versatile and sometimes the only viable method method for analyzing organic compounds without chromophores. Investigation of quantitative TLC using staining reagents in combination with modern image analysis software showed that it is possible to get reliable measurements, suitable for high-throughput screening or physical organic investigations. Illustration of the range of detection and the errors for the different parts of the process, which are largely due to the staining process but can be diminished by measuring ratios of compounds.

J. Planar Chromatogr. 26, 26-30 (2013). Four new spray reagents for the detection of amino acids were introduced: (1) 0.25 % solution of benzoic acid in ethanol, (2) 0.1 % solution of p-fluorobenzoic acid in ethanol, (3) 0.1% solution of p-chlorobenzoic acid in ethanol and (4) 0.05 % solution of p-iodobenzoic acid in ethanol. Depending on the different amino acids tested the LOD on silica gel was in the range of 0.1-1.0 µg for all four reagents. For example for cysteine the LOD was 0.1 µg with reagents (1), (3), and (4), and 0.2 µg with reagent (2).

Chromatographia 20, 683-684 (1985). TLC of some purines on silica with iso-butanol MEK - NH3 - water 8:5:4:3 and propanol - NH3 water 16:3:1. Visualization by spraying with 1 % aqueous uranyl acetate and irradiating with UV at 254 or 366 nm. Detection limit 0.01/mg.