Acta Chromatographica 20(3), 283-307 (2008). TLC of twenty flavonoids and their analogues on different stationary phases (non-polar and polar bonded stationary phases, silica gel, amino phase, diol phase) developed with a variety of binary mobile phases (aqueous and non-aqueous). Evaluation of similarities and differences among the chromatographic systems by principal component analysis and hierarchical clustering. Application of scoring indices to the separation power of a given system or a pair of systems allowed selection of the most suitable systems either to perform two-dimensional separations or to enhance the overall resolution by merging two stationary phases. On the basis of the investigation relatively efficient two-dimensional system on amino phase were developed. TLC with tetrahydrofuran – water 9:1 in the first dimension and acetonitrile – water 9:1, 4:1, 3:1, or 7:3 in the second dimension was found to be suitable for the separation of the compounds. Theoretically the compounds were best separated by combining diol and amino phases and using methanol – water 3:2 and acetonitrile – water 9:1, respectively.

62nd Indian Pharmaceutical Congress Abstract No. F-246 (2010). TLC of atorvastatin calcium, ezetimibe and fenofibrate on silica gel with toluene – methanol – chloroform 6:3:4. Quantitative determination by absorbance measurement at 280 nm. The method was found to be linear in the range of 100-600 ng/band for atorvastatin calcium and ezetimibe and 50-300 ng/band for fenofibrate.

J. Pharm. Biomed. Anal. 54, 497-502 (2011). HPTLC of three bioactive steroidal glycoalkaloid markers, solasonine (SN), solamargine (SM) and khasianine (KN) in the plant Solanum xanthocarpum. The extraction effciency of targeted SGAs from plant matrix using methanol and acidified methanol were studied using percolation, ultrasonication and microwave techniques. HPTLC on silica gel with chloroform – methanol – water. The hRf values were 31, 37, and 52 for SN, SM, and KN, respectively. Quantitative determination by absorbance measurement at 520 nm after derivatization using Dragendorffs reagent. The linearity range was 2-10 µg/band for SN and SM and 6-30 µg/band for KN. Method specificity was confirmed using hRf values, correlation of UV spectra and comparison of ionization mass spectra (ESI-MS) of marker compounds in the sample track.

62nd Indian Pharmaceutical Congress Abstract No. F-236 (2010). TLC of rupatadine formulation on silica gel with acetonitrile – water – formic acid 50:50:3. The hRf value was 67. Quantitative determination by absorbance measurement at 263 nm. The method was linear in the range of 1-20 µg/band.

Modern J. of Integrated Trad. Chinese & Western Med. 19(31), 3439-3441 (2010). TLC on silica gel with chloroform – methanol – water 13:7:2. Detection by spraying with 10 % slfuric acid in ethanol and heating at 105 °C until the zones are visualized, evaluation under UV 366 nm. Identification of the component drugs Radix Astragali and Rhizoma Chuanxiong P.E by comparison of the retention values and color of the zones by the active compounds astragaloside and ferulic acid in the individual drug.

Chinese J. Mod. Drug Appl. 4(15), 16-17 (2010). TLC on silica gel 1) with n-butanol – glacial acetic acid – water 4:1:5 for Xanthium sibiricum Patr., detection by exposure to iodine vapors; 2) with trichloromethane – diethyl ether 5:1 for Flos magnoliae, detection by spraying with 10 % sulfuric acid in ethanol and heating at 90 °C until the zones were visualized; 3) with n-butanol – ethyl acetate 17:3 for herba Menthae, detection by spraying with vanillin reagent and heating at 105 °C until the zones were visible; 4) with petroleum ether (30-90 °C) – ethyl acetate 17:3 for Angelica dahurica (Fisch. ex Hoffm.) Benth. et Hook. f. ex Franch. et Sav., detection under UV 366 nm.

Chinese J. Ethnomed. Ethnopharm. (23), 61-64 (2010). Optimization of the sample preparation procedure for Herba Saururi chinensis 1) by ultrasonication with methanol for 60 min; 2) by ultrasonication with methanol for 20 min and filtration through neutral alumina column with methanol; 3) by reflux extraction with 80 % methanol for 60 min and extraction with diethyl ether; 4) by ultrasonication with ethanol for 60 min; 5) by ultrasonication with methanol - 25 % hydrochloric acid 4:1 for 60 min and extraction with ethyl acetate. Procedure 5) was best suited. TLC on silica gel 1) with petroleum ether (60-90 °C) – acetone 5:2, and detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 ºC until the zones appear; 2) with toluene – ethyl acetate – formic acid 5:2:1, and detection by spraying with 1 % AlCl3 in ethanol and evaluation under UV 366 nm; 3) with n-hexane – ethyl acetate – formic acid 70:50:8, and detection by spraying with 1 % AlCl3 in ethanol, heating at 105 ºC and evaluation under daylight or under UV 366 nm; 4) with toluene – ethyl acetate – formic acid 5:4:1 with chamber saturation with hydrochloric acid vapor, detection under daylight or UV 366 nm.

CBS 107, 9-10 (2011). Extraction of pesticides from fruit and vegetable samples by QuEChERs method. TLC of acetamiprid, azoxystrobin, chlorpyriofos, fenarimol, mepanipyrim, penconazole and pirimicarb on amino phase aluminum foil (prewashed with acetonitrile) with acetonitrile over a migration distance of 75 mm in the first direction. After drying development in the backwards direction over 45 mm with acetone. Evaluation under UV 254 nm, UV 366 nm, white light and under UV 366 nm after immersion in primuline solution. Extraction of the target zone by TLC-MS interface with acetonitrile - 10 mM ammonium formate 1:1. Average recoveries of the seven pesticides were 90-104 % with %RSD of 0.3-4.1 % (n = 5). This new high-throughput planar solid phase extraction method for multi-residue analysis of pesticides in food allows a rapid and efficient clean-up at low costs and low solvent consumption.