Chinese J. of Jiangxi Ind. (3), 68-70 (2012). Genianan capsules are a herbal TCM for treating some diseases during women’s menopause such as tinnitus, palpitation, insomnia, blood pressure instability, etc. For quality control, TLC on silica gel 1) Radix Polygoni Multiflori and Caulis Polygoni Multiflori and standard emodin, with toluene – ethyl acetate – formic acid 15:2:1, detection under UV 366 nm; 2) for Fructus Schisandrae Chinensis and the standards deoxyschizandrin and schizandrin B, with petroleum ether (30-60 °C) – ethyl acetate – formic acid 15:5:1, detection under UV 254 nm; 3) for Radix Ophiopogonis, with chloroform – acetone 22:5, detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 °C, viewing in daylight; 4) Rhizoma Curculigins and the standard curculigoside, with ethyl acetate – methanol – formic acid 100:10:1, detection by spraying with 0.1 mol potassium ferricyanide – 0.1 mol ferric chloride 1:1 and viewing in daylight.

Chinese J. Ethnopharm.(9), 36-38 (2012). Luman-12 is a traditional Mongolian herbal medicine preparation for treatment of cough, asthma, etc. For quality control of the main component herbal drugs TLC on silica gel 1) for Radix Polygalae, with toluene – ethyl acetate – formic acid 28:8:1, detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 °C, viewing in daylight; 2) for Radix Glycyrrhizae, with toluene – ethyl acetate – formic acid 10:8:1, detection under UV 366 nm; 3) for Piper nigrum, with benzene – ethyl acetate – acetone 7:2:1, detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 °C, viewing in daylight.

Chinese J. of Jilin Med. Col l. 33 (1), 8-10 (2012). Citrus limon is a class of traditional Chinese medicinal plants used in various TCM preparations. To guarantee the safety and effectiveness it is significant to identify the authenticity of the crude drugs. TLC of 11 varieties of standard crude drug 1) for alkaloids with the standard synephrine, on 0.5 % NaOH containing silica gel with chloroform – ethanol – ammonia 5:5:1, detection by spraying with 1 % ninhydrin in ethanol and heating at 105 °C, viewing under UV 366 nm; 2) for flavonoids with the standard hesperidin and naringin, on silica gel with ethyl acetate – methanol – water 100:17:13, detection by spraying with 1 % aluminiumchloride in ethanol and viewing under UV 366 nm. The method demonstrated the merits of TLC, such as high throughput, fast analysis, no interferences, specificity, intuitive use and can be a base of establishing the operational standard method for the quality control of the crude drugs.

Chinese J. of Xizang Sci. & Technol. 231, 48-49 (2012). Shanhu Qishiwan pills are a traditional Tibetan herbal medicine used for relieving uneasiness of mind and body, invigoration of blood circulation and relief of pain. For quality control, TLC on silica gel with n-hexane – ethyl acetate 4:1, detection under UV 366 nm.

J. Planar Chromatogr. 26, 331-335 (2013). HPTLC of betulinic acid in the bark and leaves of Dillenia indica, Dillenia pentagyna, Ziziphus jujube and Ziziphus mauritiana on silica gel with toluene - chloroform - ethanol 4:4:1. Detection by dipping into anisaldehyde - sulfuric acid reagent, followed by heating at 80 ºC for 15 min. Quantification by absorbance measurement at 525 nm. The hRf of betulinic acid was 71. Linearity was in the range of 100-1200 ng/zone. LOD and LOQ were 4 and 11 ng/zone, respectively. Recovery was in the range of 99.5-101.7 %. Intermediate/interday/intra-day precision was below 2 %.

J. of Chinese Med. 26 (169), 716-718 (2012). Shengma Gegen granules are a herbal TCM preparation for the treatment of viral hepatitis type C, drug-induced liver diseases, and postherpetic neuralgia. For quality control, TLC on silica gel 1) for Rhizoma Cimicifugae, with benzene – chloroform – glacial acetic acid 12:2:1, detection under UV 366 nm; 2) for Radix Paeoniae Rubra, with chloroform – ethyl acetate – methanol – formic acid 200:25:50:1, detection by spraying with 2 % vanillin in sulfuric acid – ethanol 1:200 and heating mildly until the zones are visible in daylight. Quantification of puerarin by HPLC.

Chinese J. of Med. Guide 11 (16), 509-510 (2013). Zhixie Baotong Keli granules are a herbal TCM preparation for the treatment of dysentery, abdominal pain, dry mouth, nausea, and vomiting. For quality control, TLC on silica gel 1) for Tetradium ruticarpum, with cyclohexane – ethyl acetate – methanol – triethylamine 15:5:1:1, detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 °C for 4 min and viewing under UV 366 nm; 2) for Pogostemon cablin, with petroleum ether (60-90 °C) – ethyl acetate 19:2, detection by spraying with 1 % vanillin in sulfuric acid – ethanol 1:4 and heating at 105 °C until the zones are visible in daylight; 3) for Fructus Amomi and the standard bornyl acetate, with cyclohexane – ethyl acetate 22:1, detection by spraying with 5 % vanillin in sulfuric acid – ethanol 1:4 and heating at 105 °C until the zones are visible in daylight.

J. of China Pharm. 27 (7), 725-728 (2013). Fufang Hafangji Keli granules, with Aristolochia fangchi as key component drug, are a herbal TCM preparation for improving the liver function, and are used for treatment of acute and chronic hepatitis, cirrhosis, and fatty liver. TLC of the extracts and standards (+)-tetrandrine and fangchinoline on silica gel with cyclo hexane – chloroform – acetone – methanol 10:6:1:1, detection by spraying with (bismuth subnitrate – glacial acetic acid – water 1:10:40) – potassium iodide – glacial acetic acid – water 1:1:4:20. Quantification of (+)-tetrandrine and fangchinoline by HPLC.