Abstract No. C-454, 61st IPC (2009). HPTLC of ursolic acid and luteolin from aerial parts of Lippia nodiflora on silica gel with toluene - ethyl acetate - formic acid 70:30:3. The hRf value of luteolin was 34 and of ursolic acid 85. Densitometric analysis at 254 nm for luteolin and at 530 nm for ursolic acid after derivatization with natural products reagent followed by PEG reagent. The recovery of both marker components was in the range of 98.6-100.5 %.

J. Sep. Sci. 33, 2619-2628 (2010). HPTLC of 33 newly synthesized arylpiperazines on RP-18 with 1) methanol - water (with increasing concentrations of methanol from 80-100 %), 2) dioxane - water (with increasing concentrations of dioxane from 60-80 %), and 3) dimethyl sulfoxide - water (with increasing concentrations of dimethyl sulfoxide from 80-100 %). In all cases the organic modifier was increased in steps of 5 %. Quantitative determination by absorbance measurement at 254 nm. A quantitative structure-retention relationship study allowed to understand the chromatographic behavior of similar compounds.

International Journal of ChemTech Research 1(4), 1079-1086 (2009). TLC of wedelolactone, quercetin, and jatamansone in a polyherbal formulation. The formulation (oil) was extracted with methanol, the supernatant was concentrated under vacuum and used for chromatographic analysis. TLC on silica gel with toluene - acetone - formic acid 11:6:1 for wedelolactone, toluene - ethyl acetate - methanol 44:50:6 for quercetin and petroleum ether - acetone 3:1 for jatamansone. The hRf value of jatamansone was 34, of wedelolactone 56, and of quercetin 47. Densitometric evaluation at 254 nm, 285 nm and 366 nm for quercetin, jatamansone, and wedelolactone respecticely. The method was linear in the range of 500-2500 ng/band for wedelolactone, 3000-8000 ng/band for quercetin and 2000-6000 ng/band for jatamansone. The polyherbal oil formulation contained 1.62 %, 0.24 % and 0.11 % of wedelolactone, quercetin, and jatamansone respectively.

Research J. Pharm. and Tech. 3(3), 825-827 (2010). TLC on silica gel (plates pre-washed with methanol) with chloroform - methanol 4:1. The hRf value of aceclofenac was 38 and of diacerein 66. Densitometric evaluation at 256 nm. The linearity range was 10-50 µg/band for aceclofenac and 5-25 µg/band for diacerein. The recovery was in the range of 99.3-101.8 % for both compounds. The method was suitable for routine quality control of combined dosage forms without any interference from the excipients.

Scholars Research Library 2(1), 489-494 (2010). HPTLC of montelukast sodium and levocetirizine dihydrochloride on silica gel with toluene - chloroform - methanol - glacial acetic acid 6:20:10:1 in a twin trough chamber with chamber saturation for 25 min. The hRf value of levocetirizine was 64 and of montelukast 89. Densitometric evaluation at 302 nm. The method was linear in the range of 200-3200 ng/band for montelukast and 400-1200 ng/band for levocetirizine. The recovery was 99.9-100.0 %.

International Journal of ChemTech Research 1(4), 1129-1135 (2009) The marker compound was first isolated by column chromatography over silica gel by elution with toluene - ethyl acetate 17:3. TLC of ethanolic extracts of Syzgium cumini seed on silica gel with toluene - ethyl acetate 17:3. The hRf value of the marker compound was 50. Densitometric evaluation in fluorescence mode at 366 nm. The method was linear in the range of 1-5 µg/band. The extract of the powdered sample contained 7.4 % of the marker compound.

International Journal of Pharma World Research 1(2) (2010). A validated stability indicating assay method is described for estimation of voglibose in bulk and dosage formulation. TLC on pre-washed silica gel plates with acetonitrile - methanol - 25 % ammonia 150:40:1. The hRf value of voglibose was 66. Densitometric evaluation at 284 nm. The method was linear in the range of 100-450 ng/band. The average recovery was 101.4 %.

Journal of Pharmacy Research 2(1), 189-195 (2009). A validated HPTLC method has been developed for simultaneous estimation of valdecoxib and tizanidine in dosage form. HPTLC on silica gel with toluene - methanol - ethyl acetate 2:2:1. The compounds were well separated as compact bands. The method was linear in the range of 10-100 ng/band for tizanidine and 100-1000 ng/band for valdecoxib. The recovery was in the range of 99.3-101.2 %. Densitometric quantification at 254 nm. The method is suitable for quality control of combined dosage form.