Biomed. Chromatogr. 21 (10), 1064-1068 (2007). Indirect chiral separation of penicillamine (3,3-dimethylcysteine) enantiomers after derivatization with Marfey's reagent (FDNP-Ala-NH2) and two of its structural variants, FDNP-Phe-NH2 and FDNP-Val-NH2, with phenol - water 3:1 and solvent combinations of acetonitrile and triethylamine phosphate buffer in normal and reversed-phase TLC, respectively. Also separation of the diastereomers on a reversed-phase HPLC column with gradient elution of acetonitrile and 0.01 m trifluoroacetic acid. Comparison of the results due to these three reagents. Successful application of the method for checking the enantiomeric impurity of l-penicillamine in d-penicillamine and to check the enantiomeric purity of pharmaceutical formulations of d-penicillamine.

Food Chem. 77, 263-265 (2002). HPTLC of trehalose on silica gel, impregnated with phosphotungstic acid of pH 2.5, with n-butanol – pyridine – water 8:4:3. Detection by spraying with a solution of 6.5 mM N-(1-naphthyl)-ethylenediamine dihydrochloride in methanol, containing 3 % sulfuric acid. The hRf values of raffinose, trehalose, maltose, sucrose, glucose, and fructose were 30, 41, 46, 53, 55, and 59, respectively.

Food Chem. 95, 58-64 (2006). HPTLC of nicotinic acid (1) and pyrazole-3(5)-carboxylic acid (2) of Volvariella volvacea on silica gel with chloroform – methanol 17:3 with one drop of formic acid added. Quantitative determination by absorbance measurement at 190 nm for (1) and 262 nm for (2). The hRf values for (1) and (2) were 30 and 40, respectively. Linearity was between 400 and 7000 ng/zone (1) and 200 and 2500 ng/zone for (2). The limits of detection and quantification were 50 and 400 ng/zone for (1) and 20 and 200 ng/zone for (2). Recoveries of both compounds were between 96 and 102 %.

59th Indian Pharmaceutical congress E-243, 283, (2007). HPTLC of Bergenia ciliata leaves and rhizomes successively (Soxhlet) extracted with petroleum ether (40-60°C), chloroform, n-butanol, and ethyl acetate, on silica gel with ethyl acetate - glacial acetic acid - formic acid - water 128:50:50:122. Evaluation under UV 254 nm.

J. Planar Chromatogr. 20, 183-187 (2007). HPTLC of stigmasterol on silica gel prewashed with methanol in a saturated twin-trough chamber with chloroform - ethanol - formic acid 98:2:1. Detection by dipping in a 5 % methanolic sulfuric acid solution and heating at 105 °C for 5 min. Quantitation by scanning at 580 nm. Also TLC of dl-apha-tocopherol acetate on silica gel with cyclohexane - diethylether 9:1. Scanning at 200 nm.

J. Sep. Sci. 30, 2167-2172 (2007). HPTLC of clozapine in human serum on silica gel with chloroform - methanol 9:1. Quantitative determination by absorbance measurement at 290 nm. Linearity was between 10 and 100 ng/zone. The intra-assay variation was between 2.10 and 3.33 % (n=5) and inter-assay variation was between 2.67 and 4.44 % (n=9). The limits of detection and quantification were 0.03 and 0.05 ng/µL, respectively. Recovery was between 97.0 and 99.0 %, and selectivity regarding matrix was given.

59th Indian Pharmaceutical congress C-344, 308, (2007). TLC of different fractions of fruits of Azadirecta indica on silica gel with ethyl acetate - n-hexane 1:1. The MIC value was determined in comparison with gedunin isolated from the plant. The fungi were sub cultured and incubated for seven days, then sprayed on the developed plate, followed by incubation at 37°C . Zones of inhibition were measured. Antifungal activity was observed with Aspergillus niger, Fusarium specis, and Penicillium chrysogenum.

59th Indian Pharmaceutical congress F-10, 392, (2007). HPTLC of olmesartan medoxomil and hydrochlorothiazide on silica gel with acetonitrile - chloroform - glacial acetic acid 14:4:1 with chamber saturation for 30 min. Densitometric evaluation at 254 nm. Linearity was between 480 and 900 ng/zone for olmesartan and 150 and 600 ng/zone for hydrochlorothiazide. Recovery was 99.7 - 100.4 % . The method can be used for routine quality control of formulations.