Chinese J. of Northern Pharmacy 11(5), 14-16 (2014). Zhuanggu Tongluo Wan pills are a TCM preparation for the treatment of degenerative joint disease and osteoporosis. For quality control, TLC on silica gel (1) for Epimedium brevicornum Maxim. and the standard icariin, with ethyl acetate – acetone – methanol – water 20:2:3:2, detection at UV 254 nm; (2) for Paeonia veitchii and the standard paeoniflorin, with chloroform - ethyl acetate – methanol – formic acid 200:25:50:1, detection by spraying with 5 % vanillin in sulfuric acid – ethanol 1:4 and heating at 105 °C until the zones are visible; (3) for aconitine, with chloroform - ethyl acetate 1:1 after exposure to ammonia vapor, detection by spraying with a solution of potassium iodobismuthate – hydrochloric acid – water 25:1:500 and evaluation in daylight.

J. Planar Chromatogr. 28, 300-306 (2015). HPTLC of ellagic acid (1), gallic acid (2) and methyl gallate (3) in gall of Quercus infectoria on silica gel with toluene - ethyl acetate - formic acid 12:9:4. Quantitative determination by absorbance measurement at 300 nm. The hRF values for (1) to (3) were 19, 29 and 46, respectively. Linearity was in the range of 590-1181 ng/zone for (1), 691-2114 ng/zone for (2) and 693-2078 ng/zone for (3). LOD and LOQ were 15 and 51 ng/zone for (1), 243 and 812 ng/zone for (2) and 155 and 516 ng/zone for (3), respectively. The intermediate precision was 3.5 % (n=3). Recoveries ranged between 96 and 102 % for (1), 97 and 105 % for (2) and 95 and 97 % for (3).

J. Ethnopharmacol. 175, 131-137 (2015). HPTLC of 11-hydroxymustakone (1), N-formylannonain (2) and yangambin (3) in Tinospora cordifolia on silica gel with chloroform - ethyl acetate - methanol 130:80:3. Detection by dipping into anisaldehyde sulfuric acid reagent, followed by heating at 100 °C for 5 min. Quantitative determination by absorbance measurement at 366 nm. LOD and LOQ were 75 and 225 ng/zone for (1), 150 and 450 ng/zone for (2) and 30 and 90 ng/zone for (3), respectively. The intermediate precision was below 3 % (n=3). Recovery for (1) to (3) was in the range of 99-108 %.

Journal of Guiyang College of Traditional Chinese Medicine 36 (4), 27-29 (2014). Jianpi Wenwei Jiaonang capsules are a herbal TCM used in the treatment of gastritis. For quality control, TLC for (1) Codonopsis pilosula (Franch.) Nannf., on silica gel with n-butanol – glacial acetic acid – water 14:2:1, detection by spraying with 10 % sulfuric acid in ethanol and heating at 100 °C until the zones are clearly visualized, viewing in daylight; (2) Atractylodes macrocephala Koidz., on silica gel with petroleum ether (60-90 °C) – ethyl acetate 10:1, detection by spraying with 5 % dimethylaminobenzaldehyde in sulfuric acid – ethanol 1:10 and heating until the zones are visualized in daylight; (3) Poria cocos (Schw.) Wolf, on silica gel with toluene – ethyl acetate – formic acid 40:10:1, detection under UV 366 nm; (4) Corydalis yanhusuo W. T. Wang ex Z. Y. Su et C. Y., on silica gel with toluene – acetone 9:2, detection under UV 366 nm; (5) for Salvia miltiorrhiza Bge., on silica gel with toluene – chloroform – ethyl acetate – methanol - formic acid 4:6:8:1:4, detection under UV 254 nm.

J. of Chromatogr. Sci. 52 (1), 5-11 (2014). Presentation of a method for the simultaneous determination of diacerein in the presence of rhein, the active metabolite and hydrolytic degradation product of diacerein, and emodin, the diacerein impurity, in bulk powder and different pharmaceutical formulations. TLC on silica gel with hexane – ethyl acetate – acetic acid 75:50:1, detection and quantification by densitometry at 230 nm. The hRf values were 12, 44 and 60 for diacerein, rhein and emodin, respectively. The linearity ranges were 0.5–10 µg/band for diacerein and rhein, and 0.5–7 µg/band for emodin. Comparison with a HPLC method showed no significant differences.

J. Planar Chromatogr. 29, 330-335 (2016). HPTLC of wedelactone in Eclipta alba, Wedelia calendulacea and Wedelia trilobata on silica gel with toluene – chloroform – ethanol – formic acid 10:8:2:1. Quantitative determination by absorbance measurement at 351 nm. The hRF value for wedelactone was 29. Linearity was between 80 and 280 ng/zone. The intermediate precision was below 2.5 % (n=3). The LOD and LOQ was 0.36 and 1.09 ng/zone, respectively. Recoveries were between 99.4 and 100.3 %.

J. Planar Chromatogr. 30, 264-270 (2017). HPTLC of quercetin (1), apigenin (2) and kaempferol (3) in the stem bark of C. macleodii, C. rothi and C. dichotoma on silica gel with toluene ‒ ethyl acetate ‒ glacial acetic acid ‒ formic acid 10:10:1:2. Quantitative determination at UV 254 nm. The hRF values for (1) to (3) were 64, 66 and 71. LOD and LOQ were 9 and 29 ng/zone for (1), 30 and 92 ng/zone for (2) and 57 and 173 ng/zone for (3), respectively. The intermediate precision (n=6) was below 2 % for (1) and (2).

(Xi Xian) by a validated high-performance thin-layer chromatographic method. J. Planar Chromatogr. 30, 516-520 (2017). HPTLC of chlorogenic acid (1) and caffeic acid (2) in the leaves of Siegesbeckia orientalis on silica gel with toluene ‒ ethyl acetate ‒ formic acid ‒ methanol 30:30:8:3. Quantitative determination by absorbance measurement at 366 nm. The hRF values for (1) and (2) were 19 and 83, respectively. Linearity was between 200 and 600 ng for (1) and (2). LOD and LOQ were 20 and 40 ng/zone for (1) and 59 and 120 ng/zone for (2), respectively. The intermediate/interday/intra-day precisions were below 3 % (n=3). Recovery was 99.0 %.