J. Ethnopharmacol. 168, 50-60 (2015). HPTLC of lupeol (1) and β-sitosterol (2) in the leaves of Glycosmis pentaphylla on silica gel with methanol - toluene 1:9. Quantitative determination by absorbance measurement at 254 nm. The hRF values for (1) and (2) were 66 and 50, respectively.

Chinese J. of Ethnomed. & Ethnopharm. (23), 14-16 (2013). Jiawei Pikang Keli granule is a herbal TCM for the treatment of Pityriasis rosea and eczema. For quality control, TLC on silica gel (1) for Morus alba L., with petroleum ether (60-90 °C) – ethyl acetate 3:2, detection under UV 366 nm; (2) for Cortex Dictamni, with cyclohexane – acetone – ammonia 20:20:1, detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 °C until the zones are clearly visible in daylight; (3) for Angelica sinensis (Oliv.) Diels, with cyclohexane – dichloromethane – ethyl acetate – formic acid 40:10:10:1, detection under UV 366 nm.

J. of China Pharm. 23 (2), 48-51 (2014). Qingbu Tongluo Wan pill is a herbal TCM preparation for the treatment of acute and chronic brucellosis. For quality control, TLC on silica gel (1) for Fructus aurantii, with chloroform – formic acid – water 13:6:2, detection by spraying with 3 % aluminium chloride in ethanol and heating at 105 ºC, detection under UV 366 nm; (2) for Salvia miltiorrhiza Bge., with petroleum ether (60-90 ºC) – ethyl acetate 4:1, detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 ºC, evaluation in daylight; (3) for Corydalis yanhusuo W. T. Wang ex Z. Y. Su et C. Y. Wu, with toluene – acetone 9:2, detection by exposing to iodine vapors for 3 min, evaluation under UV 365 nm; (4) for Radix paeoniae rubra, with chloroform – ethyl acetate – methanol – formic acid 200:25:50:1, detection by spraying with 5 % vanillin in sulfuric acid – ethanol 1:4 and heating mildly until the zonesare visible in daylight. Quantification of salvianolic acid B by HPLC.

Ander in rats. J. Ethnopharmacol. 194, 947-953 (2016). HPTLC of alkaloid (1) and (2) in the whole plants of Hygrophila spinosa on silica gel with hexane – dichloromethane – methanol 5:90:8. Detection by spraying with Dragendorff's reagent and drying at room temperature. Qualitative identification at 520 nm. The hRF values for (1) and (2) were 48 and 56, respectively.

J. Liq. Chromatogr. Relat. Technol. 40, 297-303 (2017). HPTLC of amygdalin on RP-18 with acetonitrile – water 1:1. Quantitative determination by absorbance measurement at 210 nm. The hRF value for amygdalin was 60. Linearity was between 2.5 and 50 μg/zone. LOD and LOQ were 1 and 4 μg/zone, respectively. The intermediate precision was <2 % (n=3). Recovery rate ranged from 99.9 to 100.6 %.

J. Planar Chromatogr. 30, 495-501 (2017). HPTLC of stigmasterol in Lasiurus scindicus on silica gel with toluene ‒ methanol ‒ formic acid 90:10:3. Detection by spraying with anisaldehyde ‒ sulfuric acid reagent, followed by heating at 150 °C for 5 min. Quantitative determination by absorbance measurement at 530 nm. The hRF value for stigmasterol was 27. Linearity was between 2 and 12 ng/zone. LOD and LOQ were 0.07 and 0.2 ng/zone. Average recovery was 99.3 %.

J. Sep. Sci. 41, 518-524 (2018). HPTLC of withanolide S in the leaves of Withania somnifera on silica gel with dichloromethane – toluene – methanol – acetone 15:2:1:1. Detection by spraying with sulfuric acid reagent (180 mL methanol with 20 mL sulfuric acid) followed by heating at 120 °C for 10 min. Quantitative determination by fluorescence measurement at 365/>400 nm. The hRF value for withanolide S was 7. Linearity was between 15 and 500 μg/mL. LOD and LOQ were 30 and 92 μg/mL. The intermediate/interday/intra-day precisions were below 2 % (n=3). Recovery was in the range of 94.6 and 99.1 %.

Braz. J. Pharmacog. 28, 631-639 (2018). HPTLC of 3-O-α-L-rhamnopyranosyl-(1→ 2)-α-L-arabinopyranosyl pomolic acid, 2,4,6-trihydroxy-4-methoxybenzophenone-2-O-β-d-glucoside, hyperoside, geniposidic acid, nicotiflorin, narcissin, randiasaponin IV and rutin in the leaves, stems and roots of Fadogia agrestis on silica gel with chloroform – ethyl acetate – methanol – formic acid 15:30:10:4. Detection by immersion in the anisaldehyde–sulfuric acid reagent, followed by heating at 100 ºC for 3 min. The HPTLC fingerprinting method was suitable for rapid decisive authentication and comparison of differences among samples of identical source.