Cumulative CAMAG Bibliography Service CCBS

Our CCBS database includes more than 11,000 abstracts of publications. Perform your own detailed search of TLC/HPTLC literature and find relevant information.

The Cumulative CAMAG Bibliography Service CCBS contains all abstracts of CBS issues beginning with CBS 51. The database is updated after the publication of every other CBS edition. Currently the Cumulative CAMAG Bibliography Service includes more than 11'000 abstracts of publications between 1983 and today. With the online version you can perform your own detailed TLC/HPTLC literature search:

  • Full text search: Enter a keyword, e.g. an author's name, a substance, a technique, a reagent or a term and see all related publications
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      106 024
      Quality control standards for the roots of three plumbago species
      S. ARIYANATHAN*, A. SARASWATHY, G. RAJAMANICKAM (*Centre for Advanced Research in Indian system of Medicine (CARISM) SASTRA University, Thanjavur 613402, India, saraswathy20042000@yahoo.co.in)

      Ind. J. Pharma. Sci. 72(1), 86-91 (2010). Three species of Plumbago (Plumbaginaceae), i.e. P. zeylanica, P. carpensis, and P. rosea were studied for different physico-chemical parameters in addition to the estimation of microbial contamination, aflatoxins and pesticide residues and heavy metal content. All three species are used as herbs. The fingerprint profile of each species was compared using plumbagin as marker. Chloroform extracts of each plant were subjected to chromatography on silica gel with toluene - ethyl acetate 4:1 in a saturated twin trough chamber. Detection under UV 254 nm and 366 nm. The hRf value of plumbagin was 70. The identity of plumbagin in the samples was shown by overlay of the UV spectra. Linearity was between 200 and 1000 ng/zone. The amount of plumbagin in the three species was between 0.01 and 0.17 %.

      Classification: 6
      106 157
      Identification and separation of Cannabis sativa, Embleia ribes, Myristica fragrans and Piper longum from organic extract on silica gel surface with anionic micellar solvent system
      A. MOHAMMAD*, S. SHARMA, S. BHAWANI, R. SINGH (*Analytical Research Lab., Dept. of Applied Chem., Faculty of Eng. & Tech., Aligarh Muslim University Aligarh 202002, India)

      The Open Nutraceuticals Journal 2, 2-6 (2009) A TLC method using a micellar solution of sodium dodecyl sulfate (SDS) as mobile phase has been developed for identification of four herbals present in Jatiphaladya, a powdered herbal formulation containing Cannabis sativa, Myristica fragrans, Piper longum, and Embleia ribes. The formulation was extracted with 80 % ethanol. TLC on laboratory made plates coated with silica gel and activated at 100 °C for 60 min, with a 5 % solution of SDS as mobile phase. The resolved spots were identified by spraying with a 2 % solution of vanillin in 5 % methanolic sulfuric acid. Spots corresponding to different herbals were well resolved. Different detection reagents were evaluated, i.e. iodine, vanillin sulfuric acid, and anisaldehyde-sulfuric acid. Vanillin sulfuric acid reagent was found to be the most sensitive. Of the different surfactants used, anionic, cationic and nonionic, SDS was found to be most suitable. The most suitable pH of the mobile phase was pH 4.2-5.7, it provided optimum resolution of zones.

      Classification: 32e
      107 120
      Development and validation of a stability-indicating HPTLC method for analysis of 3-acetyl-11-keto-beta-boswellic acid in a herbal extract and a nanoparticles formulation
      A. GOEL*, R. GOEL, G.K. JAIN, R.M. SINGH, F.J. AHMAD, G.N. SINGH (*Government of India, Central Indian Pharmacopoeia Laboratory, Ministry of Health and Family Welfare, Ghaziabad Uttar Pradesh, India)

      Acta Chromatographica 20(3), 497-511 (2008). HPTLC of 3-acetyl-11-keto-beta-boswellic acid (AKBA) on silica gel with toluene – ethyl acetate 7:3 at room temperature (25 ± 2 °C) in a twin-trough chamber with chamber saturation. Quantification of AKBA (hRf 52) by densitometry in absorbance mode at 250 nm. The linearity was in the range of 200–1200 ng/band (r=0.9989), recovery was 99.4-100.2 %, and the limits of detection and quantification were 3 and 9 ng/band, respectively. AKBA was subjected to various stress conditions: acid and alkali hydrolysis, oxidation, photodegradation, and dry and wet heat treatment. The degradation products were separated from the pure drug with significantly different hRf values.

      Classification: 32c
      107 151
      Simultaneous quantification of diterpenoids in Premna integrifolia using a validated HPTLC method
      D. YADAV, N. TIWARI, M. GUPTA* (*Analytical Chemistry Department, Central Institute of Medicinal and Aromatic Plants, Uttar Pradesh 226015, India, guptammg@rediffmail.com)

      J. Sep. Sci. 34, 286-291 (2011). HPTLC of 1beta,3alpha,8beta-trihydroxy-pimara-15-ene (1), 6alpha,11,12,16-tertahydroxy-7-oxo-abieta-8,11,13-triene (2) and 2alpha,19-dihydroxy-primara-7,15-diene (3) in the root bark of Premna integrifolia on silica gel with hexane – acetone – ethyl acetate 3:1:1. Detection by dipping into vanillin-sulfuric acid reagent (2 g vanillin in 190 mL ethanol with 10 mL sulfuric acid) followed by air drying and heating for 3 min at 110 °C. Quantitative determination by absorbance measurement at 475 nm. The hRf values of (1), (2) and (3) were 58, 44, and 32, respectively and selectivity regarding matrix was given. Linearity was between 1-10 µg/spot for (1), (2) and (3), respectively. The limits of detection were found to be 230, 106 and 336 ng/band for compounds (1), (2) and (3), respectively, whereas the limits of quantification were 769, 354 and 1122 ng/band, respectively. Inter- and intraday precisions were 0.9-1.3 % and 1.1-1.2 %, respectively. The average recoveries for compounds (1) to (3) were found to be 100.6, 103.9 and 97.6 %, respectively, within the acceptable %RSD.

      Classification: 32e
      108 085
      (Study on quality standard for Wuyangbuxin capsules) (Chinese)
      J. FENG (Feng Jinghui)*, D. SUN (Sun Dayong), J. LI (Li Junping), M. JIANG (Jiang Mingzhang) (*Yantai Dayang Pharm. Co. Ltd., Yantai 265500, China)

      J. of Qilu Med. & Pharm., 30 (2), 91-93 (2011). TLC of the extracts of Wuyangbuxin capsules 1) for Polygonium multiflorum, on silica gel with the upper phase of toluene - ethyl acetate - formic acid 10:1:1, detection under UV 365 nm; 2) for Licorice, on silica gel with petroleum ether (60-90 °C) - toluene - ethyl acetate - glacial acetic acid 20:40:14:1, detection by spraying with 10 % sulfuric acid in ethanol and heating until zones were detected; 3) for Milkvetch root, on silica gel with the lower phase of chloroform - methanol - water 13:6:2 at 10 °C, detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 °C until zones were detected; 4) for Epimedium herb on silica gel with ethyl acetate - butanone - formic acid - water 10:1:1:1, detection by spraying with AlCl3 reagent, heating at 105 °C until zones were detected and viewing under UV 365 nm. Identification by fingerprint comparison with the standards of the individual drug components.

      Classification: 32e
      108 116
      Chemical fingerprinting of Turnera diffusa and closely related genera by high-performance thin-layer chromatography
      A.S. RAO*, J. SHAO, T.J. SMILLIE, I.A. KHAN (*National Center for Natural Products Research, Research Institute of Pharmaceutical Sciences, The University of Mississippi, University, MS 38677, USA)

      Planta Med. 74, 352-353 (2008). HPTLC of tetraphyllin, turneradiffusin, beta-arbutin, terniflorin, echinaticin, turneradin and methanolic extracts of Turnera diffusa on silica gel with ethyl acetate - acetic acid - water 190:10:1. Quantitative determination by densitometric absorbance measurement at 254 nm.

      Classification: 32e
      108 143
      Sesquiterpenoids from Inula britannica
      J. YANG (Yang Jun-Li), R. WANG (Wang Rui), L. LIU (Liu Lei-Lei), Y. SHI* (Shi Yan-Ping) (*State Key Laboratory of Applied Organic Chemistry, Lanzhou University, Lanzhou, people’s Republic of China;Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, People’s Republic of China. shiyp@licp.cas.cn)

      Planta Med. 77, 362-367 (2011). Analytical and preparative TLC of 19 sesquiterpenoids (e.g., ivalin, 6ß-hydroxytomentosin, 4-epipulchellin 2-O-acetate, and bigelovin) on silica gel with chloroform - ethyl acetate 8:1 and chloroform - methanol 15:1. Detection under UV 254 nm and by spraying with 98 % sulfuric acid - ethanol 1:19, followed by heating.

      Classification: 32e
      109 037
      An improved method for thin-layer chromatographic analysis of saponins
      O. SHARMA*, N. KUMAR, B. SINGH, T. BHAT (*Biochemistry Laboratory, Indian Veterinary Research Institute, Regional Station, Palampur 176 061, Himachal Pradesh, India, omsharma53@yahoo.com)

      Food Chemistry 132, 671-674 (2012). TLC of saponins on silica gel with n-butanol - water - acetic acid 12:2:1. Detection by dipping into a suspension of sheep erythrocytes for 20 s, then plates were taken out and held vertically for 30 s. White spots against a pink background appeared. The plate was immersed in phosphate-buffered saline for 30 s to remove excess blood on the plate surface and again held vertically for 30 min. The method is simple, specific, convenient and time saving for analysis of saponins by TLC for purification, chemoprofiling of plants, and nutraceutical applications.

      Classification: 14