J. Planar Chromatogr. 25, 30-35 (2012). HPTLC of rutin (1), narcissin (2), nicotiflorin (3) and isoquercitrin (4) on silica gel with ethyl acetate - 1,2-dichloroethane - acetic acid - 85 % formic acid - water 100:25:10:10:8. Quantitative determination by absorbance measurement at 360 and 387 nm. Intraday and interday precisions were in the range of 1.5-1.9 % and 1.6-1.9 %, respectively. Recovery was found to be 98.2-101.4 % for (1), 98.0-101.1 % for (2), 98.2-100.5 % for (3) and 98.1-101.9 % for (4).

J. Planar Chromatogr. 25, 523-527 (2012). TLC of a mixture of 12 non-steroidal anti-inflammatory drugs on silica gel with ethyl acetate - toluene - methanol - acetic acid 40:40:1:1. Quantitative determination by absorbance measurement at 254 nm. Limits of detection were in the range of 5-500 ng/zone. The method showed comparable results to a validated HPLC method.

CBS 109, 2-4 (2012). Extraction of arabica coffee with 2 to 50 % robusta coffee by accelerated solvent extraction with tBME. A part was saponified with10 % ethanolic KOH solution for 2 h. HPTLC of coffee extracts and standards 16-O-methylcafestol (16-OMC) and 16-OMC esters on silica gel with toluene - ethyl acetate - acetic acid 93:7:1 for 16-OMC esters and with tBME - chloroform 1:1 for 16-OMC. Detection by spraying with vanillin sulfuric acid (1 g in 250 mL ethanol and 2 mL of sulfuric acid, prepared freshly) and heating for 1 min at 80 °C. Quantitative absorption measurement at 530 nm. The LOD was 163 ng/band. After saponification the LOD of free 16-OMC was 43 ng/band. Thus 2 % robusta can be detected in a coffee blend.

J. Planar Chromatogr. 25, 150-155 (2012). HPTLC of methocarbamol (1) and its related substance guaifenesin (2) in two ternary mixtures with ibuprofen (3) and diclofenac potassium (4) on silica gel with ethyl acetate - acetone - triethylamine 62:35:6 + 1 drop formic acid. Quantitative determination by absorbance measurement at 222 nm for the first mixture and 278 nm for the second mixture. The hRf values for agents (1) to (4) were 78, 54, 14 and 12, respectively. Linearity was in the range of 2-12 µg/band for (1), 2-10 µg/band for (2), 4-20 µg/band for (3) and 0.2-2.2 µg/band for (4). The intermediate/inter-day/intra-day precision was below 1.5 %. Mean recovery for (1) to (4) was between 100.7 and 100.8 %.

Phytochem. Anal. 24, 230-247 (2013). HPTLC of lupeol in the leaves of Ficus racemosa on silica gel with toluene - chloroform - ethyl acetate 10:2:1 + 1 drop glacial acetic acid. Quantitative determination by absorbance measurement at 366 nm. The hRf of lupeol was 31. Average recovery was in the range of 98.8-99.4 %. The intra- and inter-assay precision was below 0.2 %.

J. Planar Chromatogr. 26, 37-42 (2013). HPTLC of hydrocortisone acetate (1) and 2-phenoxyethanol (2) in cream on RP-18 with water-methanol 4:11. Quantitative determination by absorbance measurement at 270 nm. Linearity was in the range of 4.2-13.0 µg/zone for (1) and 0.8-4.3 µg/zone for (2). LOD and LOQ were 0.6 and 1.7 µg/zone for (1) and 0.2 and 0.5 µg/zone for (2), respectively. Intermediate precision was below 2 %.

J. Planar Chromatogr. 26, 267-273 (2013). HPTLC of glabridin in a Glycyrrhiza glabra formulation on silica gel with toluene - dichloromethane - ethyl acetate 1:1:1. Quantitative determination by absorbance measurement at 287 nm. The hRf value for glabridin was 57. Linearity was in the range of 25-500 ng/zone. LOD and LOQ were 10 and 25 ng/zone. Recovery was in the range of 97.3-103.2 %. Intermediate/interday/intra-day precision was below 2 % (n=6).

J. Planar Chromatogr. 26, 375-378 (2013). TLC of estrone, estradiol, and estriol on silica gel with chloroform - ethyl acetate - acetone 6:2:1. Detecion by dipping into (1) 10 % solution of phosphomolybdic acid (PMA) in methanol, followed by heating at 100 ºC for 10 min; (2) 0.2 % ceric ammonium sulfate in phosphoric acid, followed by heating at 110 ºC for 10 min; (3) 0.2 g manganese(II) chloride in 30 mL water, 30 mL methanol and 2 mL concentrated sulfuric acid, followed by heating at 100-120 ºC for 10-15 min; and (4) 1 g of vanillin in 25 mL of ethanol, 25 mL of distilled water, and 35 mL of ortho-phosphoric acid (85 %), followed by heating at 120-160 ºC for 5-15 min. The lowest detection limit for estrone (75 ng/zone) was achieved using (1) and (3), whereas for estradiol and estriol (both 4.7 ng per zone) by using (2).