J. Chromatogr. Sci. 53 (5), 816-823 (2015). HPTLC of ursolic acid, betulinic acid, stigmasterol and lupeol in Shankhpushpi botanicals on silica gel in a twin-trough glass chamber with petroleum ether - ethyl acetate - toluene 7:2:1, detection by spraying with anisaldehyde reagent. Densitometric evaluation at 580 nm. The hRF value of ursolic acid was 21, of betulinic acid 29, of stigmasterol 33 and of lupeol 50. Linearity was between 100-600 ng/zone for all substances. Results of the analysis of real life samples: 134.2 mg/g and 146.1 mg/g ursolic acid in Clitorea ternatea (CT) and Canscora decussata (CD); 110.6 mg/g betulinic acid in Evolvulus alsinoides (EA); 92.8 mg/g, 154.9 mg/g, 31.9 mg/g and 39.2 mg/g stigmasterol in EA, Convolvulus pluricaulis, CT and CD; 30.1 mg/g lupeol in CT.

Chinese J. of Inform. on TCM 20 (7), 59-61 (2013). Shengxinfa Jiaonang capsule is a herbal TCM preparation for treatment of hair loss caused by blood deficiency and kidney essence deficiency, scalp pruritus, alopecia areata, and postpartum alopecia. For quality control, TLC on silica gel (1) for Fallopia multiflora (Thunb.) Harald, with toluene – ethyl acetate – formic acid 15:2:1, detection in daylight; (2) for Psoralea corylifolia Linn., with n-hexane – ethyl acetate 4:1, detection by spraying with 10 KOH in methanol and viewing under UV 366 nm; (3) for Gastrodia elata Bl., with chloroform – ethyl acetate 1:1, detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 °C until the zones are visible. Quantification of 2,3,5,4'-tetrahydroxy stilbene-2-Ο-β-D-glucoside by HPLC.

J. Planar Chromatogr. 29, 336-340 (2016). HPTLC of isoalantolactone (1), germacranolide (2), β-sitosterol (3), and lupeol (4) in the roots of Inula cappa on silica gel with toluene ‒ methanol 47:3. Detection by dipping into anisaldehyde reagent followed by heating at 105 ºC until colored bands appeared. Quantitative determination by absorbance measurement at 525 nm. Linearity ranged from 10-70 μg/mL for (1), 10-60 μg/mL for (2), 8-48 μg/mL for (3) and 15-90 μg/mL for (4). The intermediate precisions were below 0.9 % (n=7). The LODs and LOQs were 5 and 10 μg/mL for (1), (2) and (4) and 2 and 6 μg/mL for (3). Average recoveries were between 98.3 and 99.0 % for (1) to (4).

J. Planar Chromatogr. 30, 175-180 (2017). HPTLC of β-amyrin (1) and β-sitosterol (2) in the aerial parts of Tinospora cordifolia and Calotropis gigantia on silica gel with n-hexane – ethyl acetate 3:1. Detection by spraying with p-anisaldehyde reagent. Quantitative determination by absorbance measurement at 530 nm. The hRF values for (1) and (2) were 39 and 26, respectively. Linearity was between 100 and 1400 ng/zone for (1) and (2). LOD and LOQ were 18 and 55 ng/zone for (1) and 30 and 91 ng/zone for (2). The intermediate precision was <2 % (n=6). Recovery rate ranged from 98.4 to 99.3 % for (1) and 98.3 to 99.9 % for (2).

J. Liq. Chromatogr. Relat. Technol. 40, 725-731 (2017). HPTLC of butylated hydroxytoluene (BHT) in Antarctic krill on silica gel with petroleum ether – ethyl acetate – hexane 8:2:1. Detection by spraying with 5 % aqueous ferric chloride followed by heating at 105 °C for 5-10 min. Quantitative determination by absorbance measurement at 600 nm. The hRF value for BHT was 79. Average recovery was 101.8 %.

in different_x000D_ growth stages by thin-layer chromatographic scanning. J. Planar Chromatogr. 31, 190-196 (2018). HPTLC of rupestonic acid (1), vitexicarpin (2), apigenin (3), and luteolin (4) in Artemisia rupestris on silica gel with chloroform – methanol – formic acid – water 650:63:17:7. Quantitative determination by absorbance measurement at 250 nm for (1) and 352 nm for (2) to (4). The hRf values for (1) to (4) were 56, 67, 34 and 18, respectively. Linearity was in the range of 128-725 ng/zone for (1), 70-400 ng/zone for (2), 26-145 ng/zone for (3) and 17-97 ng/zone for (4). The intermediate precision was below 5 % (n=6). Average recoveries for (1) to (4) were 100.0 %, 103.6 %, 98.1 % and 99.9 %.

germplasms from Western Himalayas. Pharmacogn. Mag. 14, 141-146 (2018). HPTLC of precocene I (1) and precocene II (2) in the germoplasms of Ageratum conyzoides on silica gel with toluene – ethyl acetate 49:1. Quantitative determination by absorbance measurement at 300 nm. The hRF values (1) and (2) were 60 and 23, respectively. Linearity was between 100 and 500 ng/zone for both (1) and (2). LOD and LOQ were 5 and 16 μg/mL for both (1) and (2). The intermediate precision was <5 % (n=3). Average recovery was 99.7 % for (1) and 102.9 % for (2).

J. Soil Sci. Plant Nutr. 18, 881-892 (2018). HPTLC of vasicine in Adhatoda zeylanica on silica gel with toluene – methanol – dioxane – ammonia 2:2:5:1. Qualitative identification under UV light at 343 nm. The hRF value for vasicine was 83.