J. Planar Chromatogr. 23, 180-183 (2010). TLC of chamomile extracts on silica gel with benzene - ethyl acetate 19:1 or chloroform - methanol - water 40:10:1 in an unsaturated chamber. Detection at 254 and 366 nm. For bioautographic evaluation bioluminescent Bacillus subtilis or Pseudomonas syringae pv. maculicola were used; visualization by dye a reagent was achieved by dipping the plate in an aqueous solution of MTT. Quantitative determination by densitometric scanning at 300 nm (before biological detection) or at 590 nm (after visualization of the bioautogram with MTT).

Ind. J. Pharma. Sci. 72(1), 39-45 (2010). The Ayurvedic Pharmacopoeia of India recognized roots of the three plants Cissampelos pareira, Cyclea peltata, and Stephania japonica (all Menispermaceae) as source for the marketed drug Patha. HPTLC fingerprint analysis of methanolic extracts of roots of all three plants on silica gel with n-butanol - ethyl acetate - formic acid - water 3:5:1:1. Detection under UV 365 nm (crude extract) and 295 nm (total alkaloids). Quantification of the marker berberine by HPLC. The three plants exhibit significantly different physico-chemical features and chromatographic fingerprints. Roots of C. pareira contained the highest concentration of berberine, S. Japonica contained very low amounts and C. peltata no berberine at all.

Chinese J. Ethnomed. Ethnopharm. (1), 55-57 (2011). TLC of components of Baibanding tincture: 1) for Gardenia jasminoides, on silica gel with ethyl acetate – acetone – methanol – water 5:5:1:1, detection by spraying with 10 % sulfuric acid in ethanol and heating at 110 °C until the zones were visualized; 2) for Cuscuta chinensis, on polyamide phase with methanol – glacial acetic acid – water 4:1:5, detection by spraying with AlCl3 solution and evaluation under UV 366 nm; 3) for Malaytea scurfpea fruit on silica gel with n-hexane – ethyl acetate 4:1, detection by spraying with 10 % NaOH in methanol and evaluation under UV 366 nm. Identification by fingerprint comparison with the individual component drug of the preparation.

Chinese J. Ethnomed. Ethnopharm. (23), 61-64 (2010). Optimization of the sample preparation procedure for Herba Saururi chinensis 1) by ultrasonication with methanol for 60 min; 2) by ultrasonication with methanol for 20 min and filtration through neutral alumina column with methanol; 3) by reflux extraction with 80 % methanol for 60 min and extraction with diethyl ether; 4) by ultrasonication with ethanol for 60 min; 5) by ultrasonication with methanol - 25 % hydrochloric acid 4:1 for 60 min and extraction with ethyl acetate. Procedure 5) was best suited. TLC on silica gel 1) with petroleum ether (60-90 °C) – acetone 5:2, and detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 ºC until the zones appear; 2) with toluene – ethyl acetate – formic acid 5:2:1, and detection by spraying with 1 % AlCl3 in ethanol and evaluation under UV 366 nm; 3) with n-hexane – ethyl acetate – formic acid 70:50:8, and detection by spraying with 1 % AlCl3 in ethanol, heating at 105 ºC and evaluation under daylight or under UV 366 nm; 4) with toluene – ethyl acetate – formic acid 5:4:1 with chamber saturation with hydrochloric acid vapor, detection under daylight or UV 366 nm.

Planta Med. 74, 1397-1402 (2008). Analytical and preparative TLC of three steroidal saponins (25R)-spirost-5-ene-3ß, 17alpha-diol (pennogenin), 3-O-{O-alpha-L-rhamnopyranosyl-(1-2)-O-[O-ß-xylopyranosyl-(1-5)-alpha-L-arabinofuranosyl-(1-4)]-ß-D-glucopyranoside} on silica gel with dichloromethane - methanol - water 80:19:1 or 70:29:1. Detection by spraying with phosphomolybdic acid. Additional detection by antifungal bioassays.

Ham. Ex D. Don, Myricaceae. J. Planar Chromatogr. 23, 326-331 (2010). HPTLC of myrecitin and stem bark extracts on silica gel with toluene - ethyl acetate - formic acid - methanol 15:15:3:2. Quantitative determination by absorbance measurement at 268 nm. Linearity was between 0.4-2.0 µg/band. The limits of detection and quantitation were 93 ng and 284 ng/zone. The intra-day and inter-day precision of the method was in the range of 0.14-0.55 %. The recovery of myrecitin at three concentrations was in the range of 98.9-100.1 % and the average recovery was 99.3 %.

Chinese J. of Northwest Pharm. 26 (5), 324-327 (2011). TLC of the extracts of Kelu Oral Liquid on silica gel 1) for Ephedrae herba, with chloroform - methanol - concentrated ammonia 40:7:1, detection by spraying with 5 % ninhydrin in ethanol and heating at 105 °C, identification by fingerprint comparison with ephedrine/pseudoephedrine hydrochloride; 2) for Scutellariae radix, with ethyl acetate - butanone - formic acid - water 5:3:1:1, detection by spraying with 10 % FeCl3 in ethanol and heating at 105 °C, identification by fingerprint comparison with astragaloside IV; 3) for menthol and Tatarian Aster root, with petroleum ether (60-90 °C) - ethyl acetate 9:1, detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 °C, identification by fingerprint comparison menthol and shionone; 4) for Glycyrrhizae radix, with ethyl acetate - formic acid - glacial acetic acid - water 15:1:1:2, detection under UV 365 nm after spraying with 10 % sulfuric acid in ethanol and heating at 105 °C, identification by fingerprint comparison with glycyrrhizic acid ammonium salt; 5) for Loquat leaf, with cyclohexane - chloroform - ethyl acetate - glacial acetic acid 40:10:16:1, detection by spraying with 20 % phosphomolybdic acid in ethanol and heating at 105 °C, identification by fingerprint comparison with ursolic acid; 6) for Fritillaria cirrhosa D. Don, with ethyl acetate - methanol - concentrated ammonia 18:2:1, detection by spraying with 5 % potassium iodobismuthate and 0.2 % sodium nitrite solution, identification by fingerprint comparison with peiminine.

leaves and its geographical variation using HPTLC fingerprint. Trends in Natural Product Research, NRP-2011 abstract No. SNP-NRP-11/069. TLC of ferulic acid in ethanolic extracts of Ricinus communis leaves on silica gel with chloroform - methanol 19:1. Quantitative determination by densitometry at 366 nm. The method was linear in the range of 300-900 ng/band. LOD and LOQ were 4 and 11 ng/zone, respectively. The plant collected from different geographic locations showed variations in the amount of ferulic acid. The content of ferulic acid was 2.87 µg/g in leaves collected from Delhi, which was higher than those from Guwahati and Jhansi.