Chinese J. of Lishizhen Trad. Med. & Pharm. 22 (4), 860-862 (2011). Fallopia multiflora (Thunb.) Harald, a medicinal herbal drug widely applied in traditional Chinese medicines, is available from different provinces of China. Presentation of fingerprint methods of Fallopia multiflora from different regions by micellar thin-layer chromatography (MTLC) and IR techniques. MTLC of methanol extracts of Fallopia multiflora and emodin standard solution on polyamide layer with 0.4 % cetylpyridinium chloride in acetone – methanol – 1.0 mol/L NaOH 12:4:5 after chamber saturation for 15 min. Detection of the purple zones in daylight. The ratio of the hRf values of the component zones and those of emodin was calculated. In addition, FTIR of the methanolic extracts and emodin standard solution mixed in KBr tablets. The results of the real life samples available from five different regions are given and proved that the method is suitable for the quality control of the drugs.

Chinese J. of Lishizhen Trad. Med. & Pharm. 23 (7), 1822-1823 (2012). Dannangyan compound oral liquid is a herbal TCM preparation for treating cholecystitis and cholelithiasis. For identification, TLC on silica gel 1) for Radix et Rhizoma Rhei, with the upper phase of petroleum ether (30-60 ºC) – ethyl acetate – formic acid 15:5:1, detection at UV 366 nm and by exposure to iodine vapors and viewing in daylight; 2) for Cortex Magnoliae officinalis, with chloroform – benzene – ethyl acetate 10:4:1, detection by spraying with 5 % vanillin in sulfuric acid - ethanol 1:200 followed by heating at 105 °C for 5 min and viewing in daylight.

Chinese J. of Guiyang Med. Coll. 37 (6), 605-607 (2012). Gongyanping capsules are a herbal TCM for treating acute and chronic pelvic inflammatory disease, irregular menstruation, etc. For quality control, TLC on silica gel 1) for Melastoma dodecandrum Lour. and the standard gallic acid, with chloroform – ethyl acetate – formic acid 25:20:4, detection by spraying with 2 % ferric chloride in ethanol and heating mildly, viewing in daylight; 2) for Zanthoxylum nitidum, with n-butanol – glacial acetic acid – water 7:1:2, detection by spraying with 5 % potassium iodobismuthate in water – hydrochloric acid 200:1 and viewing in daylight; 3) for Radix Angelicae Sinensis and the standard ferulic acid, with toluene – chloroform – glacial acetic acid 6:5:1, detection under UV 366 nm; 4) for Ficus simplicissima Lour. and the standard psoralen, with cyclohexane – toluene – ethyl acetate 10:10:3, detection by spraying with 10 % KOH in ethanol and viewing under UV 366 nm.

Chinese J. of Lishizhen Trad. Med. & Pharm. 23 (5), 1180-1181 (2012). Elaeagnus gonyanthes Benth. is a medicinal herb which grows in southern China. The crude drug is used in folk medicine for treating asthma, chronic bronchitis, arthritis, low back pain, traumatic swelling, etc. For identification, TLC of the extracts of different plant parts (leaf, stem) and the standards oleanic acid and ursolic acid on silica gel with cyclohexane – chloroform – ethyl acetate – glacial acetic acid 200:50:80:1, detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 °C, viewing in daylight. Quantification of oleanic acid and ursolic acid by HPLC.

Shanxi J. of Trad. Chinese Med. 33 (12), 1666-1667 (2012). It is highly debatable whether Radix Aconiti Lateralis Preparata is compatible with Trichosanthes kirilowii Maxim when both needed in processing single formulations. A method is presented to investigate the change of the chemical composition of the extracts obtained by separate and mixed decoction of two kinds of drugs. Preparation of the analytes by decocting, separately, of Radix Aconiti Lateralis Preparata (A), Trichosanthes kirilowii Maxim (B) and combined decoction of the two (C), then extracting, separately, with petroleum ether (30—60 °C) producing extracts A1, B1, C1 and with n-butanol producing extracts A2, B2, C2. TLC on silica gel with the upper phase of n-butanol – ethyl acetate – water 4:1:5, detection 1) under UV 366 nm; 2) by spraying with 10 % sulfuric acid in ethanol and viewing under UV 366 nm. By investigation of 5 batches of the drug sample, significant difference was found between the analytes from single drug decoction and those from mixed drug decoction. The unknown ingredients found in the mixed decoction will be further characterized and analyzed. This study provides an experimental base for exploration of the mechanism of the interaction among the ingredients released from the two drugs.

J. Planar Chromatogr. 26, 349-353 (2013). TLC of fluvoxamine maleate (1), paroxetine (2) and sertraline in pharmaceuticals on silica gel with 2-propanol - dichloromethane 7:3. Quantitative determination by absorbance measurement at 366 nm. The hRf of (1), (2), and (3) was 44, 22 and 68, respectively. Linearity was between 200 and 1600 ng/zone for both (1) and (2). Limits of detection and quantitation were 75 and 100 ng/zone for (1), and 40 and 80 ng/zone for (2), respectively. Recovery (by standard addition) was found to be 96 - 107 %.

Chinese J. Mod. Drug Appl. 7 (17), 228-229 (2013). Zhuangyao Zhitong Heji compound oral liquid is a herbal TCM preparation to treat deficiency of the kidney and liver, chills and pain of the waist and knee, wind-cold-dampness arthralgia and low back injury. For quality control, TLC on silica gel with n-hexane – ethyl acetate 9:1, detection under UV 366 nm. Identification of Angelica sinensis, Ligusticum chuanxiong and Saposhnikovia divaricata roots by fingerprint comparison with reference drugs.

Chinese J. of Guide for Trad. Chinese Med. & Pharm. 2 (2), 97-99 (2013). Sanhuang zhiyang chaji liniment is a herbal TCM preparation prescribed for treating skin itching, carbuncle furuncle, acute eczema, impetigo and skin infection. For quality control, TLC on silica gel 1) for Cortex Phellodendri Chinensis and the standard berberine hydrochloride, with toluene – ethyl acetate – isopropanol – methanol – ammonia 12:6:3:1:1, detection by exposure to ammonia vapors and viewing under UV 366 nm; 2) for Radix Scutellariae and the standard baicalin, with ethyl acetate – butanone – methanol – water 5:3:1:1, detection by spaying with 1 % ferric chloride in ethanol and viewing in daylight; 3) for Radix et Rhizoma Rhei and the standard emodin, with petroleum ether (60-90 °C) – ethyl formate – formic acid 15:5:1, detection under UV 366 nm; 4) for Radix Sophorae Flavescentis and the standard matrine, with benzene – propanol – ethyl acetate – ammonia 10:15:20:1, detection by spaying with 5 % potassium iodobismuthate in hydrochloric acid – water 1:200 and viewing in daylight.