Preparative Biochemistry and Biotechnology 40, 337-346 (2010). TLC of dexrabeprazole (DEX) and domperidone (DOM) in combined dosage form on silica gel with acetone - toluene - methanol 9:9:1. Quantitative evaluation by absorbance measurement at 285 nm. The hRf of DEX and DOM was 49 and 24, respectively. The linearity range for DEX was 50-350 ng/band (r=9960) and for DOM 100-700 ng/band (r=9982).

J. Liq. Chromatogr. Relat. Technol. 34, 1502-1517 (2011). HPTLC of irinotecan in bulk drug and injectable formulations on silica gel with acetone - ethyl acetate 17:3 + 1 drop acetic acid. Quantitative determination by absorbance measurement at 366 nm. The hRf of irinotecan was 31. Linearity was 50-500 ng/zone. The LOD and LOQ was found to be 10 and 33 ng/zone. The intra-day precision was 4.3 % (n = 6) and inter-day precision over three different days was 3.1 %. Intra-day and inter-day accuracy were 96.9-100.3 % and 96.5-98.7 %, respectively. Recovery (by standard addition) ranged from 94.6-101.4 %.

J. Planar Chromatogr. 24, 507-512 (2011). HPTLC of flavonoids (quercetin and kaempferol) and biflavonoids (sciadopitysin, ginkgetin, and bilobetin) in aqueous methanolic extracts of Ginkgo biloba on RP-18 by double development with 1) acetonitrile - water - methanol - formic acid 20:20:1:0.005 and 2) acetonitrile - water - methanol - formic acid 20:17:1:0.005. Quantitative determination by densitometry in absorption mode at 254 nm. LOD and LOQ were in the range of 0.12-0.37 and 0.60-1.85 µg/zone, respectively. Linearity was found over the concentration range of 0.5-4.0 µg/zone for quercetin, kaempferol, sciadopitysin, ginkgetin, and bilobetin with correlation coefficients r = 0.9990, 0.9922, 0.9939, 0.9974, and 0.9706, respectively. Average recoveries were in the range of 97.7-100.4 %.

J. Ethnopharmacol. 137, 99-104 (2011). HPTLC of karanjin in rabbit plasma on silica gel with dichloromethane - toluene - methanol 35:15:3. Qualitative evaluation under UV 366 nm and quantitative determination by densitometry at 366 nm. Linearity was between 1.0 and 15.0 µg/mL. LOD and LOQ were 0.5 and 1.0 µg/mL, respectively. The inter-day and intra-day accuracies were 97.1 % and 92.2 %, respectively. Recovery (by standard addition) was between 98.2 % and 99.9 %.

Volumes 1-3, CBS Publishers & Distributors, New Delhi, India (2013). The first volume provides a comprehensive introduction to the HPTLC technique, including details for each HPTLC step as well as various factors which influence the performance of a HPTLC analysis. Then presented over 3 volumes, 528 protocols for the HPTLC analysis of pharmaceutical formulations follow. Each protocol provides details on the preparation of samples and standards, chromatographic equipment, parameters for densitometric evaluation, chromatographic conditions, including stationary phase, mobile phase, standard and sample application, chamber saturation, relative humidity, quantity of mobile phase, temperature, migration distance and other critical parameters. References to the original publication are given as well as comments on the validation or any comparative study. Each protocol is illustrated with a typical densitogram, structures of the compounds analysed and overlaid UV spectra of compounds analysed (for selection of suitable wavelength for densitrometric scanning). All in all a comprehensive collection of protocols for pharmaceutical formulations.

J. Liq. Chromatogr. Relat. Technol. 35, 2548-2564 (2012). HPTLC of tocopherol acetate in pharmaceutical preparation on silica gel with chloroform - cyclohexane 11:9. Quantitative determination by absorbance measurement at 272 nm. The hRf values for tocopherol acetate and its related substance were 47 and 38, respectively. Limits of detection and quantification were 50 and 150 ng/zone, respectively. The intermediate/inter-day/intra-day precision was below 1.4 % (n=3). Recovery was between 99.8 and 101.5 %, respectively. A better separation of tocopherol acetate was obtained using NP-TLC technique than by RP-TLC technique.

J. Planar Chromatogr. 25, 290-294 (2012). HPTLC of withaferine A (1), 1,2 deoxy-withastramonolide (2), withanolide A (3), and withanolide B (4) in Withania somnifera on silica gel with dichloromethane - toluene - methanol - acetone - diethyl ether 15:15:6:2:2. Quantitative determination by absorbance measurement at 235 nm. The hRf values of (1) to (4) were found to be 58, 61, 68 and 79, respectively. Linearity was in the range of 200-1200 ng/band. Average recoveries were 98, 99.5, 98 and 99 % for compounds (1) to (4), respectively.

Quim. Nova. 35, 411-415 (2012). HPTLC of nifedipine in human serum on silica gel with chloroform - ethyl acetate - cyclohexane 19:2:2. Quantitative determination by absorbance measurement at 238 nm. The hRf of nifedipine was 31. Linearity was in the range of 0.02-0.25 ng/µL. Limits of detection and quantification were 0.7 and 0.9 ng/band, respectively. Intraday precision was in the range of 0.6-3.6 % (n=3) and inter-day precision was in the range of 1.2-3.6 % (n=9). Recovery was between 93 and 102 %.