(Chinese). Chinese J. Ethnomed. Ethnopharm. 16, 16-18 (2013). Buqi Shengxie Tie plaster is a TCM patch for the treatment of anemia and white blood cell reduction, etc. Ferulic acid is one of the effective ingredients known. To investigate the skin permeability of ferulic acid in the patches, the patches were applied for different durations to three selected patients. Preparation of test sample solutions by extraction from the used patches. TLC of these test samples on silica gel. Quantitative determination of ferulic acid by absorbance measurement at 370 nm. The linearity was between 0.6 and 2.6 μg/zone and the inter-day precision %RSD was 0.5 % (n = 5). Calibration of the measured contents of ferulic acid in the used patches indicated that the skin permeability of ferulic acid was significant and increased proportionally with the duration of application to the body.

J. Planar Chromatogr. 28, 256-261 (2015). HPTLC of picroside-I (1) and picroside-II (2) in Picrorhiza kurroa on silica gel with ethyl acetate - methanol - acetic acid 80:10:1. Detection by dipping into anisaldehyde - sulfuric acid, followed by heating at 120 °C for 5 min. Quantitative fluorescence measurement at UV 366 nm. The hRF values for (1) and (2) were 57 and 66, respectively. Linearity was in the range of 200-4000 ng/zone. LOD and LOQ were 16 and 47 ng/zone for (1) and 16-48 ng/zone for (2). The intermediate precision was below 11 % (n=3).

J. Ethnopharmacol. 174, 178-186 (2015). HPTLC of magnolol (1) and honokiol (2) in the cortex of Magnolia officinalis and aristolochic acid I (3) and II (4) in the radix of Aristolochia baetica on silica gel with methanol - ethyl acetate - toluene 1:2:30 for (1) and formic acid - water - ethyl acetate - toluene 1:1:10:20 for (2). Detection of (1) and (2) by spraying with vanillin reagent, followed by heating at 110 °C for 5 min. Detection of (3) and (4) by spraying with stannous chloride 100 g/L in diluted hydrochloric acid, followed by heating at 100 °C for 1 min. Identification under UV light at 365 nm for (3) and (4). The hRF values for (1) to (4) were 40, 50, 46 and 54, respectively.

Rev. Bras. Farmacogn. 26, 1-14 (2016). HPTLC fingerprinting of three varieties of Marantodes pumilum on silica gel with chloroform - methanol 9:1. Detection by dipping into a mixture of p-anisaldehyde and sulfuric acid reagent, followed by heating at 100 ºC for 10 min. Qualitative evaluation under UV light at 254 and 366 nm. M. pumilum var. pumila leaves differ from the other two varieties based on the presence of an orange band at hRF 22 or 56. The stem extract of M. pumilum var. alata had the least intense orange band at either hRF 22 or 56. A peak at hRf 22 appeared as a major compound of the stem of M. pumilum varieties in the order of var. lanceolata > var. pumila > var. alata, and can be used for quality control and identification of different parts of the plant materials.

J. Ethnopharmacol. 189, 186-193 (2016). HPTLC fingerprinting of salannin in the leaves of neem (Azadirachta indica A. Juss.; Meliaceae) on silica gel with ethyl acetate – dichloromethane – acetic acid – formic acid – water 100:25:10:10:11. The plates were allowed to dry at 100 °C for 5 min and then derivatized with Natural Product reagent (NPR) (1 g diphenyl borinic acid amino ethyl ester in 200 mL of ethyl acetate), followed by heating at 100 °C for 2–3 min and then dipped into anisaldehyde-sulfuric acid reagent (1 mL p-anisaldehyde, 10 mL sulfuric acid, 20 mL acetic acid in 170 mL methanol) and dried at 120 °C for 5 min. Qualitative identification under UV light at 254 or 366 nm.

J. Chromatogr. Sci. 54 (1), 64-69 (2016). Micro-TLC of 11 species of the Mentha genus and two finished pharmaceutical products in two-dimensional mode in normal (NP) and reversed-phase (RP) systems on cyano phase with non-aqueous eluents for NP-TLC and with mixtures of acetonitrile with water or methanol with water for RP-TLC. Optimization of one-dimensional systems was performed by determining RM values vs. composition of mobile phase dependencies for standards occurring in various Mentha sp. On the basis of these dependencies the most selective chromatographic systems for each run were chosen. Then most selective eluents were applied to optimize two-dimensional systems by creating Rf in NP systems vs. Rf in RP systems correlations. The best two-dimensional systems were chosen on the basis of R(2) values for Rf vs. Rf correlations (the lowest values of R(2) coefficients). Two-dimensional micro-TLC was used to separate phenolic compounds of the examined plant materials.

J. Ethnopharmacol. 204, 26-35 (2017). HPTLC of quercitrin (1), isoquercitrin (2), quercitrin-3-O-β-D-xylopyranosyl-α-L-rhamnopyranoside (3) on silica gel with ethyl acetate – formic acid – acetic acid – water 100:11:11:26. Detection by spraying with NEU`s reagent (diphenylborinic acid 2-aminoethylester, natural product reagent), followed by drying at 110 °C for 2 min. Quantitative determination by absorbance measurement at 265 nm. LOD and LOQ were 31 ng/zone and 75 ng/zone for (2). Recovery was between 99.8 and 101.1 % for (2). Intermediate precision was <2 % (n=3).

J. Chromatogr. A 1526, 137-150 (2017). HPTLC of physalins from crude extracts of Chinese lantern (Physalis alkekengi L.) on silica gel with ethyl acetate – toluene – formic acid 35:15:1. Densitometric screening of physalins in absorption and in fluorescence mode after post-chromatographic derivatization with sulfuric acid reagent. Identification of the physalin L standard and its impurity, 2,3,25,27-tetrahydrophysalin A. Applying two successive plate pre-developments with methanol – formic acid 9:1 and methanol to avoid strong ion suppression caused by the developing solvent additive (formic acid), and to improve the sensitivity of HPTLC-MS/MS method combined with a slightly modified developing solvent ethyl acetate – toluene – formic acid 30:20:1. Non-targeted characterization of physalins from the same chromatographic zone and determination of physalin types by simultaneous hyphenation of HPTLC with a triple quadrupole and an ion trap mass analyzer. Demonstration of the performance of the HPTLC-densitometric and HPTLC–MS/MS methods for the analysis of physalins from aqueous reflux extracts. Observation of variations in physalin profiles and abundances in different parts of P. alkekengi harvested at different stages of maturity, showed that the husks are the most suitable plant part for P. alkekengi quality control.