Chinese J. Trad. Pat. Med. (Zhongchengyao) 27(1), 34-37 (2005). TLC on silica gel with 1) benzene - acetone - ethyl acetate - ammonia water 10:15:20:1; 2) ethyl acetate - butanone - formic acid - water 5:3:1:1; 3) n-butanol - glacial acetic acid - water 7:1:2. Detection 1) by spraying with potassium iodobismuthate solution; 2) by spraying with 2 % FeCI3 in ethanol; 3) by spraying with vanillin - H2SO4 solution and heating; 4) under UV light. Identification by fingerprint technique. Quantification of geniposide by HPLC. The results for ten real life samples are given.

J. Chinese Trad. Patent Med. (Zhongchengyao) 27(5), 535-538 (2005). TLC of the extracts on silica gel with 1) benzene - ethyl acetate - formic acid 15:2:1; 2) n-hexane - ethyl acetate - formic acid 60:20:1; 3) chloroform - methanol - ammonia 40:10:1; 4) chloroform - methanol - water 13:7:2. Detection 1) under UV 365 nm; 2) by spraying with 3 % ninhydrin solution followed by heating at 105 ºC until the spots are visualized; 3) by spraying with 10 % H2SO4 solution in ethanol followed by heating until the spots are visualized. Identification by fingerprint technique. Quantification of emodin by densitometry at 445 nm. Validation of the method by investigation of its linearity range (0.1 µg - 1.0 µg, r = 0.998); precision (RSD = 1.05 % n = 6); reproducibility of six time assay towards the same sample (RSD = 1.24 %); and standard addition recovery (96.7 %, RSD = 1.75 %, n = 6). The results for three batches of real life sample are given.

Planta Med. 70, 877-880 (2004). Preparative TLC of a new seco-abietane-type diterpenoid 13S-hydroxy-9-oxo-9,10-seco-abiet-8(14)-en-18,10alpha-olide, pinoresinol and reduction products on silica gel with n-hexane - ethyl acetate - methanol 25:25:1, chloroform - methanol 9:1, and 19:1. Detection under UV light at 254 nm.

Planta Med. 71, 194-196 (2005). Analytical TLC of glaucopines A and B on silica gel with dichloromethane - methanol 19:1. Detection by spraying with 50 % sulfuric acid.

Planta Med. 71, 871-876 (2005). Preparative TLC of 3,5-dicaffeoylquinic acid and 1,3-dicaffeoyl-epi-quinic acid and 6 known dicaffeoylquinic acid derivatives on RP18 with 40 % aqueous methanol. Detection under UV at 254 nm.

J. Pharm. Biomed. Anal 37 (5), 971-977 (2005). HPTLC of colchicine in Colchicum autumnale (meadow saffron) extracts, on silica gel with chloroform - acetone - diethyl amine 5:4:1. Quantification in absorbance mode at 350 nm. The peaks were optimized in area and shape by varying 4 scanning parameters (slit width and height, number of measurements and scanning speed). Calibration range of pure colchicine was 50-600 ng/mL. This method can be used in pharmaceutical industry for quick and accurate quantitative determination of colchicine because it eliminates the inteferences given by other bioactive or degradation compounds. The method was validated regarding linearity, accuracy, fidelity, and sensitivity.

J. Planar Chromatogr. 19, 348-354 (2006). HPTLC of puerarin, daidzein, daidzin, and 3’-methoxypuerarin on silica gel, pre-washed with methanol, in an unsaturated twin-trough chamber with chloroform - methanol - ethyl acetate - water 16.2:18.8:52:3. Quantitative determination by absorbance measurement at 254 nm. The relative standard deviation of Rf values, retention times and peak area percentages all meet the national standards.

J. Chinese Trad. Patent Med. (Zhongchengyao), 27 (8), 903 - 905 (2005). TLC of Tangniaoling tablets on silica gel with 1) chloroform - methanol 20:1; 2) chloroform - methanol 10:1. Detection 1) by spraying with 5 % vanillin in H2SO4 solution and heating at 105°C. Identification by fingerprint techniques. Quantification of puerarin by HPLC.