Chinese J. Northern Pharm. 10 (12), 10-11 (2013). Yinhuang Qingre Jiaonang capsule is a TCM compound used for the treatment of respiratory tract infection, acute and chronic tonsillitis, sore throat, pneumonia, periodontitis, conjunctivitis, influenza virus infection, etc. For the quality control, HPTLC of the drug and berberine hydrochloride on silica gel (1) for Cortex Phellodendri Chinensis and Coptis chinensis Franch. with benzene – ethyl acetate – isopropanol – methanol – concentrated ammonia 12:6:3:3:1, detection by exposure to ammonia vapor; (2) for Flos Lonicerae Confusae with butyl acetate – formic acid – water 14:5:5; (3) for Fructus Aurantii (A) with ethyl acetate – formic acid – water 100:17:13 up to 3 cm, and then, (B) with toluene - ethyl acetate – formic acid – water 20:10:1:1 up to 8 cm; for all (1-3), detection at UV 366 nm. The method had been applied routinely and proved to be suited for the quality control of the medicine.

J. Planar Chromatogr. 28, 287-293 (2015). HPTLC of bacoside A in the leaves of Bacopa monnieri on silica gel with ethyl acetate - methanol - water 4:1:1. Detection by spraying with 1 % vanillin in 10 % methanolic sulfuric acid. Quantitative determination by absorbance measurement at 598 nm. The hRF value for bacoside A was 53. LOD and LOQ were 60 and 180 ng/zone, respectively. The intermediate precision was below 1 % (n=3). Recovery ranged between 97 and 100 %.

J. Planar Chromatogr. 28, 443-447 (2015). TLC of protocatechuic acid (1), chlorogenic acid (2), oleanolic acid (3) and ursolic acid (4) in the extracts of Chaenomeles speciosa on polyamide phase with dichloromethane - ethyl acetate - formic acid 5:4:1 for (1) and (2) or cyclohexane - acetone - ethyl acetate - formic acid 45:10:1 for (3) and (4). Detection by spraying with 0.04 % solution of 2,2-diphenyl-1-picrylhydrazyl in ethanol (DPPH radical reagent) to assess the antioxidant activity. The hRF values for (1) and (2) were 51 and 30, respectively.

J. Ethnopharmacol. 181, 236-251 (2016). Analysis of the botanical description, geographical location, traditional and medicinal uses, phytochemistry and pharmacological investigation, toxicological aspects, patent information and clinical studies of Symplocos racemosa. The review described methods for the determination of loturine in the bark extracts of Symplocos racemosa on silica gel with chloroform – acetonitrile – triethylamine 7:5:2, quantitative determination by absorbance measurement at 280 nm.

Chinese J. of Inform. on TCM 20 (10), 46-48 (2013). Buxin Ruanmai granule is a herbal TCM for coronary heart disease and angina pectoris. For quality control, TLC (1) for Ophiopogon japonicus, on silica gel with n-hexane – ethyl acetate – formic acid 10:10:4, detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 °C until the zones are visible, evaluation under UV 366 nm; (2) for common Macrocarpium fruit, on silica gel with ethyl acetate – ethanol – glacial acetic acid 50:10:1, detection by spraying with 5 % vanillin-sulfuric acid in ethanol 1:4 and heating at 105 °C until the zones are visible; (3) for Whitmania pigra Whitman, on silica gel with cyclohexane – ethyl acetate 4:1, detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 °C until the zones are visible, evaluation under UV 366 nm; (4) for Ginkgo biloba, on polyamide layer with 40 % acetic acid, detection by spraying with 3 % aluminium trichloride in ethanol, drying in a warm stream of air, evaluation under UV 366 nm; (5) for Polygonum cuspidatum Sieb. et Zucc., on silica gel with petroleum ether (60-90 °C) – ethyl formate – formic acid 15:5:1, detection under UV 366 nm. Quantitative determination of salidroside and loganin by HPLC.

J. of China Pharm. 23 (5), 29-31 (2014). Xiaoke Maikang Keli granule is a herbal TCM for treatment of diabetes and peripheral neuropathy etc. For quality control, TLC for (1) Achyranthes bidentata, on silica gel with chloroform – methanol 40:1, detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 ºC until the zones are clearly visualized, evaluation under white light; (2) Salvia miltiorrhiza, on silica gel with chloroform – acetone – formic acid 79:11:10, detection under UV 254 nm; (3) for Fructus Lycii, on silica gel with ethyl acetate – chloroform – formic acid 5:4:2, detection under UV 366 nm; (4) for Taxillus sutchuenensis, on silica gel with water-saturated toluene – ethyl formate – formic acid 5:3:1, detection by spraying with 5 % aluminum trichloride in ethanol, evaluation under UV 366 nm; (5) for Corydalis yanhusuo, on silica gel with petroleum ether (60-90 ºC) – ethyl acetate 1:1, detection by exposure to iodine vapors for a few seconds and evaluation under UV 366 nm; (5) for Astragalus membranaceus, on silica gel with chloroform – methanol – water 13:7:2, detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 °C until the zones are visible, evaluation under UV 366 nm.

J. Planar Chromatogr. 30, 199-204 (2017). HPTLC of sophoridine (1), matrine (2), and sophocarpine (3) in S. alopecuroides on silica gel with toluene – acetone – methanol – ammonia – water 80:30:2:5:80. Detection by spraying with bismuth potassium iodide reagent (1:1 mixture of 0.85 g of bismuth nitrate with 10 mL of glacial acetic acid and 40 mL of water, heated for dissolution, and 8 g of potassium iodide in 30 mL of water). Quantitative determination by absorbance measurement at 500 nm. The hRF values for (1) to (3) were 22, 56 and 65, respectively. Linearity ranged from 415-2491 ng/zone for (1), 424-2547 ng/zone for (2) and 410-2460 ng/zone for (3). The intermediate precision was <5 % (n=3). Recovery rate ranged from 97.8 to 98.9 % for (1), 98.3 to 100.9 % for (2) and 99.1 to 101.2 % for (3).

J. Planar Chromatogr. 30, 423-426 (2017). 2D-HPTLC of phytoestrogenic active compounds in the root of Glycyrrhiza glabra on RP-18 with hexane – ethyl acetate – acetone 9:3:2 in the first direction and acetone – water 3:2 in the second direction. Effect-direct analysis by dipping into a yeast suspension followed by incubation at 30 °C for 4 h, drying at 37 °C for 15 min and spraying with the combined reaction buffer C (20 mL reaction buffer C is mixed with 0.2 mL of a freshly prepared solution of 0.05 g/mL 4-methylumbelliferyl-ß-D-galactopyranoside in DMSO or 0.2 mL of a freshly prepared solution of 0.05 g/mL 5-bromo-4-chloro-3-indoxyl-ß-D-galactopyranoside (X-Gal) in DMSO). Fluorescence detection at UV 366 nm._x000D_