Chinese J. of Lishizhen Trad. Med. & Pharm. 23 (2), 401-402 (2012). The dried root of Anemarrhena asphodeloides Bge. is a traditional Chinese medicinal crude drug specially effective for cleaning heat, purging the pathogenic fire, reinforcing body fluid and nourishing the blood, promoting the secretion of saliva or body fluids. Timosaponin B-II is a water soluble saponin extracted from Anemarrhenae. TLC of rhizoma anemarrhenae total steroidal saponins on silica gel with n-butanol – glacial acetic acid – water 4:1:5, detection by spraying with 10 % vanillin in ethanol and heating at 105 °C until the zones are visible, evaluation in daylight.

f.) Wall. ex Nees. (Chinese). Chinese J. of Lishizhen Trad. Med. & Pharm. 22 (6), 1411-1412 (2012). The extracts of Andrographis paniculata, a traditional Chinese medicinal herb, have been widely used as the core ingredient in a variety of traditional Chinese patent medicines, such as Chuanxinlian pills, Chuanxinlian capsules, Lianzhi pills, and some cosmetics and veterinary drugs. The quality of the extracts became significant for guaranteeing the validity, security, stability and homogeneity of the medicines. TLC of the herb extracts on silica gel with chloroform – ethyl acetate – methanol 20:15:2. Detection under UV 254 nm and by spraying with 2 % 3,5-dinitrobenzoic acid in ethanol – 2 mol/L KOH solution 1:1 followed by evaluation in daylight.

Chinese J. of Lishizhen Trad. Med. & Pharm. 22 (8), 2001-2002 (2011). Monkshood and Radix Aconiti agrestis, as traditional Chinese herbal drugs, are usually processed into small pieces ready for decoction and are clinically effective for relieving rheumatic pains. However, in the market some processed drugs were found to be mixed with counterfeits. Presentation of an assay method to investigate the situation of adulteration of these drugs. TLC of the extracts of the drugs 1) for alkaloids, on silica gel with n-hexane – ethyl acetate – methanol 32:18:5 with chamber saturation with ammonia vapors for 20 min, detection by spraying with 5 % potassium iodobismuthate solution. Identification a) by fingerprint comparison with the individual standard component drug; b) by microscopy distinguishing the cluster crystal of calcium oxalate consisted in the thin-wall cells of the drug; 2) for peoniflorin, on silica gel with chloroform – ethyl acetate – methanol – formic acid 40:5:10:1, detection by spraying with 5 % vanillin in sulfuric acid – ethanol 1:4 and heating at 105 °C.

Chinese J. of Northern Pharm. 10 (2), 2-3 (2013). Xiaoer Danzhen granules are a herbal TCM for treating children with focal accumulation of heat, aphthae, hot urination, chicken pox, influenza fever, etc. For quality control, TLC on silica gel 1) for Radix Scutellariae and the standard astragaloside A, with n-butanone – glacial acetic acid – water 7:1:2, detection by spraying with 2 % ferric chloride in 2N HCl – water 1:100, and heating at 105 °C, viewing in daylight; 2) for Fructus Gardeniae and the standard gardenoside, with the lower phase of chloroform – methanol – water 13:7:2, detection by spraying with 10 % sulfuric acid in ethanol, and heating at 105 °C, viewing in daylight; 3) for Radix Angelicae Sinensis, with n-hexane – ethyl acetate – formic acid 45:5:1, detection under UV 366 nm.

J. Strait Pharm. 24 (11), 16-18 (2012). Yangyin Qingfei compound oral liquid is a herbal TCM preparation for treating cough, sore throat, tonsillitis, etc. For quality control, TLC on silica gel 1) for Rehmannia, with chloroform – methanol – water 14:6:1, detection by immersion into vanillin – sulfuric acid – acetic acid 1:10:1000 and heating at 105 °C, viewing in daylight; 2) for Radix Ophiopogonis, with toluene – methanol – glacial acetic acid 800:50:1, detection under UV 254 nm. Quantification of catalpol by HPLC.

J. of Inform. on Trad. Chinese Med. 30 (3), 61-63 (2013). Naodesheng Koufuye oral liquid is a herbal TCM preparation for treatment of cerebral arteriosclerosis and ischemic stroke. For quality control, TLC on silica gel 1) for Flos Carthami, with cyclohexane – ethyl acetate 11:6, detection by exposure to ammonia vapors and viewing under UV 366 nm; 2) for Radix Puerariae and the standard puerarin, with chloroform – ethyl acetate – methanol – water 15:40:22:10, detection under UV 366 nm; 3) for Rhizoma Ligustici Chuanxiong and the standard ferulic acid, with benzene – chloroform – methanol 2:2:1, detection under UV 254 nm. Quantification of ginsenoside Rb, ginsenoside Rg and notoginsenoside R by HPLC.

J. of Modern Trad. Chinese Med. 15 (4), 320-323 (2013). Leifengshi Jiaonang capsules are a herbal TCM preparation for the treatment of rheumatism, ankylosing spondylitis, and lupus erythematosus. For quality control, TLC on silica gel 1) for Astragalus membranaceus (Fisch.) Bunge and the standard astragaloside A, with chloroform – methanol – water 13:7:2, detection by spraying with 10 % sulfuric acid in ethanol, heating at 105 °C and viewing in daylight and under UV 366 nm; 2) for Angelica sinensis, with petroleum ether (60-90 °C ) – diethyl ether – acetic acid 20:20:1, detection under UV 366 nm 3) for Epimedium sagittatum, with ethyl acetate – formic acid – butanone – water 10:1:1:1, detection under UV 366 nm. Quantification of ferulic acid by HPLC.

Jiangxi J. of Trad. Chinese Med. 44 (367), 58-60 (2013). Zhongfeng Fuyuan Koufuye oral liquid is a herbal TCM preparation for the treatment of post stroke shoulder hand syndrome during stroke recovery and sequela period, and constipation after stroke. For quality control, TLC on silica gel 1) for Astragalus membranaceus (Fisch.) Bunge., with n-butanol – ethyl acetate – 10 % diluted ammonia 4:1:5, detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 °C and viewing in daylight; 2) for Pericarpium Citri Reticulatae, with toluene – ethyl acetate 3:2, detection by heating at 105 °C for 3 min and viewing under UV 366 nm; 3) for Paeonia veitchii, with toluene – ethyl acetate – isopropanol – methanol – ammonia 20:10:5:5:1, detection by exposure to ammonia vapors for 15 min and spraying with 5 % vanillin in sulfuric acid – ethanol 1:4 and heating at 105 °C, viewing in daylight. Quantification of astragaloside A by HPLC.