Cumulative CAMAG Bibliography Service CCBS
Our CCBS database includes more than 11,000 abstracts of publications. Perform your own detailed search of TLC/HPTLC literature and find relevant information.
The Cumulative CAMAG Bibliography Service CCBS contains all abstracts of CBS issues beginning with CBS 51. The database is updated after the publication of every other CBS edition. Currently the Cumulative CAMAG Bibliography Service includes more than 11'000 abstracts of publications between 1983 and today. With the online version you can perform your own detailed TLC/HPTLC literature search:
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J. Planar Chromatogr. 24, 257-263 (2011). HPTLC of hydrophilic and lipophilic constituents of Salvia miltiorrhiza and standards protocatechuic acid and aldehyde, salvianolic acid A and B, dihydrotanshinone I, rosmarinic acid, caffeic acid, cryptotanshinone, tanshinone II A, tanshinone I, and miltirone on silica gel with dichloromethane - ethyl acetate - formic acid 11:12:5 for the first development and petroleum ether - ethyl acetate - cyclohexane 15:11:14 for the second development with chamber saturation for 30 min. The first mobile phase separated the hydrophilic constituents salvianolic acid B, salvianolic acid A, rosmarinic acid, caffeic acid, protocatechuic acid, and protocatechuic aldehyde. Detection under UV light at 254 and 365 nm. After documentation the plates were placed in a second chamber and development with the low polarity mobile phase which separated dihydrotanshinone I, cryptotanshinone, tanshinone I and II A, and miltirone. Detection under UV light at 254 and 365 nm. Quantitative determination by densitometry in absorbance mode at 260 or 290 nm. The linear range was between 0.1-0.3 and 0.7-8.3 µg/zone. Instrumental precision was less than 4 % (n = 6). Precision on one plate was below 5 % (n = 6) and on different plates below 14 %. Depending on the substance, the limits of detection and quantification were between 14-22 and 69-276 ng/zone, respectively. The repeatability (n = 6) was between 1.3-3.4 %. Some of the compounds had similar hRf values: for rosmarinic acid 44, for salvianolic acid 43, for caffeic acid 49, for protocatechuic acid 49, for dihydrotanshinone 65 and for cryptotanshinone 63. Additional detection by spraying with 5 % sulfuric acid in ethanol.
J. Planar Chromatogr. 24, 482-486 (2011). HPTLC of (-)-epicatechin and procyanidin B2 in chocolates on cellulose with n-propanol - water - acetic acid 20:80:1. Detection by immersion for 1 s in 4-dimethylaminocinnamaldehyde. Quantitative determination by densitometry at 655 nm. The samples contained 13 mg/100 g each of (-)-epicatechin and procyanidin B2 with a relative standard deviation of 5.8 and 4.2 % (n = 6), respectively. The calibration curves were polynomial in the range of 2-30 ng/zone for (-)-epicatechin and 4-60 ng/zone for procyanidin B2. LOD was 0.2 ng/zone (0.7 pmol) and 2 ng/zone (3.5 pmol) as well as LOQ was 0.4 ng/zone (1.4 pmol) and 4 ng/zone (7 pmol) for (-)-epicatechin and procyanidin, respectively.
J. Planar Chromatogr. 24, 474-481 (2011). TLC of the active S-(+)-enantiomer escitalopram oxalate (ESC-OX), escitalopram cyanodiol, the R-enantiomer and escitalopram N-oxide impurities on silica gel (containing beta-cyclodextrin as chiral additive) with acetonitrile - 0.1 % acetic acid - water 10:1:6:2 with chamber saturation for 30 min. Using 3 mg urea per 100 cm2 of silica-coated plates as a chiral additive also achieves a good enantiomeric separation with acetonitrile - 1 % acetic acid - ethyl acetate - methanol - water 10:1:2:4:3. Detection at 254 nm. Quantitative determination by absorbance measurement of ESC-OX at 240 nm. The hRf values of ESC-OX, escitalopram cyanodiol, the R-enantiomer and escitalopram N-oxide were 75, 40, 31, and 23, respectively. The linearity was 0.25-10 mg/10 mL (r = 0.9991). Accuracy was 99.7 %. LOD and LOQ were 13 and 44 µg/mL for ESC-OX.
J. Planar Chromatogr. 24, 534-538 (2011). HPTLC of clarithromycin in plasma on silica gel (prewashed with methanol) with ethyl acetate - methanol - 15 % ammonium acetate (pH 10.6) 7:2:1 in a twin-trough chamber with saturation for 15 min. Detection by dipping into xanthydrol solution (10 % in methanol). Quantitative determination by densitometry in absorbance mode at 506 nm. The hRf was 62. The method was linear over the range of 0.1-3.0 µg/mL (r2 = 0.9974). The recovery (by standard addition) was over 85 %. The intra-day and inter-day precision (%RSD) of the assay was in the range of 0.8-4.6 %. The recovery was above 95 %.
J. Planar Chromatogr. 24, 218-221 (2011). HPTLC of alfuzosin hydrochloride (ALF) and dutasteride (DUTA) in the bulk drug and in a tablet formulation on silica gel with toluene - methanol - dichloromethane 6:1:1 + 1 drop triethylamine. Quantitative determination by densitometry at 247 nm. The hRf of ALF was 46 and of DUTA 65. Linearity was between 300-600 ng/band for ALF and 500-100 ng/band for DUTA. LOD and LOQ were 100 and 200 ng/band for ALF and 300 and 400 ng/band for DUTA. Precisions (%RSD) for repeatability of application were 1.8 and 1.5 % for ALF and 1.5 and 1.4 % for DUTA. The inter-day and intra-day precision (%RSD, n = 6) was 1.0 and 0.9 % for ALF and 1.7 and 0.8 % for DUTA, respectively. Recovery (by standard addition) was between 98.9-101.6 % for both compounds.
Food Chemistry 132, 549-553 (2012).. New HPTLC-based method to examine quantitatively the free radical scavenging activity of plant extracts. After chromatographic separation of polar compounds, and immersion of HPTLC plates in methanolic DPPH radical reagent, bleaching was observed and recorded using a photo camera and data analysis was carried out using an image processing software. The method is simple, fast and efficient for free-radical scavenging activity analysis of phytochemicals and crude plant extracts.
J. Liq. Chromatogr. Relat. Technol. 34, 1925-1937 (2011). HPTLC of p-hydroxy benzoic acid (1), chrysoplenol-D (2), p-methoxy benzoic acid (3) and casticin (4) in the aerial parts of Vitex trifolia on silica gel with chloroform - methanol 24:1 + 1 drop formic acid. Quantitative determination by absorbance measurement at 254 nm. The hRf values of compounds (1) - (4) were 25, 31, 63 and 87, respectively. LOD and LOQ were found to be 18-58 ng/zone for (1), 39-132 ng/zone for (2),15-52 ng/zone for (3) and 33-111 ng/zone for (4). Repeatability and reproducibility (%RSD) for (1)-(4) were found in the range of 0.8-1.2 % and 1.2-1.3 %, respectively. Recoveries were obtained in the range of 94.0-101.1 %, 97.8-102.0 %, 95.1-100.1 %, and 97.6-100.3 % for compounds (1) - (4), respectively.
J. Planar Chromatogr. 25, 314-319 (2012). HPTLC of 4-odemethylpodophyllotoxin (1), podophyllotoxin (2), kaempferol (3), podophyllotoxone (4), and deoxypodophyllotoxin (5) in the rhizomes of Podophyllum hexandrum on silica gel with toluene - ethyl acetate 2:1 + 1 drop glacial acetic acid. Quantitative determination by absorbance measurement at 254 nm. Linearity was in the range of 1-8 µg/band for (1), (2) and (4) and 2-10 µg/band for (3) and (5). The intermediate/inter-day/intra-day precision was below 2 %. Average recovery for all (1) to (4) were between 96.4 and 101.8 %.