Chinese J. Trad. Patent Med. (Zhongchengyao) 26 (6), 452-455 (2004). TLC of Shangbiao oil extracts on silica gel with 1) n-heptane-ethyl acetate 17:3; 2) n-heptane - ethyl acetate 9:1; 3). Detection 1) by spraying with 2,4-dinitro-phenylhydrazine solution in ethanol; 2) by spraying with 5 % vanillin-H2SO4 solution and heating at 110 ºC for 5 min. Identification by fingerprint techniques. Quantification of menthol and camphor by GC.

Chinese J. Trad. Pat. Med. (Zhongchengyao) 27(1), 84-87 (2005). TLC screening of Sinisan powder extracts, purified with macroporous resins, on silica gel with 1) n-butanol - ammonia - ethanol 7:3:1; 2) chloroform - methanol 7:1. Detection 1) by spraying with 5 % AICI3 in ethanol 2) by spraying with 5 % vanillin - H2SO4 solution. Identification by fingerprint technique. Screening of purification conditions by evaluation of the content of the active principle, and yield of the purified products. Type HP20 macroporous resin has been concluded to be the optimum for active fraction of the recipe in purification efficiency.

J. Planar Chromatogr. 18, 221-223 (2005). HPTLC of horminone on silica gel in a twin-trough chamber with hexane - dioxane 9:1. Absorbance measurement at 271 nm. For fluorescence analysis the plates were dipped in 1 % diphenylboryloxyethylamine in ethyl acetete for 2 s, followed by drying and dipping in a solution of 5 % PEG 8000 in dichloromethane for 2 s. After 15 min fluorescent zones of horminone were scanned at 366/>400 nm. Use of the fluorescence reagent successfully reduced the limits of detection and quantification to 0.75 ng/spot and 1.51 ng/spot, respectively. Linearity range was from 60-300 ng/spot.

J. Chromatogr. A 1077 (2), 202-206 (2005). HPTLC of physcion, chrysophanol, emodin and chrysophanol glycoside in Rheum emodi on RP-18 with methanol - water - formic acid 80:19:1. Quantitative determination by absorbance measurement at 445 nm. The calibration curves were linear in the range of 20-100 ng for physcion, 80-400 ng for chrysophanol and emodin, and 200-1000 ng for chrysophanol glycoside. The method was found to be reproducible and convenient for quantitative analysis of anthraquinone derivatives in the methanolic extract of rhizomes of Rheum emodi collected from three different locations of Western Himalaya, India.

Planta Med. 70, 441-445 (2004). Preparative TLC of tylophoridicine E and F on silica gel with dichloromethane - methanol - ammonia 100:10:1 or 120:10:1. Detection with Dragendorff reagent.

Planta Med. 71, 364-365 (2005). Analytical TLC of surangin B on silica gel with chloroform - toluene 1:1 and toluene - acetone 9:1. Detection under UV light at 254 nm.

J. Chinese Trad. Patent Med. (Zhongchengyao) 27 (7), 854-856 (2005). TLC of chenodeoxycholic acid in Hedan tablets on silica gel with n-hexane - ethyl acetate - acetic acid - methanol 20:25:2:3. Detection by spraying with 10 % H2SO4 in ethanol followed by heating at 105 ºC until the spots are visualized. Quantification of chenodeoxycholic acid by densitometry at 375 nm. Validation of the procedure by investigation of its linearity range (0.47 - 2.33 µg/spot, R = 0.9992); of its repeatability (3.3 %, n = 5); of its precision (3.9 %, n = 5 within plate and 4.7 % n= 5 plate-to-plate); and its standard addition recovery (98.4%, RSD = 2.5 %, n = 5).

Chromatographia 63 (3-4), 209-213 (2006). HPTLC of valerenic acid in Valeriana jatamansi and Valeriana officinalis on silica gel with hexane - ethyl acetate - acetic acid 160:40:1. Detection with anisaldehyde-sulphuric acid reagent. Quantitative determination by absorbance measurement at 700 nm. The calibration curves were linear in the range of 500 ng - 2.5 µg/zone.