Research J. Pharm. and Tech. 3(2), 559-561 (2010). TLC on silica gel with toluene - acetonitrile - formic acid 50:50:3 with chamber saturation for 30 min. The hRf value was 27 for perindopril and 55 for losartan. Densitometric evaluation at 212 nm. The method was linear in the range of 5-30 µg/band and 1-10 µg/band for losartan and perindopril respectively. The average recovery was 98.2-99.9 %.

J. Planar Chromatogr. 23, 298-303 (2010). TLC of oxaprozin on silica gel with n-hexane - chloroform - glacial acetic acid 4:1:1 with chamber saturation for 15 min at room temperature. The hRf value of oxaprozin was 56. Quantitative determination by absorbance measurement at 286 nm. The limit of detection and quantification was 16 and 40 µg/mL, respectively. Linearity was between 33 and 433 µg/mL. The recovery was between 98.9 and 101.6 % at different spiking levels. Intra-day precision (%RSD) at different concentration levels was 0.86-1.03 % and inter-day precision 0.99-1.15 %. Oxaprozin was subjected to stress tests (acidic and alkaline hydrolysis) and the method was able to separate the degradation products from the main compound.

B. Fried, R. Toledo (Eds): Biomphalaria snails and larval trematodes. Springer 20119. Chapter 4. This book chapter reviews TLC, HPTLC and other methods for the analysis of larval trematodes from Biomphalaria snails. The analysis of different classes of organic compounds is described: neutral and polar lipids, glycolipids, lutein, beta-carotene, sugars and amino acids. Various TLC and HPTLC phases and developing solvents were used.

International Journal of Pharma and Bio Sciences 1(4), 242-245 (2010). HPTLC of andrographolide in Andrographis paniculata and a polyherbal Asava formulation on silica gel with benzene – ethyl acetate 1:1. The hRf value of andrographolide was 10. Quantitative determination at 220 nm. The method was linear in the range of 360-660 ng/band. The andrographolide content of the sample of Andrographis paniculata was 237.2 µg/100 mg, where as Asava contained 41.8 µg/5 mL. The average recovery of andrographolide by standard addition method was 97.7 %.

62nd Indian Pharmaceutical Congress Abstract No. F-234 (2010). HPTLC of atorvastatin and telmisartan on silica gel (pre-washed with methanol and dried at 60 °C for 5 min) with chloroform – benzene – methanol – glacial acetic acid 60:30:10:1. The hRf values were 23 (telmisartan) and 56 (atorvastatin). Quantitative determination by absorbance measurement at 265 nm. The method was linear in the range of 40-200 ng/band for telmisartan and 10-50 ng/band for atorvastatin.

62nd Indian Pharmaceutical Congress Abstract No. F-389 (2010). TLC of amlodipine and telmisartan on silica gel with chloroform – methanol – toluene 10:7:3. The hRf values were 23 and 75 for amlodipine besylate and telmisartan respectively. Quantitative determination by absorbance measurement at 238 nm. The sample was also analysed by spectophotometry and the results by both methods were comparable.

var hirsuta. 62nd Indian Pharmaceutical Congress Abstract No. F-238 (2010). TLC of the two marker compounds bebeerine and oleanolic acid from the roots of Cissampelos pareira Linn. on silica gel with toluene – ethyl acetate – diethylamine 7:2:1 for bebeerine and toluene – ethyl acetate – formic acid 70:30:3 for oleanolic acid. The hRf value was 20 (bebeerine) and 56 (oleanolic acid). Quantitative determination by absorbance measurement at 254 nm and under visible light after spraying with dragendorff’s reagent (bebeerine) or anisaldehyde sulfuric acid reagent (oleanolic acid).

J. Pharm. Biomed. Anal. 54, 422-425 (2011). HPTLC of aloin in several aloe dried extracts and related commercial formulations on silica gel with ethyl formate – methanol – water 200:29:20. Evaluation under 254 nm. Detection by immersion in 10 % H3BO3 in methanol, followed by heating at 110 °C for 10 min. Quantitative determination by fluorescence measurement at 365/K540 nm.