Abstract G-26, IPC (2005). A simple HPTLC method is reported for analysis of guggulsterones E and Z in herbal extract and market formulations containing commiphora mukul. Guggulsterones were extracted from crude extract and formulations by ethyl acetate. HPTLC on silica gel with n-hexane - ethylacetate 3:1. Quantitative determination by absorbance measurement at 250 nm. The hRf value of E-guggulsterone was 38 and of Z-guggulsterone 46, linearity range for both isomers was 200-5000 ng/mL. The method was validated as per ICH guidelines.

Abstract GP-18, IPC (2005). HPTLC of nimesulide and chlorzoxazone in tablets on silica gel (prewashed with methanol) with toluene - acetone - ammonia 50:50:4. Paracetamol was used as internal standard. Quantitative determination by absorbance measurement at 265 nm. Nimesulide, chlorzoxazone and paracetamol showed hRf values of 80, 73 and 42, respectively. Linearity was obtained between 0.2-1.0 mg/mL with recovery rates of 99.6-100.3 % for both compounds.The method was validated for accuracy, precision, linearity, LOD, and LOQ.

Abstract D-12, IPC (2005). HPTLC of methanol and ethyl acetate extracts of trigonella foenum graecum on silica gel with n-propanol - ammonia 11:9. Detection by spraying with ninhydrin reagent. Quantitative determination by absorbance measurement and in visible range. The hRf value of isoleucin was 60. Methanolic extracts contained 0.17 % isoleucin and ethyl acetate extracts 0.008 %. Accuracy, precision, linearity of the method were established.

Indian J. Pharm Sciences 67 (6), 687-690 (2005). HPTLC of sparfloxacin extracted with dichloromethane from plasma. A standard solution was prepared in methanol - dichloromethane 1:1. HPTLC on silica gel with chloroform - toluene - methanol - diethylamine 44:15:2:1. Quantitative determination by absorbance measurement at 301 nm. The method had a linearity range of 80-200 ng/spot with an average recovery of 89.17 %.

CBS 96, 6-7 (2006). HPTLC of oxytetracycline in salmon feed, on silica gel (pre-washed with methanol and dried at 120 °C for 30 min, followed by dipping into 5 % EDTA solution of pH 7.0 and drying at 120 °C for 1 h in an oven) with the organic layer of dichloromethane - methanol - 5 % EDTA 13:4:2 with chamber saturation for 30 min.Quantitative determination by fluorescence measurement at UV 366/>400 nm. Calibration (peak area) was performed via linear regression with r2 of 0.9925. Recovery rates for oxytetracycline at 500, 2500, and 5000 mg/kg were 73 ± 4.2 %, 101 ± 2.6 %, and 101 ± 4.0 %. Intermediate precisions at the same levels were 5.7, 2.6 and 4.0 %. At an application volume of 10 µL LOD was 14.8 mg/kg (n=3) and LOQ was 49.2 mg/kg (n=10). Quantification was achieved between 100 and 10000 mg/kg oxytetracycline in salmon feed due to the selectivity of fluorescence measurement.

J. Pharmaceutical Research 4 (3), 55-57 (2005). HPTLC of indapamide on silica gel with toluene - methanol 7:3. Quantitative determination by absorbance measurement at 246 nm. The method was linear within the range of 1.4-3.7 µg/mL. Mean recovery values were 99.46-100.29 %. The method was validated for accuracy, precision, and specificity.

Indian Drugs 42 (10), 665-670 (2005). HPTLC of carbamazepine, phenytoin, and phenobarbitone extracted with ethyl acetate from human serum, on silica gel with toluene - acetone 5:2. Quantification by absorbance measurement at 217 nm. Rf values were 0.20 for carbamazepine, 0.41 for phenytoin, and 0.49 for phenobarbitone. The linearity (r=0.998) was in the range of 100-2000 ng. LOQ was found to be 30 ng/spot for carbamazepine and 80 ng/spot for phenytoin and phenobarbitone. The accuracy was in the range of 88.5 to 98.1 % and the CV in range of 1.1 to 3.9 %. Intra day and inter day reproducibility was comparable and within the stated limits.

J. Planar Chromatogr. 19, 348-354 (2006). HPTLC of puerarin, daidzein, daidzin, and 3’-methoxypuerarin on silica gel, pre-washed with methanol, in an unsaturated twin-trough chamber with chloroform - methanol - ethyl acetate - water 16.2:18.8:52:3. Quantitative determination by absorbance measurement at 254 nm. The relative standard deviation of Rf values, retention times and peak area percentages all meet the national standards.