J. Planar Chromatogr. 29, 336-340 (2016). HPTLC of isoalantolactone (1), germacranolide (2), β-sitosterol (3), and lupeol (4) in the roots of Inula cappa on silica gel with toluene ‒ methanol 47:3. Detection by dipping into anisaldehyde reagent followed by heating at 105 ºC until colored bands appeared. Quantitative determination by absorbance measurement at 525 nm. Linearity ranged from 10-70 μg/mL for (1), 10-60 μg/mL for (2), 8-48 μg/mL for (3) and 15-90 μg/mL for (4). The intermediate precisions were below 0.9 % (n=7). The LODs and LOQs were 5 and 10 μg/mL for (1), (2) and (4) and 2 and 6 μg/mL for (3). Average recoveries were between 98.3 and 99.0 % for (1) to (4).

J. Planar Chromatogr. 30, 175-180 (2017). HPTLC of β-amyrin (1) and β-sitosterol (2) in the aerial parts of Tinospora cordifolia and Calotropis gigantia on silica gel with n-hexane – ethyl acetate 3:1. Detection by spraying with p-anisaldehyde reagent. Quantitative determination by absorbance measurement at 530 nm. The hRF values for (1) and (2) were 39 and 26, respectively. Linearity was between 100 and 1400 ng/zone for (1) and (2). LOD and LOQ were 18 and 55 ng/zone for (1) and 30 and 91 ng/zone for (2). The intermediate precision was <2 % (n=6). Recovery rate ranged from 98.4 to 99.3 % for (1) and 98.3 to 99.9 % for (2).

J. Liq. Chromatogr. Relat. Technol. 40, 725-731 (2017). HPTLC of butylated hydroxytoluene (BHT) in Antarctic krill on silica gel with petroleum ether – ethyl acetate – hexane 8:2:1. Detection by spraying with 5 % aqueous ferric chloride followed by heating at 105 °C for 5-10 min. Quantitative determination by absorbance measurement at 600 nm. The hRF value for BHT was 79. Average recovery was 101.8 %.

in different_x000D_ growth stages by thin-layer chromatographic scanning. J. Planar Chromatogr. 31, 190-196 (2018). HPTLC of rupestonic acid (1), vitexicarpin (2), apigenin (3), and luteolin (4) in Artemisia rupestris on silica gel with chloroform – methanol – formic acid – water 650:63:17:7. Quantitative determination by absorbance measurement at 250 nm for (1) and 352 nm for (2) to (4). The hRf values for (1) to (4) were 56, 67, 34 and 18, respectively. Linearity was in the range of 128-725 ng/zone for (1), 70-400 ng/zone for (2), 26-145 ng/zone for (3) and 17-97 ng/zone for (4). The intermediate precision was below 5 % (n=6). Average recoveries for (1) to (4) were 100.0 %, 103.6 %, 98.1 % and 99.9 %.

germplasms from Western Himalayas. Pharmacogn. Mag. 14, 141-146 (2018). HPTLC of precocene I (1) and precocene II (2) in the germoplasms of Ageratum conyzoides on silica gel with toluene – ethyl acetate 49:1. Quantitative determination by absorbance measurement at 300 nm. The hRF values (1) and (2) were 60 and 23, respectively. Linearity was between 100 and 500 ng/zone for both (1) and (2). LOD and LOQ were 5 and 16 μg/mL for both (1) and (2). The intermediate precision was <5 % (n=3). Average recovery was 99.7 % for (1) and 102.9 % for (2).

J. Soil Sci. Plant Nutr. 18, 881-892 (2018). HPTLC of vasicine in Adhatoda zeylanica on silica gel with toluene – methanol – dioxane – ammonia 2:2:5:1. Qualitative identification under UV light at 343 nm. The hRF value for vasicine was 83.

Acta Chromatographica 14, 70-81 (2004) .TLC of thiamine (B1), riboflavin (B2), niacin (B3), pyridoxine (B6), cobalamin (B12) ascorbic acid (C) and folic acid on silica gel and chemical bonded silica gel with 14 mobile phases, three of which giving the best separation: 1) 1-butanol - chloroform - acetic acid - ammonia - water 7:4:5:1:1, 2) benzene - methanol - acetone - acetic acid 14:4:1:1, 3) chloroform - ethanol - acetone - ammonia 2:2:1:1. Detection in visible light, under UV light at 254 nm and 366 nm. Evaluation of other detection methods such as spraying with 0.5 % ether solution of iodine - Dragendorff reagent for B1, 1 % methanol solution of 1-chloro-2,4-dinitrobenzene followed by 3 M NaOH for B3 and B6, 0.4 % methanol solution of 2,6-dichloroquinone-4-chloroimide for B6, 1:1 mixture of 2 % H2SO4 - EtOH and 0.2 % ethanol solution of p-dimethylaminocinnaldehyde for biotin. Quantification by videodensitometry. Discussion of identification of vitamins in biological samples by using different visualization method in combination with Rf values.

J. Chinese Trad. Patent Med. (Zhongchengyao) 25 (7), 543-546 (2004). TLC on silica gel with 1) ethyl acetate - methanol - formic acid - water 10:1:1:1; 2) petroleum ether - chloroform - ethyl acetate - conc. ammonia 80:15:5:1; 3) chloroform - ethyl acetate - methanol - formic acid 35:10:5:1; 4) chloroform - ethyl acetate - methanol - formic acid 70:8:20:3; 5) chloroform - methanol - water 13:6:2. Detection 1) by spraying with 10 % H2SO4 in ethanol and heating at 105 ºC; 2) under UV 254 nm; 3) by spraying with 5 % vanillin - H2SO4 solution and heating at 105 ºC. Identification by finger print technique. Quantification of astragaloside by densitometry at 530 nm.