60th Indian Pharmaceutical Congress PG-323 (2008). HPTLC of glycyrrhizin, apigenin and kaempferol in methanolic extracts and glycyrrhetinic acid and quercetin in hydrolized extracts of Glycyrrhiza glabra on silica gel with ethyl acetate - methanol - acetic acid - water 40:5:5:10 (for glycyrrhizin), toluene - ethyl acetate - methanol - formic acid 30:15:1:2 (for kaempferol and quercetin), ethyl actate - ethanol - water - ammonia 65:20:4:1 (for glycyrrhetinic acid). Quantitative determination by absorbance measurement at 254 nm (glycyrrhetinic acid and apigenin), 258 nm (glycyrrhizin) and 280 nm (kaempferol and quercetin). The plant was found to contain 1.07 % glycyrrhizin, 0.64 % glycyrrhetinic acid, 0.007 % apigenin, 0.03 % kaempferol and 0.24 % quercetin.

Abstract No. F-365, 61st IPC (2009). HPTLC of rivastigmine hydrogen tartrate on silica gel with methanol - 25 % ammonia - acetic acid 200:7:2. The hRf value was 54. Quantitative determination by absorbance measurement at 215 nm. The method was linear in the range of 1-10 µg/band.

J. Planar Chromatogr. 23, 173-179 (2010). Many manuscripts and already published articles on analytical procedures to be used in pharmaceutical quality control are characterized by several typical methodological failures and misconceptions. The autors present a collection of typical failures, misconceptions, and misleading data from articles published over the last two years in seven well-known chromatographic publications and provide at the same time a list of references describing optimum approaches to validation of specific TLC/HPTLC procedures. In particular, method specificity, linearity, accuracy, and precision very often are not determined properly and in accordance with best practise.

J. Braz. Chem. Soc. 21, 441-446 (2010). HPTLC of ochratoxin A in wine on silica gel with toluene - ethyl acetate - chloroform - formic acid 6:3:1. Quantitative determination by absorbance measurement at 366 nm, using a CCD camera followed by images processing using the software ImageJ. Linearity was between 0.8 and 32 µg/L. The intra-day and inter-day precisions had a RSD lower than 9.9 % and 11.5 %, respectively. LOD was 16 ng/zone while LOQ was 100 ng/zone. The proposed method is a simple, efficient and low cost tool for quantitative analysis of ochratoxin A in wine samples.

IPA Convention, 2010, RA-PO 03. HPTLC of edaravone in samples of human plasma (purified by liquid-liquid extraction) on RP-18 with n-butanol - methanol - diethyl ether 1:8:1. The compound was well resolved with an hRf value of 81. Densitometric evaluation at 240 nm. The limit of detection and quantification was 25 ng and 150 ng respectively. The linearity was 600-2400 ng/band. Recovery (%) based on analysis of spiked sample was more than 65 %.

Abstract No. C-161, 61st IPC (2009). An HPTLC method is reported for estimation of andrographolides bitter principles in Andrographis paniculata, popularly known as kalmegh. HPTLC of methanolic and water extracts on silica gel with chloroform - methanol 7:1 in a saturated twin trough chamber. Quantitative evaluation by absorbance measurement at 231 nm. The method was found to be linear in the range of 1-5 µg/band. Both extracts were found to contain andrographolides. Maximum yields of andrographolides were observed in extracts prepared by refluxing.

International Seminar on Herbal Drug Research, PN-017 (2009). HPTLC of thiocolchicoside and diclofenac sodium on silica gel with toluene - acetone - methanol - formic acid 500:200:200:1. Quantitative determination by absorbance measurement at 280 nm. The method was linear in the range of 160-800 ng/band (thiocolchicoside) and 1000-5000 ng/band (diclofenac sodium). The recovery was in the range of 99.2-100.9 for both compounds.

Encyclopedia of Chromatography Third Edition 1, 950-953 (2009). The author describes the standard guides of the ASTM designations for forensic writing ink comparison and identification using TLC. Recent advances such as software for visual comparison of chromatograms are included. In addition, new applications of TLC in the field of forensic ink analysis and comparison with spectrometric and chromatographic methods are also described.