J. Planar Chromatogr. 24, 116-120 (2011). HPTLC of rosin, rosarin, and rosavin in the roots of Rhodiola rosea L. and Rhodiola sachalinensis Borissova on silica gel, prewashed with methanol, with chloroform - methanol - water 130:70:21 in a twin-trough chamber lined with filter paper and saturated for 20 min, at 21-24 °C and 40-45 % relative humidity. Quantitative determination by densitometry at 250 nm. The calibrations were linear in the range of 100-500 ng/band with correlation coefficients of 0.9999, 0.9992, and 0.9992 for rosin, rosarin, and rosavin, respectively. Recovery of the three compounds was between 97 and 101 %. The LOD and LOQ were 30 and 100 ng/band, respectively, for all three compounds. The hRf values for rosin, rosarin, and rosavin were 58, 45, and 42, respectively. Intra-day variation, as %RSD, was 2.7 % for rosin, 1.7 % for rosarin, and 2.8 % for rosavin, with standard errors of 0.3 %, 0.2 %, and 0.3 %, respectively. Inter-day variation, as % RSD, was 4.8 % for rosin, 4.2 % for rosarin, and 3.9 % for rosavin, with standard errors of 0.5 %, 0.5 %, and 0.4 %, respectively.

J. AOAC Int. 94, 1422-1426 (2011). HPTLC of eleutherosides B, E, and E1 in the fruits and leaves of E. senticosus and five other species of Eleutherococcus species after extraction with SPE, on silica gel in the horizontal chamber with saturation for 20 min, with a two-step development with 1) chloroform - methanol - water 70:30:4 and 2) chloroform - methanol - toluene - ammonium hydroxide 9:6:3:2. Detection by immersion in Liebermann-Burchard reagent for 1 s and heating at 110 °C for 10 min. The hRf values for the eleutherosides E, B, and E1 were 43, 53, and 66, respectively.

26th IPGA Annual conference, 12 (2010). TLC of berberine in plant extracts and methanolic solutions of a commercially available herbal formulation on silica gel with n-butanol - acetic acid - water 14:3:4. Densitometric evaluation at 435 nm. The method was found to be linear in the range of 8.1-26.9 ng/band. The proposed method was applied to the analysis of different formulations using berberine as chemical marker.

J. Strait Pharm. 22 (12), 59-60 (2010). TLC of the extracts of the title traditional Chinese medicine 1) for red Ginseng, on silica gel with benzene - ethyl acetate - formic acid 20:16:3, detection by spraying with 3 % FeCl3 in ethanol and viewing under daylight; 2) for Chinese Taxillus twig, on silica gel with water-saturated toluene - ethyl acetate - formic acid 5:4:1, detection by spraying with 5 % AlCl3 in ethanol; 3) for Hawthorn, on silica gel with toluene - ethyl acetate - glacial acetic acid 24:8:1, detection by spraying with 3-10 % sulfuric acid in ethanol and heating at 105 °C until the zones were detected; 4) for Alisma orientale, on silica gel with toluene - ethyl acetate - formic acid 14:7:2, detection by spraying with acetic acid - concentrated sulfuric acid - ethanol 1:1:1, heating at 105 °C until the zones were detected and viewing under daylight. Identification by comparison with the standards of the individual drug.

Planta Med. 77, 742-748 (2011). Preparative TLC of two monoterpenes, two sesquiterpenes, twenty-two flavonoids, fourteen lignans, and thirty-two other compounds on silica gel with petroleum ether - ethyl acetate 5:2 and 2:1 and chloroforrm - methanol 100:1, 10:1 and 20:1.

Chinese J. of Veterinary Drugs 46 (5), 29-31 (2012). TCM is more and more important in veterinary medicine. It has been found that some medicines were illegally mixed with certain chemicals harmful to the animal body. Rapid screening of the additives illegally mixed in Astragalus polysaccharides injection by TLC on silica gel with 1) chloroform – methanol – acetone – ammonia 9:2:1:0.05 and 2) ethyl acetate – ethanol 1:1. Detection 1) under UV 254 nm; 2) by spraying with 5 % potassium iodobismuthate solution and evaluation in daylight; 3) by exposure to iodine vapor for a few minutes and evaluation under UV 254 nm. Identification of lincomycin, enrofloxacin, novalgin, 4-acetamino phenol, moroxydine hydrochloride, and ribavirin by comparison of the fingerprint with individual standards, with detection limits of 2.0, 0.5, 1.0, 0.5, 1.0, and 4.0 mg/mL, respectively. The method was applied to 25 batches of market samples and the results were compatible with those obtained by HPLC.

Modern J. of Integrated Trad. Chinese & Western Med. 21 (16), 1786-1788 (2012). Ruoshiming oral liquid is a herbal TCM preparation for the treatment of children’s optic nerve hypoplasia, myopia, hyperopia, and astigmatism. For identification, TLC of the extracts of the preparations on silica gel 1) for Radix Rehmanniae Preparata, with benzene – diethyl ether 5:2, detection by spraying with 2 % ferric chloride – 2 % potassium ferricyanide 1:1 and viewing in daylight; 2) for Pericarpium Citri Reticulatae, first with ethyl acetate – methanol – water 100:17:13 and then with the upper phase of toluene – ethyl acetate – formic acid – water 20:10:1:1, detection by spraying with 3 % aluminium chloride in ethanol and viewing at UV 366 nm; 3) for Rhizoma Acori Tatarinowii, with chloroform – diethyl ether 8:1, detection by viewing in daylight; 4) for Polygonatum sibiricum, with benzene – ethyl acetate – formic acid 50:40:1, detection by spraying with potassium iodobismuthate – HCl – water 10:1:200 and viewing in daylight; 5) for Fructus Lycii, developed with benzene – diethyl ether 2:1, detection by viewing in UV 366 nm.

of China Pharm. 26 (1), 50-53 (2012) Astragalus, as traditional Chinese medicinal herbal crude drug, is the dried root of Astragalus membranaceus (Fisch.) Bge. Var. Monghoicus (Bge.) Hisao or Astragalus membranaceus (Fisch.) Bge. It is specially effective for nourishing vitality, invigorating splenic yang, inducing diuresis for removing edema and promoting tissue regeneration. TLC of the extracts of the crude drugs on silica gel 1) for amino acids (standards arginine, alanine, proline), with carbolic acid – water 5:3 (carbolic acid was double distilled just before mixing with water), detection by spraying with 0.5 % ninhydrin in ethanol and heating at 105 ºC until the zones are visible under daylight; 2) for flavonoids (standards formononetin, calycosin glucoside, calycosin), with chloroform – methanol 10:1, detection by exposure to ammonia vapors for a few minutes and viewing under UV 254 nm.