Ind. J. Pharma. Science 71 61, 644-650 (2009). HPTLC of the novel antihyperlipidemic agent LM-13765 in rabbit plasma on silica gel (pre-washed with methanol) with benzene - methanol 4:1 in a saturated chamber at 25 °C. Densitometric evaluation at 314 nm. The method was linear in the range of 12.5-100 ng/band. Also HPTLC of LM-13765-C, a metabolite of the parent compound LM-13765, on silica gel with toluene - methanol 23:2. Densitometric evaluation at 274 nm. Details are provided on the complete extraction procedure for the extraction of the parent compound and its metabolite from plasma.

International Seminar on Herbal Drug Research, PN-011 (2009). HPTLC of harmaline in alcoholic extracts of Peganum harmala on silica gel with chloroform - acetone - diethyl amine 5:4:1 in a saturated chamber at 25 °C. The hRf of harmaline was 50. Quantitative determination by absorbance measurement at 351 nm.

J. Planar Chromatogr. 23, 208-211 (2010). HPTLC of prednisolone (and hydrocortisone as impurity) on silica gel (prewashed with methanol) with chloroform - methanol 19:1 in a twin-trough chamber previously saturated for 30 min. Quantitative determination by scanning densitometry at 250 nm. Linearity was in the range of 2-10 µg/band (r = 0.9967, calculated via peak area). LOD and LOQ was 200 and 600 ng/spot, respectively. Recovery was 100.0 % for prednisolone. Repeatability was 0.74 % and the inter-day (n = 6) and intra-day (n = 12) precision was 2.6 and 2. 7 %, respectively.

J. Planar Chromatogr. 23, 119-122 (2010). HPTLC of artemether and lumefantrine on silica gel with n-hexane - ethyl acetate 4:1 at room temperature in a twin-trough chamber saturated for 15 min. Quantitative determination by densitometric scanning in reflectance mode at 357 nm. The method is linear (r2 > 0.995), precise (RSD < 2 %), accurate (average recovery of 100.5 % for artemether and 99.5 % for lumefantrine), specific, and robust. LOD and LOQ for artemether were 50 and 150 ng/band, respectively, and those for lumefantrine were 300 and 900 ng/band, respectively.

Encyclopedia of Chromatography Third Edition 1, 1640-1647 (2009). This review describes densitometry as the most widely used quantitative TLC method. The principles and theory of densitometry, as well as the instrumental design and data handling are also described. It also mentions in detail the applications and practical aspects of densitometry and finally describes the advantages of TLC/densitometry compared to HPLC.

J Young Pharm 1(4), 359-363 (2010). TLC of efavirenz on silica gel with acetonitrile - water - glacial acetic acid 12:8:1 in a twin-trough chamber saturated for 10 min. Densitometric evaluation at 247 nm. The method was linear in the range of 10-400 ng/band. The average recovery was 99.7 %.

A chromatographic study. J. Planar Chromatogr. 23, 277-281 (2010). TLC of cardiolipin, monolysocardiolipin, and phosphatidylethanolamin plasmalogen on silica gel and alumina pre-washed sequentially with chloroform - methanol 2:1 and acetone. For one-dimensional chromatography the mobile phase was 1-propanol - chloroform - ethyl acetate - methanol - water 50:50:50:21:18. Detection of phospholipids with thionine reagent. Two-dimensional chromatography in the first direction with the mobile phase mentioned before and in the second dimension with hexane - diethyl ether 4:1 after hydrolysis with 1 % hydrochloric acid to reveal alkenyl phospholipids. Detection by spraying with leucofuchsin reagent. TLC of choline lipids, phosphatidylcholine, and sphingomyelin on alumina with chloroform - methanol - water 65:30:4. Detection by treatment with Biebrich scarlet. Densitometric evaluation at 600 nm for thionine reagent and at 560 nm for leucofuchsin reagent.

Rasayan J. Chem. 2(3), 720-723 (2009). HPTLC of cefetamet on silica gel with toluene - chloroform - methanol 3:6:1. The hRf value of cefetamet was 35. Densitometric evaluation at 236 nm. The method was linear in the range of 1-5 µg/band. The recovery was 99.3-101.5 %. The method was found suitable for routine quality control.