J. Liq. Chromatogr. Relat. Technol. 34, 1783-1799 (2011). HPTLC of isotretinoin in topical formulations on silica gel with toluene - methanol 9:1. Quantitative determination by absorbance measurement at 340 nm. The hRf of glycyrrhizin was 43. Linearity was 100-500 ng/zone. LOD and LOQ were found to be 13 and 42 ng/zone. The intra-day and inter-day precision precisions (n = 5) were 1.8 % and 1.6 %, respectively. Recovery (by standard addition) ranged from 99.1-100.6 %.

J. Planar Chromatogr. 24, 428-434 (2011). HPTLC of 6-benzyl-, 6-methyl-, and 6-propyl-2-thiouracil and spiked urine samples on silica gel with methanol in a horizontal chamber saturated for 15 min at ambient temperature. Detection by spraying with a freshly prepared mixture of 4 % sodium azide and 1 % starch solution adjusted to pH 5.5, followed by exposure to iodine vapor for 5 s. Quantitative evaluation by use of an office scanner at 300 dpi resolution. The images were inverted and stored in the form of 24-bit-true color images, which were analysed by TLSee software. The determination range was 7-16 pmol/zone, 80-160 nmol/mL urine, or 133-266 nmol/mL serum. The recovery was between 93-106 %. The LOQ was 4 pmol/zone for the studied thiouracils in three investigated matrices.

J. Planar Chromatogr. 25, 331-337 (2012). HPTLC of danazol on silica gel with hexane - acetone 3:2. Quantitative determination by absorbance measurement at 291 nm. The hRf value of danazol was 55. Linearity was in the range of 200-1200 ng/zone. Limits of detection and quantification were 1 and 4 ng/zone.

J. Planar Chromatogr. 25, 374-379 (2012). HPTLC of atazanavir sulfate (1) and ritonavir (2) in combined dosage forms on silica gel with toluene - methanol - glacial acetic acid - ethyl acetate 14:1:3:4. Quantitative determination by absorbance measurement at 254 nm. The hRf values for (1) and (2) were 50 and 63, respectively. Linearity was in the range of 30-300 ng/zone for (1) and 10-100 ng/zone for (2). The limit of detection and quantification was 16 and 49 ng/zone for (1) and 18 and 55 ng/zone for (2), respectively. The intermediate/inter-day/intra-day precision was below 0.7 % (n=6). Recovery for (1) and (2) was in the range of 99.6-100.0 %.

Chinese J. of Inform. on TCM 17 (12), 48-50 (2010). Fuyanshuan suppository is a herbal TCM preparation for heat clearing, detoxifying, eliminating stagnation, regulating the flow of vital energy and invigorating the circulation of blood. For quality control TLC of the extracts of the preparation 1) for Ajuga becumbens Thunb., on silica gel with cyclohexane - chloroform – ethyl acetate – glacial acetic acid 40:10:16:1, detection by spraying with 10 % sulfuric acid in ethanol and heating at 100 °C, evaluation under UV 366 nm or daylight; 2) for Lonicera japonica, on silica gel with chloroform – acetone – formic acid 3:2:1, detection by exposure to daylight for 10 min and viewing under UV 366 nm.

& Wendl. (Solanaceae) against ethylene glycol induced urolithiasis in rats. J. Ethnopharmacol. 138, 160-170 (2012). HPTLC of solasodine in the fruits of Solanum xanthocarpum on silica gel with toluene - ethyl acetate - diethylamine 12:1:1. Quantitative determination by absorbance measurement at 200 nm. The hRf of solasodine was 52.

J. of World Trad. Chinese Med. 7 (1), 77-78 (2012). Yiqi Shenggu granule is a newly developed herbal TCM preparation for treating wounded catagma. TLC of the extracts of the preparation on silica gel 1) for Aucklandia root, with petroleum ether (60-90 ºC) – ethyl acetate 9:1, detection by spraying with 5 % vanillin in ethanol – sulfuric acid 200:1 and mild heating, viewing in daylight; 2) for Carthamus flower, twice with chloroform – toluene – methanol 15:5:1, detection by spraying with 10 % sulfuric acid in ethanol and viewing at UV 366 nm; 3) for red Peony root, with chloroform – ethyl acetate – methanol – formic acid 200:25:50:1, detection by spraying with 5 % vanillin in ethanol – sulfuric acid 200:1 and mild heating, viewing in daylight. Determination of astragaloside A in the extract of the medicine by TLC on silica gel with chloroform – methanol – water 13:7:2, detection by spraying with 10 % sulfuric acid in ethanol and viewing at UV 366 nm. Quantification by densitometry at 520 nm with a linearity range of the calibration curve of 1.13-6.75 µg/zone (r = 0.995, n = 6), a precision of %RSD = 2.0 (n = 6) within plate and plate-to-plate, and standard addition recovery of 99.4 % (%RSD = 2.3, n = 6).

J. Planar Chromatogr. 26, 119-124 (2013). HPTLC of methyl- (1), ethyl- (2), propyl- (3), and butylparaben (4) in cosmetics on cyanopropyl plates with water - acetonitrile - dioxane - ethanol 8:2:1:1+1 drop ammonia. Quantitative determination by absorbance measurement at 255 nm and by bioutographic analysis using Vibrio fischeri bacteria. LOD for (3) and (4) was 100 ng/zone, for (1) 80 ng/zone, and 69 ng/zone for (2). The LOQ for (3) and (4) was 120 ng/zone, for (1) 90 ng/zone, and 78 ng/zone for (2).