Cumulative CAMAG Bibliography Service CCBS

Our CCBS database includes more than 11,000 abstracts of publications. Perform your own detailed search of TLC/HPTLC literature and find relevant information.

The Cumulative CAMAG Bibliography Service CCBS contains all abstracts of CBS issues beginning with CBS 51. The database is updated after the publication of every other CBS edition. Currently the Cumulative CAMAG Bibliography Service includes more than 11'000 abstracts of publications between 1983 and today. With the online version you can perform your own detailed TLC/HPTLC literature search:

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      111 084
      (Determination of partition coefficient of alkaloids of Coptis chinensis in the solvent system of high speed countercurrent chromatography by thin-layer chromatography and fluorescence spectrophotometry) (Chinese)
      J. JI (Ji Jia) (Pharm. Prepar. Dep., Zhengzhou Municip. People’s Hosp., Henan, Zhengzhou 450000, China)

      Chinese J. of Med. Guide 2 (9), 109-110 (2012). Coptis chinensis is a herbal TCM drug for relieving internal heat or fever. High speed countercurrent chromatography (HSCCC) is frequently applied to analyse the alkaloids of Coptis chinensis. To optimize the solvent system of HSCCC the method for determination of the partition coefficient of Coptis chinensis alkaloids was done by TLC and fluorescence spectrophotometry. The major alkaloids are coptisine, berberine, palmatine and epiberberine. The crude drug was extracted with ethanol and contained 9.2 % coptisine, 18.5 % berberine, 4.0 % palmatine and 3.5 % epiberberine. TLC on silica gel with cyclohexane – ethyl acetate – isopropanol – methanol – water – triethylamine 6:7:2:3:1 with chamber saturation for 20 min with ammonia vapors, detection at UV 366 nm. The zones were quantitatively scraped off the layer and eluted with ethanol for fluorescence spectrophotometry at UV 366 nm. Quantification by external standard calibration over the linearity range of 0.5-2.5 g/L for coptisine, berberine and epiberberine with standard addition recovery of 98.3 % (%RSD = 1.9 %, n=6), 95.3 % (%RSD = 3.4 %, n=6), 103.9 % (%RSD = 2.6 %, n=6), respectively; and 1.0-3.0 g/L for palmatine with standard addition recovery of 97.6 % (%RSD = 3.7 %, n=6). Calculation of the partition coefficient, i.e. the ratio of observed values of the analytes from the two phases, respectively, with the results for coptisine 2.20, berberine 0.29, palmatine 0.21 and epiberberine 0.61.

      Classification: 32e
      111 105
      (Separation and identification of chlorogenic acid in Cleavers by thin-layer chromatography) (Chinese)
      P. LIU (Liu Pinjian)*, J. CHEN (Chen Jian), Y. REN (Ren Yanan) (*Jiujiang Inst., School of Med., Jiangxi, Jiujiang 332000, China)

      J. of Guangzhou Chem. Industry. 40 (24), 122-123 (2012). Cleavers (Galium aparine) is a herb used as a key component drug in various TCM formulations for the treatment of tumours, dysentery, mastitis, urinary tract infections, gallbladder diseases etc. For quality control and to guarantee the medication safety of relevant formulations, TLC of chlorogenic acid, the main active ingredient, on silica gel with ethyl acetate – formic acid – water 14:1:1, detection 1) by viewing under UV 366 nm; 2) by spraying with 2 % ferric chloride in ethanol and heating mildly then viewing in daylight; 3) by densitometry at 585 nm.

      Classification: 32e
      111 137
      (Study on the method for the quality control of Danbaijing capsules by thin-layer chromatography) (Chinese)
      L. WU (Wu Lizhong)*, L. WANG (Wang Luhong), H. NIU (Niu He) (*Jilin Oilfield General Hosp., Jilin 138000, China)

      Chinese J. Mod. Drug Appl. 6 (9), 124-125 (2012). Danbaijing capsules are a herbal TCM for treating intractable proteinuria. For quality control, TLC on silica gel 1) for Radix Astragali and the standard astragaloside A, with chloroform – methanol – water 13:7:2, detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 °C, viewing in daylight; 2) for Centella asiatica and the standard asiaticoside, with chloroform – methanol – water 14:6:1, detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 °C, viewing in daylight.

      Classification: 32e
      111 153
      (Study on the method for the quality control of Huoxiang Qushu Soft capsules by thin-layer chromatography) (Chinese)
      G. ZHENG (Zheng Guofeng) (Fuyang Inst. for Food & Drug Contr., Anhui, Fuyang 236015, China)

      J. Strait Pharm. 24 (9), 53-55 (2012). Huoxiang Qushu Soft capsules are an approved herbal TCM preparation used for promoting perspiration, reducing internal heat, regulating spleen and stomach. For quality control, TLC on silica gel 1) for Herba Pogostemonis, with petroleum ether (30-60 ºC) – ethyl acetate – glacial acetic acid 85:15:1, detection by spraying with 5 % ferric chloride in ethanol and heating at 105 °C, viewing in daylight; 2) for Radix Angelicae Dahuricae, with petroleum ether (30-60 ºC) – diethyl ether 3:2, detection under UV 366 nm; 3) for Folium Perillae, with ethyl acetate – methanol – formic acid – water 18:1:2:1, detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 °C, viewing under UV 366 nm; 4) for Rhizoma Atractylodis, with petroleum ether (30-60 ºC) – acetone 9:2, detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 °C, viewing in daylight; 5) for Rhizoma Pinelliae Preparatum, with petroleum ether (30-60 ºC) – ethyl acetate – acetone – formic acid 60:12:10:1, detection under UV 254 nm; 6) for Poria, with toluene – ethyl acetate – formic acid 40:10:1, detection by spraying with 2 % vanillin in sulfuric acid – ethanol 1:200 and heating at 105 °C, viewing in daylight; 7) for Radix Glycyrrhizae, with ethyl acetate – formic acid – glacial acetic acid – water 15:1:1:2, detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 °C, viewing under UV 366 nm.

      Classification: 32e
      112 075
      (Study of the method for identification of Flos Lonicerae and Astragalus mongholicus in Shaoshang Heji mixture by thin-layer chromatography) (Chinese)
      SH. CHENG (Cheng Shouxian) (Manufacturing Lab. of Liling Municip. Trad. Chinese Med. Hosp., Hunan, Liling 412200, China)

      Chinese J. Mod. Drug Appl. 6 (11), 124-125 (2012). Shaoshang Heji mixture is a herbal TCM preparation for treating burns with significant curative effects. For quality control, TLC on silica gel 1) for Flos Lonicerae and the standard chlorogenic acid, with ethyl acetate – formic acid – water 16:3:6, detection under UV 366 nm; 2) for Astragalus mongholicus and the standard astragaloside A, with the lower phase of chloroform – methanol - water 13:7:2, detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 ° for 5 min, evaluation in daylight and under UV 366 nm.

      Classification: 32e
      112 094
      (Evaluation of the uncertainty of thin-layer chromatographic determination of oleanolic acid in Kangbingdu Jiaonang capsules) (Chinese)
      Y. LU (Lu Yiling) (Hangzhou Municip. Inst. for Drug Contr., Zhejiang, Hangzhou 310017, China)

      Chinese J. of Herald of Med. 32 (2), 242-244 (2013). TLC is widely used for the analysis of active components in TCM preparations. The degree of accuracy of the detection results can be confirmed by uncertainty evaluation, by which the critical value of measurement results are judged. The uncertainty assessment for the measurement process and experimental data is part of the information which has to be submitted with drug inspection reports. In this paper the TLC determination of the oleanolic acid content of Kangbingdu Jiaonang capsules is used as an example to study the uncertainty in order to improve the accuracy and reliability of data analysis and the measuring results. TLC of the extracts and the standard oleanolic acid, on silica gel with chloroform – methanol 20:1, detection by spraying with 10 % sulfuric acid in ethanol and heating at 100 °C until the zones are visible in daylight. Quantification by densitometry at 520 nm. In summary the main factors affecting the uncertainty of the results are 1) the data calculated from the calculation curve, the uncertainty due to the least squares fitting curve, the purity of the standard sample used, weighing operation and the glassware taken; 2) the uncertainty of the sample application volume due to the application tools employed; 3) the uncertainty of the sample dilution factor due to the operation proficiency. A method for the calculation of the uncertainty corresponding to above factors in TLC determination of oleanolic acid in Kangbingdu Jiaonang capsules is presented, so that the accuracy and reliability of the results can be evaluated scientifically and an objective basis is provided to improve the executive procedure employed.

      Classification: 32e
      112 119
      (Study of the method for the identification of Chinese Eupatorium L
      SH. XIE (Xie Shiwei) (Dongguan Municip. Inst. for Drug Contr., Guangdong, Dongguan 523109, China)

      root by thin-layer chromatography) (Chinese). J. of Trad. Chinese Med. & Pharm. Consult. 4 (5), 16-17 (2012). The root of Chinese Eupatorium L. is a herbal TCM drug used in various preparations for the treatment of diphtheria, sore throat, fever, cough, traumatic swelling pain and snake bites. For quality control and to prevent counterfeiting TLC of herbal extracts and the standard euparin on silica gel with n-hexane – chloroform – methanol 20:10:1, detection by spraying with 1 % aluminium chloride in ethanol and heating until the zones are visible in daylight, and under UV 366 nm.

      Classification: 32e
      113 019
      Simultaneous quantification of three bioactive lignans, viz
      R. KHABIYA, D. UPADHYAY, A. SRIVASTAVA, S. ANANDJWALA* (*Department of Natural Products, National Institute of Pharmaceutical Education and Research (NIPER) Ahmedabad, India, drsheetalanand@gmail.com)

      J. Planar Chromatogr. 27, 291-296 (2014). HPTLC of phyllanthin (1), hypophyllanthin (2), and niranthin (3) from Phyllanthus amarus on silica gel with n-hexane - ethyl acetate - glacial acetic acid 30:10:1. Quantitative determination by absorbance measurement at 230 nm. The hRF values for (1) to (3) were 33, 41 and 46, respectively. Linearity was in the range of 200-1200 ng/zone for (1), 200-1000 ng/zone for (2) and 200-1000 ng/zone for (3). The intermediate/interday/intra-day precisions were below 2 % (n=6). The LOD and LOQ were 50 and 200 ng/zone for (1), 100 and 200 ng/zone for (2) and 100 and 200 ng/zone for (3), respectively. Average recoveries for (1) to (3) were 100.1 %, 98.8 % and 100.3 %, respectively.

      Classification: 7, 9