J. Liq. Chromatogr. Relat. Technol. 43, 414-423 (2020). HPTLC fingerprint of Ganoderma lucidum fruiting body on silica gel with toluene - tetrahydrofuran - acetic acid 70:30:1. Detection by dipping into a solution of 10% sulfuric acid in methanol, followed by heating at 100 ºC for 3 min. Qualitative determination under UV light at 254 and 366 nm. The hRF values for ganoderic acids D, B, A, G and C2 were in the zone between 10 and 50. This zone was used for quantification of total triterpene acids by absorbance measurement at 260 nm. Linearity of each of the main peaks between hRF 10 to 50 was determined. The linear range of ganoderic acid A was between 200 and 500 ng/zone.  The study proposes a new method for evaluation, based on “comprehensive high-performance thin layer chromatography (HPTLC) fingerprinting.” Instead of several different methods using different chromatographic techniques, a single HPTLC analysis for identification of Ganoderma lucidum fruiting body with a test for adulteration and quantitative determination of the content of total triterpene acids is proposed. As an alternative to the current tests in the  USP monograph on G. lucidum fruiting body this HPTLC method is a single, low-cost test, which eliminates the UHPLC assay of total triterpene acids.

J. Liq. Chromatogr. Relat. Technol. 43, 388-393 (2020). HPTLC of carpaine in the leaves of Carica papaya on silica gel with chloroform - methanol 7:3. Detection by dipping into Dragendorff’s reagent. Quantitative determination by absorbance measurement at 510 nm. The hR_F value for carpaine was 59. Linearity was between 0.4 and 1.2 µg/zone. Intermediate precisions were below 2 % (n=3). The LOD and LOQ were 11 and 33 ng, respectively. Recovery was 101.6 %.

J. Planar Chromatogr. 33, 179-189 (2020). HPTLC of plumbagin in the roots of Plumbago zeylanica on silica gel with toluene - ethyl acetate 3:1. Quantitative determination by absorbance measurement at 270 nm. The hR_F value for plumbagin was 64. Linearity was between 400 and 1200 ng/mL. Intermediate precisions were below 1 % (n=3). The LOD and LOQ were 1 and 3 ng/zone. Average recovery was found to be in the range of 99.8-100.2 %.

J. Planar Chromatogr. 33, 191-201 (2020). HPTLC of rutin in the leaves of Phoenix sylvestris on silica gel with chloroform - methanol - formic acid 10:40:1. Quantitative determination by absorbance measurement at 265 nm. The hR_F value for rutin was 71. Linearity was between 200 and 1000 ng/zone. Intermediate precisions were below 2 % (n=5). The LOD and LOQ were 125 and 205 ng/zone. Average recovery was found to be in the range of 99.0-100.0 %.

J. Planar Chromatogr. 33, 33-41 (2020). HPTLC fingerprint of the kernel and seed of Allanblackia parviflora fruit (extracted with methanol - water 4:1) on silica gel with methanol - water - ethyl acetate 33:27:200. Detection by spraying with vanillic acid - sulfuric acid reagent (1 g of vanillic acid in 100 mL of 96 % ethanol, followed by the dropwise addition of 2 mL concentrated sulfuric acid). Different formulations of the derivatization reagent were investigated and the use of vanillic acid instead of the more frequently used vanillin provided a better result. Qualitative determination under UV 254 and 366 nm. Consistent hR_F values of the main zones under UV 254 nm were 39, 35 and 12. For the derivatized plate, consistent hR_F values of the main zones were 39, 34, 31 and 22.

J. of Chromatogr. Sci. 57 (8), 688 - 696 (2019). Tephrosia purpurea (L.) Pers., commonly known as “wild indigo”, is used in TCM to treat liver, spleen and kidney disorders. To investigate the seasonal effect on the quantity of its phytoconstituents lupeol, β-sitosterol and rotenone, analysis of the extracts from plant material collected in different seasons by HPTLC on silica gel with toluene - ethyl acetate - formic acid 9:1:1. The zones due to  β-sitosterol, rotenone and lupeol were observed at hRF 38, 45 and 52, respectively. Validation of the method in terms of precision, repeatability, specificity, sensitivity, linearity and robustness. The quantitative results obtained with this method showed that the content of these phytoconstituents varies from season to season. It was found that T. purpurea should not be collected in winter season for the preparation of therapeutic formulations because of the high content of rotenone, which is responsible for Parkinson’s disease and associated with heart failure, fatty liver and liver necrosis.

J. of Modern Trad. Chinese Med. 20 (7), 821-824 (2018). Mulusin, a class of isoprene flavonoids extracted from Mori Cortex, has anti-tumor, anti-inflammatory, hypoglycemic, hypolipidemic, analgesic, anti-spasm, and cholinesterase restricting activity. For quality control, TLC of mulusin on silica gel with petroleum ether - dichloromethane - ethyl acetate 15:8:10 at 25 ± 0.3 ˚C with chamber saturation for 30 min. Detection at UV 254 nm. The hRF of mulusin standard was 43. Quantitative absorbtion measurement of morusin by densitometry at 273 nm. Linearity was in the range of 200 - 1100 ng/zone (r=0.999), precision on one plate was RSD = 1.21 % (n=6) and on plate-to-plate RSD = 2.30 % (n=6). Recovery from standard sample addition was 99.2 % (RSD = 1.51, n = 6). The LOD was 20 ng/zone and LOQ 40 ng/zone.

J. Chromatogr. Sci. 57 (5), 411-417 (2019). The high polarity of the has made it difficult to quantify the alkaloids. This study was designed to develop and validate a thin-layer chromatography (TLC) densitometric-based method using high-performance thin-layer chromatography for quantification of berberine. HPTLC of the protoberberine alkaloids in Coptis teeta rhizome on silica gel aluminium foil with butanol - ethyl acetate - formic acid - water 3:5:1:1. Quantitative determination by absorbance measurement at 351 nm. The hRf of berberine was 70. The linearity obtained graphically with a correlation coefficient of 0.997 was in the concentration range of 90-210 ng/band.LOD and LOQ were 30 and 70 ng/band, respectively. The berberine concentration in the methanol extract of C. teeta was 30.9 ± 0.6 mg in 100 mg of the crude drug. The method developed here in can be implemented in the analysis and routine quality control of herbal materials and formulations containing C. teeta and berberine.