Tianjin J. of Trad. Chinese Med. & Pharm. 28 (2), 164-166 (2011). TLC of the extracts of the traditional Mongolian medicine on silica gel 1) for artificial cow-bezoar, with isooctane - ethyl acetate - glacial acetic acid 6:3:2, detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 °C until the zones were detected under UV 365 nm, identification by comparison with the fingerprint of the individual drug components and cholic acid as reference; 2) for Coptidis rhizoma, with benzene - ethyl acetate - isopropanol - methanol - water 20:10:5:5:1, detection under UV 365 nm after exposure to ammonia vapour, identification by comparison of the fingerprint with the individual drug components and berberine hydrochloride as reference; 3) for light yellow Sophora root, with ethyl acetate - propanone - benzene - ammonia water 20:15:10:1, detection by spraying with 5 % potassium iodobismuthate solution, identification by comparison of the fingerprint with the individual drug components and with matrine as reference.

PHCOGJ 2(11), 374-380 (2010). Root bark of Alangium salvifolium (Linn.f.) Wang is a reputed drug mentioned in the ancient books of Ayurveda and Siddha for the treatment of epilepsy, jaundice, hepatitis etc. TLC of ethanolic, chloroform, and ethyl acetate extracts of the root bark of Alangium salvifolium on silica gel with toluene - ethyl acetate - diethylamine 10:7:1. Evaluation under UV 254 nm and 366 nm.

Brazilian Journal of Pharmacognosy 21, 818-823 (2011). This review describes recent advances in HPTLC automatization as a useful tool for the analysis of complex mixtures of natural products. The author also compares HPTLC with TLC and HPLC. The review provides a general perspective for HPTLC fingerprint approach for the analytical determination of botanicals.

Indian drugs 48 (02), 43-47 (2011). TLC of curcumin and 3-acetyl-11-keto-beta-boswellic acid on silica gel with chloroform - methanol 37:3 for curcumin and n-hexane - ethyl acetate 1:1 for boswellic acid derivatives. The hRf value of 3-acetyl-11-keto-beta-boswellic acid was 24 and of curcumin 59. Densitometric evaluation at 430 nm for curcumin and 254 nm for the acid. The method was linear in the range of 100-500 ng/band for curcumin and 1500-4000 ng/band for 3-acetyl-11-keto-beta-boswellic acid.

J. Planar Chromatogr. 24, 77-81 (2011). HPTLC of belleric acid on silica gel, prewashed with methanol, with toluene - ethyl acetate - methanol - formic acid 15:15:7:1 in a saturated chamber at 22 °C and 65 % relative humidity. Quantitative determination by absorbance measurement at 205 nm. Average recovery was 98.7-100.9 %. Linearity was between 250 and 1250 ng/zone. Repeatability and intermediate precision (%RSD) were 1.2 and 1.5 %, respectively. LOD and LOQ were 49 and 148 ng/zone, respectively. The hRf value of belleric acid was 35.

J. Planar Chromatogr. 24, 470-474 (2011). TLC of methanolic seed extracts with harmine, harmaline, and galanthamine as standards on silica gel with ethyl acetate - methanol - ammonia 20:3:1. Detection by inspection under UV 366 nm and by spraying with Dragendorff’s and vanillin-sulfuric acid reagent as well as by bioautography assay. For the bioautographic assay, AChE was dissolved in 150 mL of 0.05 M TRIS/hydrochloric acid buffer at pH 7.8. 150 mg bovine serum albumin was added for stabilization.The plates were dipped in the enzyme stock solution and incubated in a humid chamber at 37 °C for 20 min. For detection of the enzyme, solutions of 1-naphthyl acetate in ethanol and of Fast Blue B salt in water were prepared and mixed immediately before spraying the plate. The limits of AChE inhibitive activity were 10 ng/zone for all.

J. Strait Pharm. 24 (2), 65-66 (2012). Yishen Antai ointment is a herbal TCM recipe specialized for tonifying the kidney and miscarriage prevention of pregnant woman with marked clinical curative effect. Description of a procedure for the analysis of Atractylodes macrocephala Koidz, one of the key components of the preparation. TLC on silica gel with petroleum ether (60-90 ºC) – ethyl acetate 50:1, detection by spraying with 5 % vanillin in a solution of ethanol – sulfuric acid 100:1 and heating at 105 °C until the zones were visualized.

J. of China Pharm. 19 (22), 41-43 (2010). Yanreqing capsules are a herbal TCM preparation specially effective for heat clearing and detoxifying, and prescribed clinically to cure bronchitis, pneumonia, acute tonsillitis, urinary system infections and biliary tract infections. TLC of the extracts of the medicine on silica gel 1) for Baical Skullcap, with ethyl acetate – butanone – formic acid – water 5:3:1:1, detection by spraying with 2 % ferric chloride in ethanol and viewing in daylight; 2) for Cape jasmine, with ethyl acetate – acetone – formic acid – water 5:5:1:1, detection by spraying with 10 % sulfuric acid in ethanol and heating at 110 °C until the zones are visible.