J. Planar Chromatogr. 22, 283-286 (2009). HPTLC of withaferin and extracts of the powdered root on silica gel, prewashed with methanol, with toluene - ethyl acetate - acetone 2:3:3 in a twin trough chamber saturated with mobile phase for 30 min. Detection by spraying with anisaldehyde reagent followed by heating for 15 min at 105 °C; characteristic orange fluorescence was observed for whithaferin. Quantitative determination by absorbance measurement at 214 nm. The limit of detection and quantification for withaferin A was 258 and 782 ng/zone, respectively.

J. Planar Chromatogr. 22, 245-348 (2009). HPTLC of doxofylline (7-(1,3-dioxalan-2-ylmethyl)theophylline) in bulk drug and in formulations on silica gel, prewashed with methanol, with toluene - methanol 4:1 in a twin trough chamber saturated for 20 min. Quantitative determination by absorbance measurement at 275 nm.

Abstract No. F-275, 61st IPC (2009). HPTLC of ivabradine HCl on silica gel with methanol - chloroform 1:1. The hRf value was 59. Quantitative determination by absorbance measurement at 285 nm. Linearity was in the range of 100-800 ng/spot with r2=0.9989 (via peak area).

Abstract No. 9933, IHCB (2009). HPTLC of colchicines in Gloriosa superba (collected from different parts of India) on silica gel with ethyl acetate - methanol 200:27. The hRf value of colchicine was 29. Quantitative determination by absorbance measurement at 350 nm. The method was linear in the range of 50-1000 ng/spot. The sample collected from Kerala was found to contain highest level of colchicines (0.24 %).

Chinese J. Hospit. Pharm. 29 (4), 336-338 (2009). TLC of astragaloside in Tianjian capsules on silica gel with chloroform - methanol - water 13:6:2. Detection by spraying with 5 % sulfuric acid in ethanol followed by heating at 105 ºC until coloration. Quantification by densitometry at 515 nm. The linearity was between 0.98 and 4.90 µg/spot (r2 = 0.998), the %RSD was 2.4 % (n = 6) within plate and 2.3 % (n = 6) inter-plate, standard addition recovery was 99.2 % with RSD = 1.7 % (n = 6).

J. Liq. Chromatogr. Relat. Technol. 31, 2673-2685 (2008). TLC of dehydroepiandrosterone on silica gel with chloroform - acetone 17:3 with chamber saturation for 30 min. Detection by dipping into 0.05 % aqueous solutions of visualizing agents (methylene violet, gentian violet, janus blue, methylene blue, malachite green, rhodamine B) and methanolic chloramine T/methanolic sulfuric acid solution. Quantitative determination by densitometry at 200 nm (before derivatization and for methylene violet), 657 nm (gentian violet), 487 nm (janus blue), 488 nm (malachite green), 580 nm (rhodamine B), and 361 nm (chloramine T). LOD was 0.29 µg/zone (without visualizing agent, with chloramine T and with rhodamine B), 4.19 µg/ zone with methylene violet, 1.10 µg/zone with gentian violet, 2.14 µg/zone with janus blue, and 4.19 µg/zone with malachite green, all with an application volume of 5 µL.

J. Planar Chromatogr. 23, 35-39 (2010). HPTLC of ß-carotene on silica gel (prewashed with methanol) with petroleum ether - hexane - acetone 2:3:1 in a saturated twin-trough chamber. Quantitative determination by absorbance measurement at 450 nm. Linearity was between 100 and 600 ng/band. LOD and LOQ were 0.11 and 0.37 ng/band, respectively. Average intra-day precision and inter-day-precision were 0.54 % and 0.50 %, respectively.

Appl. Surf. Sci. 256, 6489-6494 (2010). TLC of naproxen released from a SBA-15 mesoporous silica drug delivery system on silica gel with benzene - tetrachloromethane - acetic acid 7:1:1. Quantitative determination by absorbance measurement at 260 nm at different time intervals. The hRf value of naproxen was 50.