Chinese J. Mod. Drug Appl. 7 (1), 118-119 (2013). Zhiheng tablets are a herbal TCM for treatment of hyperlipidemia. For quality control, TLC on silica gel 1) for Radix Notoginseng, with dichloromethane – methanol – water 13:7:2, detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 °C, viewing in daylight; 2) for Salvia miltiorrhiza and the standard salvianolic acid A sodium, with dichloromethane – acetone – formic acid 25:10:4, detection by exposing to ammonia vapors for 10 min and viewing under UV 366 nm; 3) for Pericarpium Citri Reticulatae and the standard hesperidin, with ethyl acetate – methanol – water 100:17:13 to a distance of 3 cm and then with toluene – ethyl acetate – formic acid – water 20:10:1:1 to a distance of 8 cm, detection by spraying with 3 % aluminium chloride in ethanol and viewing under UV 366 nm; 4) for Fructus Gardeniae and the standard gardenoside, with ethyl acetate – acetone – formic acid – water 5:5:1:1, detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 °C, viewing in daylight.

J. of Qilu Med. & Pharm. 31 (4), 574-576 (2012). Fufang Jugeng Mahuangjian Tangjiang syrup ll is a herbal TCM preparation for treating cough caused by asthmatic bronchitis. For quality control and to guarantee the safty and effectiveness of the medicine, TLC on silica gel 1) for ephedrine hydrochloride, with n-butanol – formic acid – water 4:1:5, detection by spraying with 0.2 % ninhydrin in acetone and heating at 105 °C, viewing in daylight; 2) for Platycodon grandiflorus, with chloroform – diethyl ether 1:1, detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 °C, viewing in daylight.

J. Planar Chromatogr. 26, 274-278 (2013). HPTLC of oroxylin A in Oroxylum indicum herbal formulations on silica gel with toluene - ethyl acetate - acetic acid 14:5:1. Quantitative determination by absorbance measurement at 275 nm. The hRf of oroxylin A was 80. Linearity was in the range of 250-1500 ng/zone. LOQ was 120 ng/zone. Recovery was in the range of 94.5-101.2 %. Intermediate/interday/intra-day precision was below 2 % (n=5). Comparable results were obatined with a validated HPLC method.

J. Strait Pharm. 24 (7), 75-76 (2012). Shenghua Heji compound oral liquid is a herbal TCM preparation for treatment of postpartum blood circulation, promote uterine contraction, removing blood stasis. For quality control, TLC 1) for Angelica sinensis, on silica gel with benzene – ethyl acetate – glacial acetic acid 2:1:1, detection under UV 254 nm; 2) for Rhizoma chuanxiong, Semen persicae, Glycyrrhizae and the standard ferulic acid, on silica gel with benzene – glacial acetic acid – methanol 30:2:3, detection under UV 366 nm and after spraying with 1 % ferric chloride – 1 % potassium ferricyanide 1:1 in daylight; 3) for Rhizoma chuanxiong and the standard ferulic acid, on neutral alumina layer with petroleum ether (60-90 °C) – chloroform 1:1, detection by spraying with 5 % potassium iodobismuthate in water – hydrochloric acid 200:1 and viewing in daylight.

Chinese J. of Med. Guide 10 (17), 98-99 (2012). Banlian Chongji electuary is herbal TCM preparation for the treatment of virus flu and upper respiratory infections. For quality control the indirubin and indigo contents are determined by TLC on silica gel with petroleum ether (60-90 °C) – ethyl acetate – chloroform 1:1:8. Detection in daylight, quantification by densitometry at 290 nm. The linearity was in the range of 0.1-0.5 µg/zone (n=5, r=0.9999) both for indirubin and indigo. The precision (%RSD, n=5) was 1.2 % for indirubin and 2.2 % for indigo. Recovery by standard addition was 95.5 % (%RSD=4.5 %, n=5) for indirubin, and 96.0 % (RSD%=2.9 %, n=5) for indigo.

Chinese J. of Med. Guide 10 (20), 99-102 (2013). Fufang Xiaoji Zhike Milian Gao ointment is herbal TCM preparation for clearing the body heat caused by inflammation, resolving phlegm and relieving asthma. For quality control, TLC on silica gel 1) for Pericarpium Citri Reticulatae and the standard hesperidin, first over 3 cm with ethyl acetate – methanol – water 99:17:13 and thenover 8 cm with toluene – ethyl acetate – formic acid – water 95:45:5:3, detection by spraying with 2 % aluminium chloride in ethanol and viewing under UV 366 nm; 2) for Schisandra Chinensis and the standard schizandrin A, with the upper phase of petroleum ether (30-60 °C) – ethyl formate – formic acid 15:5:1, detection under UV 254 nm; 3) for Crataegus pinnatifida and the standard ursolic acid, with toluene – ethyl acetate – formic acid – water 39:8:2:1, detection by spraying with 10 % sulfuric acid in ethanol and heating at 80 °C, detection under UV 366 nm. Quantification of tenuifolin by HPLC.

J. Planar Chromatogr. 27, 157-161 (2014). HPTLC of withanoside V (1), withaferine A (2), 1,2-deoxywithastramonolide (3), withanone (4), withanolide A (5), and withanolide B (6) on silica gel with dichloromethane - toluene - methanol - acetone - diethyl ether 13:14:8:3:1. Quantitative determination by absorbance measurement at 235 nm. The hRF values for (1) to (6) were 7, 55, 61, 63, 66 and 77, respectively. Precision was below 2 % (n=3). The LOD and LOQ were 60 and 250 ng/zone for (1), 120 and 350 ng/zone for (2), 80 and 300 ng/zone for (3), (4) and (6), and 60 and 250 ng/zone for (5), respectively. Average recoveries for (1) to (6) were 97, 98, 99, 98, 98 and 99 %, respectively.

Chinese J. of Med. Guide 11 (16), 507-509 (2013). Xiaoxumingtang keli granule is a herbal TCM effective for promoting blood circulation and removing blood stasis, prescribed as an assistant drug for the treatment of stroke, hemiplegia，rheumatis, etc. For quality control, TLC 1) for Saposhnikovia divaricata (Trucz.) Schischk. and the standards isoacteoside and 4’-o-beta-glucopyranosyl-5-o-methylvisamminol on silica gel with the lower phase of chloroform – ethyl acetate – methanol – water 15:40:22:10, detection under UV 254 nm; 2) for Panax ginseng C. A. Mey. and the standards ginsenoside Rg1, Re, and Rb1 on silica gel with chloroform – methanol 4:1, detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 °C until the zones are visible in daylight and under UV 366 nm; 3) for Ligusticum chuanxiong Hort. and the standards ligustrazine and ferulic acid on silica gel with n-hexane – ethyl acetate 9:1, detection under UV 366 nm; 4) for Paeonia veitchii and the standard paeoniflorin, on silica gel with chloroform – ethyl acetate – methanol – formic acid 200:25:50:1, detection by spraying with 5 % vanillin in sulfuric acid – ethanol 1:4 and heating at 105 °C until the zones are visible in daylight; 5) for Ephedra sinica Stapf and the standard (1R,2S)-(-)-ephedrine hydrochloride, on silica gel with chloroform – methanol – concentrated ammonia 40:10:1, detection by spraying with 0.1 % ninhydrin in acetone, heating at 105 °C until the zones are visible in daylight; 6) for Scutellaria baicalensis Georgi and standard baicalin, on plates made of silica gel - sodium acetate – 0.3 % CMC 50:8:150, with ethyl acetate – butanone – formic acid – water 5:3:1:1, detection by spraying with 2 % ferric chloride in ethanol, evaluation in daylight.