CBS 90, 2-4 (2003). HPTLC-AMD on silica gel with a 11-step gradient with chloroform - ethanol - acetone followed by 3 isocratic steps with chloroform for separation of cholesterol, cholesterol sulfate and various ceramide classes. For separation of cholesterol, fatty acids, triacylglycerol, cholesteryl esters, and squalene a 2-step gradient with n-hexane - ethyl acetate followed by an isocratic step with n-hexane. Conditioning between single runs with 4 M acetic acid. Detection by dipping in copper sulfate reagent followed by heating at 150 °C for 20 min. Quantitative determination by absorbance measurement at 546 nm.

CBS 90, 14 (2003). HPTLC of waste water on silica gel with dichloromethane - methanol - ammonia 90:10:1.Quantitative determination by absorbance measurement at 270 nm.

J. Chinese Trad. Patent Med. (Zhongchengyao) 25 (9), 715-718 (2004). TLC on silica gel with 1) ethyl acetate - butanone - formic acid - water 5:3:1:1, 2) benzene - ethyl acetate - methanol - isopropanol - conc. ammonia 12:6:3:3:1, 3) chloroform - acetone 19:1, 4) n-butanol - glacial acetic acid - water 7:1:2. Detection 1) by spraying with 5 % FeCI3 in ethanol, 2) spraying with 5 % vanillin - H2SO4 solution and heating at 100 ºC for 10 min, 3) under UV 365 nm. Identification by fingerprint techniques. Quantification of berberine hydrochloride by densitometry at 340 nm.

Chinese J. Pharm. Anal. (Yaowu Fenxi Zazhi) 24 (1), 30-34 (2004). TLC on silica gel with chloroform - acetone - diethylamine 5:4:1. Detection under UV 254 nm. Identification by standard comparison. Use of silver gel as the surface enhanced subtract placed on the separated spots for measurement of FT-SERS. Investigation of the minimum detection amount for the compound, and the difference between the SERS and solid spectra.

Chemistry of Plant Protection 12, 181-195 (1995). AMD as a TLC technique, which combines the wide range of polarity gradient elution on silica with a separation power similar to HPLC. Application to parallel analysis of 12 samples for some dozens of pesticides. Limit of quantification is 0.05 µg active ingredient per liter. First step allows screening for active ingredients, second step confirms positive results by gradient separation and comparison of UV spectra of sample and standard. Offline-coupling with MS. Standardization as DIN method 38407-part 11 for determination of pesticides in ground and drinking water.

Chinese J. Chromatogr. (Sepu) 22 (3), 296-297 (2004). TLC of rhodojaponin III on silica gel with chloroform - methanol 9:1. Detection by spraying with 10 % H2SO4 in ethanol and heating. Identification by comparison with the standard. Quantification by densitometry at 550 nm. Validation of the method by investigating its linearity range (0.1-4.0 µg/spot, r=0.997), precision (RSD=1.30-3.34 %, n=8), and recovery (95.4-101.5 %, RSD=1.97 %, n=5).

Chinese J. Trad. Patent Med. (Zhongchengyao) 26 (7), 541-544 (2004). TLC of Fuketiaojing tablet extracts on silica gel with 1) benzene - ethyl acetate - glacial acetic acid 2:1:1; 2) benzene - ethyl acetate- glacial acetic acid 92:5:5; 3) cyclo hexane - ethyl acetate 7:3; 4) chloroform - methanol - water 30:10:1. Detection 1) under UV 254 nm; 2) by spraying with p-dimethylaminobenzaldehyde-H2SO4 solution and heating at 130 ºC and under UV 365 nm; 3) by spraying with 10 % H2SO4 solution in ethanol and heating at 105 ºC; 4) by spraying with 5 % vanillin-H2SO4 solution and heating at 105 ºC. Identification by fingerprint technique. Quantification of ferulic acid by HPLC.

Chinese J. Trad. Patent Med. (Zhongchengyao) 26 (4), Appendix 7-8 (2004). TLC of Xuanmuyi capsule extracts on silica gel with n-butanol - hydrochloric acid - water 8:2:10:1. Detection by spraying with 5 % potassium iodobismuthate. Identification by fingerprint technique. Quantification by densitometry at 510 nm. Validation of the method by investigation of its linearity range (10-50 µg/spot, r=0.9999), precision (RSD=2.1 %, n=6), standard addition recovery (100.7 %, RSD=3.8 %, n= 5).