J. Ethnopharmacol. 197, 165-172 (2017). HPTLC of arjunolic acid (1), arjunetin (2), berberin (3), piperine (4), resveratrol (5) and withaferin-A (6) in Ayurvedic formulation on silica gel with ethyl acetate – toluene – formic acid – acetic acid 6:3:5:1 for (1), n-butanol – glacial acetic acid – water 12:3:4 for (2), toluene – ethyl acetate – diethylether 6:3:1 for (3), chloroform – ethyl acetate – formic acid 25:10:1 for (4) and chloroform – methanol 19:1 for (5) and (6). Detection of (1-4) by spraying with anisaldehyde sulfuric acid reagent. Quantitative determination by absorbance measurement at 690 nm for (1) and (2), 366 nm for (3), 330 nm for (4), 307 nm for (5) and 225 nm for (5) and (6). The hRF values for (1-6) were 67, 31, 58, 24, 32 and 37, respectively.

J. Planar Chromatogr. 31, 28-35 (2018). HPTLC fingerprint of twenty Bryophyta species on A) silica gel with ethyl acetate ‒ formic acid ‒ acetic acid ‒_x000D_
water 28:3:3:4 and B) RP-18 with methanol – water – formic acid 14:5:1. Detection by spraying with natural products reagent (1 g of diphenylborinic acid 2-aminoethylester in 100 mL of methanol) followed by PEG reagent (5 g of polyethylene glycol in 100 mL of methanol). Qualitative identification under UV 366 nm.

Phytochemistry 157, 92-102 (2019). HPTLC of monogalactosyldiacylglycerol (1) and digalactosyldiacylglycerol (2) in the leaves of basket willow (Salix viminalis L., Salicaceae, Malpighiales), cabbage (Brassica oleracea L., Brassicaceae, Brassicales), pea (Pisum sativum, Fabaceae, Fabales), roseroot (Rhodiola rosea L., Crassulaceae, Saxifragales), meadow buttercup (R. acris L., Ranunculales), garlic (Allium sativum L., Amaryllidaceae, Asparagales) and Ipomoea tricolor Cav. (Convolvulaceae, Solanales) on silica gel with acetone – benzene – water 91:30:8. Qualitative identification under UV light at 254 and 366 nm. The hRF values were 20-26 for (1) and 63-77 for (2).

J. Chromatogr. A 1530, 197-203 (2017). Evaluation of the phenolic and stigmasterol content and comparison of the antioxidant and antidiabetic activities by HPTLC combined with DPPH* free radical method and α-amylase assay towards ethanol and ethyl acetate extracts of 10 marine macroalgae species (3 chlorophyta, 4 phaeophyta and 3 rhodophyta). HPTLC on silica gel with n-hexane – ethyl acetate – acetic acid 20:9:1 over 80 mm. Detection either by dipping into 0.4 % DPPH* solution followed by storing in the dark for 30 min before evaluation, or by dipping into anisaldehyde – sulfuric acid reagent or neutralized ferric chloride solution (prepared by adding dilute sodium hydroxide to freshly prepared 2 % ferric chloride in methanol until precipitation occurs, followed by filtering), both followed by heating at 100 °C for 10 min. Quantitative evaluation by absorbance measurement at 470 nm for detection of α-amylase inhibition and at 550 nm for stigmasterol. The results showed higher antioxidant activity in the ethyl acetate extracts than in ethanol extracts, and higher amounts of fucoxanthin, stigmasterol and α-amylase inhibitory activities in ethyl acetate extracts. The results proved the relation of higher α-amylase inhibition in ethyl acetate extracts due to the presence of triterpenes and sterols, and no contribution of fucoxanthin to it.

J. Chinese Trad. Patent Med. (Zhongchengyao) 18 (1), 12-13 (1996). TLC on silica with 1) butanol - ethyl acetate - water 4:1:5, 2) butanol - acetic acid - water 3:1:1. Detection by spraying with 10% sulfuric acid and heating at 110°C for 5 min, and with 2% ninhydrin in acetone and heating at 105°C for 5 min. Identification by finger print technique.

CBS 89, 4-7 (2002). HPTLC of devil’s claw extract on lichrospher silica gel with ethyl acetate - ethanol (70 %) 7:3 over 70 mm with chamber saturation. Detection by dipping in anisaldehyde - sulfuric acid reagent followed by heating at 105 °C until colors develop. Determination of bioactivity with DPPH-biotest by spraying with 0.05 % solution of (2,2-di-(4-tert-octylphenol)-1-picrylhydrazyl in methanol. Determination of antibiotic properties with Merck Chrom Biodip test by dipping in bacteria solution and incubation at 26 °C for 20 h, followed by spraying with yellow MTT tetrazolium salt.

J. Chinese Trad. Patent Med. (Zhongchengyao) 25 (12), 970-974 (2004). TLC on silica gel with 1) ethyl acetate - methanol - water 6:1:3, 2) n-butanol - glacial acetic acid - water 4:1:5, 3) chloroform - methanol - water 28:10:1. Detection 1) by spraying with 1 % AICl3 and under UV 365 nm, 2) by spraying with 0.5 % ninhydrin in ethanol and heating at 105 ºC, 3) under UV 365 nm. Identification by fingerprint techniques. Quantitative determination of puerarin by HPLC.

J. Liq. Chrom. Rel. Technol. 27, 2087-2100 (2004). HPTLC of tetrandrine on silica gel with toluene - ethyl acetate - methanol - 28 % ammonia 100:100:50:3. Detection under UV light at 366 nm, and under white light after reaction with iodine (yellowish zones). If the plate is subsequently derivatized with anisaldehyde solution, the alkaloids show a strong blue fluorescence under UV light at 366 nm. Quantitative determination at 210 nm. The calibration curve is linear for 50 - 112.5 ng tetrandine per zone. Also HPTLC of aristolochic acids (AAs) on silica gel with the upper phase of toluene - ethyl acetate - water - formic acid 20:10:1:1 and derivatization with tin(II) chloride (to be prepared freshly: dissolve 1 g tin(II) chloride•2H2O in 1.5 mL 36 % hydrochloric acid diluted with 8 mL water), followed by heating at 100 °C for 1 min and evaluation under UV light at 366 nm. The method allows detection of 1 ppm of AA.