J. Planar Chromatogr. 27, 140-144 (2014). HPTLC of andrographolide (1), neoandrographolide (2) and deoxyandrographiside (3) in the aerial parts of Andrographis paniculata on silica gel with dichloromethane - toluene - ethanol 13:5:3. Detection by dipping into p-anisaldehyde–sulfuric acid reagent, followed by heating at 110 ºC for 6 min. For quantification, image analysis with an office scanner at 200 dpi and densitometric measurement in absorbance mode at 540 nm. The hRF values for (1) to (3) were 61, 35 and 16, respectively. Linearity was in the range of 3-24 μg/zone for (1) and 1-8 μg/zone for (2) and (3). The intermediate/interday/intra-day precisions were below 3 % (n=3). The LOD and LOQ were 510 and 1700 ng/zone for (1), 140 and 480 ng/zone for (2) and 6 and 190 ng/zone for (3), respectively. Recovery was between 96.1 and 104.3 % for (1) to (3). Comparable results were obtained with the TLC-image analysis method, TLC-densitomety and HPLC.

J. Liq. Chromatogr. Relat. Technol. 37, 2420-2432 (2014). HPTLC of lamivudine (1) and zidovudine (2) on silica gel with ethyl acetate – hexane – methanol – acetic acid 40:40:20:1. Quantitative determination by absorbance measurement at 278 nm. The hRF values for (1) and (2) were 25 and 73, respectively. Linearity was in the range of 100-600 ng/zone for (1) and 200-1200 ng/zone for (2). The intermediate/interday/intra-day precisions were below 2 % (n=3). The LODs and LOQs were 5 and 15 ng/zone for (1) and 10 and 32 ng/zone for (2), respectively. Recovery was between 95 and 105 %.

Trends in Chromatography 8, 131-135 (2013). Based on the Minilab TLC screening methods two individual and one simultaneous HPTLC methods for quantification of lumefantrine and artemether were developed. HPTLC of artemether, lumefantrine, and both substances in a combined tablet formulation on silica gel with ethyl acetate - glacial acetic acid - toluene 2:1:9 with chamber saturation. Quantitative absorbance measurement of lumefantrine at 254 nm. For artemether, detection by spraying with methanol - 96 % sulfuric acid 19:1 and heating at 100 °C for 5 minutes, evaluation in daylight and quantitative absorbance measurement at 610 nm. With the simultaneous method artemether zones were detected by heating at 160 °C for 5 min, and after cooling absorbance measurement at 254 nm was performed. This is a novel, reagent-free detection method based on thermochemical activation of fluorescence quenching. Precision (%RSD) for the artemether individual method was 0.1-0.6 % and recovery 98-103 %. Precision (%RSD) for the lumefantrine individual method was 0.9-1.8 % and recovery 100-102 %. With the simultaneous method the hRF was 34 for lumefantrine and 61 for artemether, precision (%RSD) was below 3 % and recovery was 98-100 %.

J. Planar Chromatogr. 27, 287-293 (2014). HPTLC of ciprofloxacin HCl (1) and moxifloxacin HCl (2) in industrial wastewater on silica gel with methanol - ammonia - methylene chloride 11:7:4. Quantitative determination by absorbance measurement at 278 nm. The hRF values for (1) and (2) were 43 and 54, respectively. Linearity was in the range of 250-2500 ng/zone for (1) and 1000-50000 ng/zone for (2). The intermediate/interday/intra-day precisions were below 1 % (n=3). The LOD and LOQ were 110 ad 335 ng/zone for (1) and 101 and 305 ng/zone for (2), respectively. Recoveries were between 79.6 and 91.5 % for both (1) and (2). Results were comparable to a HPLC method.

Food Chem. 176, 91-98 (2015). HPTLC of (1) mangiferin and (2) lupeol in the fruit of Mangifera indica on silica gel on a uni-dimensional double development with toluene - ethyl acetate - methanol 7:2:1 and ethyl - acetate - methanol 3:2. Detection of (2) by spraying with vanillin–sulphuric acid reagent (1 g vanillin - 5 mL sulphuric acid - 95 mL ethanol ). Quantitative determination by absorbance measurement at 390 nm for (1) and 610 nm for (2). The hRF values of (1) and (2) were 60 and 88, respectively. Linearity was between 250 and 2500 ng/zone for (1) and (2). The intermediate intra-day and inter-day precision were below 2 % (n=5) for (1) and (2). The LOD and LOQ were 258 and 832 ng/zone for (1) and 277 and 922 ng/zone for (2). Average recoveries for (1) and (2) were 98.1 and 99.2 %.

J. Planar Chromatogr. 28, 83-89 (2015). HPTLC of irbesartan in tablets and plasma on silica gel with acetonitrile - chloroform - glacial acetic acid 30:19:1. Detection by exposure to concentrated hydrochloric acid vapor for 10 min. Quantitative determination by fluorescene measurement at 255/>400 nm. The hRF value of irbesartan was 40. Linearity was between 7 and 90 ng/zone. The intermediate intra-day and inter-day precisions were below 2.8 % (n=6). The LOD and LOQ were 2 and 6 ng/zone, respectively. Recoveries were in the range of 96.9-98.6 % in tablets and 95.8-97.4 % in plasma.

Chinese J. Inform. Trad. Chinese Med. 20 (5), 67-69 (2013). Yinao Huoxue Keli granule is a TCM compound for the treatment of hemiplegia, numbness of limbs, deviation of mouth and tongue. For quality control, HPTLC on silica gel (1) for Salvia miltiorrhiza Bunge with chloroform – acetone – formic acid 8:1:1, detection by spraying with 3 % ferric chloride in 2 N hydrochloric acid – water 1:100 and heating at 105 °C until the spots were visible, identification by fingerprint comparison with the standard protocatechuic aldehyde and the standard ingredient drug; (2) for Panax quinquefolius L., ginsenoside Re, Rb1 and pseudoginsenoside F11 with the lower phase of chloroform – ethyl acetate – methanol – water 15:40:22:10 placed at 5-10 °C for 12 h, detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 °C until the spots were visible.

J. Planar Chromatogr. 28, 256-261 (2015). HPTLC of picroside-I (1) and picroside-II (2) in Picrorhiza kurroa on silica gel with ethyl acetate - methanol - acetic acid 80:10:1. Detection by dipping into anisaldehyde - sulfuric acid, followed by heating at 120 °C for 5 min. Quantitative fluorescence measurement at UV 366 nm. The hRF values for (1) and (2) were 57 and 66, respectively. Linearity was in the range of 200-4000 ng/zone. LOD and LOQ were 16 and 47 ng/zone for (1) and 16-48 ng/zone for (2). The intermediate precision was below 11 % (n=3).