60th Indian Pharmaceutical Congress PA-199, (2008). HPTLC of trifluoperazine and hydrochloride and chlordiazepoxide on silica gel with carbon tetrachloride - acetone - triethylamine 12:6:1. Quantitative determination by absorbance measurement at 262 nm. The method was linear in the range of 20-280 ng/spot and 50-700 ng/spot for trifluoperazine HCI and chlordiazepoxide. The method was suitable for routine quality control of combined dosage form.

J. Liq. Chromatogr. Relat. Technol. 31, 1925-1942 (2008). HPTLC of benzo[a]pyrene, benzo[b]fluoroanthene, benzo[k]fluoroanthene, benzo[ghi]perylene, fluoroanthene, 2-methylanthracene, and indeno[1,2,3-cd]pyrene on silica gel impregnated with caffeinesolution (by dipping in a solution of 2 g caffeine in 120 mL acetonitrile for 20 min, followed by drying for 15 min at 120 °C) with isopropyl acetate - acetonitrile 7:3 in a twin trough chamber at -20 °C. For fluorescence enhancement the plates were dipped in a solution of paraffin - n-hexane 1:1 and dried for 1 min in cold air. Quantitative determination by fluorescence measurement at 366/>400 nm. The method can be applied for control of the limit levels of the six polycyclic aromatoc hydrocarbons in water. The limits of quantitation were 0.08-0.44 ng/band depending on the substance. Linearity showed coefficients of correlation = 0.9920. The recoveries by stir bar sorptive extraction (n = 3) were between 87-100 % depending on the substance. The whole procedure was optimized to reach a sample throughput of 30 water samples, inclusive sample preparation by stir-bar sorptive extraction (SBSE), per 8-hour day.

Acta Chromatographica 6, 7-14 (1996). HPTLC of fructose, glucose and sucrose on a channeled silica gel plate with concentration zone with acetonitrile - water 17:3 for three times (freshly prepared each time, taking 15 min per run) with chamber saturation for 10-15 min. Before, just the silica gel layer was impregnated by spraying with 0.10 M sodium bisulfate solution, and subsequently with citrate buffer (1:10 dilution of citrate buffer with water, pH 4.8), each followed by drying. Detection by spraying with 1-naphthol-sulfuric acid reagent, followed by heating at 100-110 °C for 5-10 min. Quantitative determination by absorbance measurement at 515 nm. The hRf values of fructose, glucose and sucrose were 47, 43, and 28, respectively, and selectivity regarding matrix was given. No zones other than the sugars were detected in sample chromatograms because of the selectivity of the detection reagent and the retention of the beverage components in the preadsorbent. Correlation coefficients (r) for linear regression of the calibration curves typically ranged from 0.90-0.99, with an average of 0.97. The precision in matrix was 2.5 % (n = 5). The mean reproducibility of the twofold sample analyses was 4 %, ranged between 0.45 % and 7.5 %. The accuracy of the method was 94.6 %, 97.0 % and 90.8 % for sucrose, glucose and fructose, respectively. Sugar concentrations in the samples ranged from 18.4-34.3 mg/mL.

Anal. Chem. 81, 3858-3866 (2009). HPTLC of microcystin LR and nodularin on silica gel with 1-propanol - ethyl acetate - water 3:5:2 with 5 % acetic acid. Detection and quantification by UV spectroscopy at 232 nm and direct identification of separated analytes on the HPTLC plate by IR-MALDI-o-TOF MS. The detection limit was 3-5 ng/zone. For detection of peptides, plates were cut and sprayed with a solution of p-anisaldehyde, followed by heating at 105 °C until purple-blue peptide spots appeared.

J. Chinese Trad. Patent Med. 30 (12), 1997-1800 (2008). TLC of Hongjinchan granule extracts on silica gel with 1) n-butanol - glacial acetic acid - water 7:1:2; and 2) chloroform - ethyl acetate 7:3. Detection 1) under UV 365 nm; 2) by spraying with 2 % iron(III) chloride in ethanol; 3) by exposure to ammonia vapor for approx. 15 min. Identification by comparison with the standards of the component drugs. Quantification of scutellarin by HPLC.

J. Planar Chromatogr. 22, 207-210 (2009). HPTLC of dexibuprofen on silica gel (prewashed with methanol) in a twin trough chamber with hexane - ethyl acetate - glacial acetic acid 15:5:1. Quantitative determination by absorbance measurement at 217 nm.

J. Planar Chromatogr. 21, 251-258 (2008). TLC of tiaprofenic acid, ketoprofen, naproxen, dexibuprofen, flurbiprofen, alminoprofen, and ibuprofen on silica gel with chloroform - acetone - toluene 12:5:2 and chloroform - ethyl acetate 1:1. Quantitative determination by absorbance measurement at 225 nm (for naproxen, dexibuprofen, and ibuprofen) and at 270 nm (for tiaprofenic acid, ketoprofen, flurbiprofen, and alminoprofen).

Rapid Commun. Mass Spectrom. 23, 2711-2723 (2009). HPTLC in combination with matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) was used for the analysis of complex lipid mixtures. For the separation of lipids one-dimensional HPTLC on silica gel aluminum foil was used with a two-phase mobile phase. The combination with MALDI-MS allowed the identification of 70 distinct lipid species and the analysis of even minor lipid classes from only very small volumes of human plasma (50 µL).