J. Planar Chromatogr. 18, 319-322 (2005). HPTLC of emodin and phenolic acids (protocatechuic, homoprotocatechuic, caffeic, syringic, vanillic, ferulic, p-hydroxyphenylacetic, alpha-resorcylic, p-coumaric, gallic and ellagic acid) on silica gel in horizontal chambers with toluene - dichloromethane - ethyl acetate 4:4:1. Also two-dimensional TLC of phenolic acids on cellulose with benzene - methanol - acetic acid - acetonitrile 16:2:1:1 in the first direction and sodium formate - formic acid - water 10:1:200 in the second direction. After drying the chromatograms were observed under UV light at 254 nm before and after treatment with ammonia vapor. Derivatization was performed by spraying with either diazotized sulfanilic acid in 20 % sodium carbonate solution or with 2 % aqueous iron(III) chloride. Detection limits between 10 and 64 ng. Videodocumentation and quantitation by densitometry.

J. AOAC Int. 88, 1549-1554 (2005). TLC of polymyxin B, framycetin, and dexamethasone on silica gel with methanol and methanol - n-butanol - 25 % ammonia - chloroform 14:4:9:12 for framycetin and polymyxin B. Quantitative determination by densitometry at 550 nm after detection with 0.3 % ninhydrin solution. Dexamethasone was separated with cyclohexane - ethyl acetate 2:3, quantitative determination by absorbance measurement at 245 nm. Similar accuracy, relative standard deviation values from 1.49 to 2.47 % and relative error values from 0.02 to 0.81 % are comparable to those obtained with the reference methods.

Abstract G-5, IPC (2005). HPTLC of telmisartan and hydrochlorothiazide on silica gel with chloroform - methanol - toluene 2:5:5 with chamber saturation for 30 min. Detection by spraying with ninhydrin reagent. Quantitative determination by absorbance measurement at 272 nm. The linearity was in the range of 250-500 ng/spot for telmisartan and 200-700 ng/spot for hydrochlorothiazide, recovery was 99-101 %. Accuracy, precision and linearity of the method were established.

Chinese J. Chromatogr. (Sepu) 23 (5), 555-561 (2005). Use of TLC in combination of spectrophotometry and titrimetry to evaluate chromatographic characteristics of bauxite constituents. Examination of the retention behaviors of four constituents (Al³+, Fe²+, Ti4+, Si4+) on modified silica gel and cellulose with a mobile phase containing aqueous sodium chloride, formic acid and hydrochloric acid. Ternary separation of Al-Fe-Ti was achieved on silica gel H. Study of pH influence, presence of impurity elements in the samples, nature of the stationary phase on the ternary separation, and of detection limits of bauxite constituents; detection of silica in bauxite on cellulose plates. Quantitative determination of Al³+, Fe²+, and Ti4+ on silica gel H impregnated with sodium formate.

III. Separation on various stationary phases. J. Liq. Chrom. & Rel. Technol. 27, 2613-2623 (2004). TLC of cholic, glycocholic, glycolithocholic, deoxycholic, chenodeoxycholic, glycodeoxycholic, and lithocholic acid on different preparations of silica gel and a mixture of silica gel and Kieselguhr with n-hexane - ethyl acetate - acetic acid in various volume compositions; successful separation was achieved with the compositions 4:4:1 and 22:21:5. Detection by 10 % aqueous sulfuric acid followed by heating at 120 °C for 20 min. Densitometric measurement at 250 nm.

J. Chinese Trad. Patent Med. (Zhongchengyao) 27 (7), 786-790 (2005). TLC of Hainan holly on silica gel with 1) ethyl acetate - butanone - formic acid - water 5:3:1:1; 2) ethyl acetate - formic acid - water 14:5:5; 3) benzene - acetone - methanol - formic acid 85:15:10:2. Detection 1) by spraying with 1 % AlCl3 solution; 2) under UV 365 nm; 3) by spraying with 5 % phosphomolybdic acid in ethanol followed by heating at 105 ºC until the spots are visualized. Identification by fingerprint techniques. Quantification of rutin by HPLC. Discussion of the optimum processing procedure by comparison of TLC fingerprints and HPLC results of rutin content.

J. Chinese Trad. Patent Med. (Zhongchengyao) 27 (7), 859-861 (2005). TLC of Ganlu Xiaodu capsules on silica gel with chloroform - methanol - water 185:15:2. Detection under UV 254 nm. Identification by fingerprint techniques and by HPLC. Quantification of scutellarin by HPLC.

et Zucc by thin-layer chromatography) (Chinese). J. Chinese Trad. Patent Med. (Zhongchengyao) 27 (5), 605-607 (2005). TLC of resveratrol on silica gel with petroleum ether (30 ºC-90 ºC) - ethyl acetate - methanol - glacial acetic acid 200:50:35:1. Detection under UV light. Identification by comparison with the standard. Quantification by densitometry at 293 nm. Validation of the method by investigation of its linearity range (0.5 µg - 5.0 µg, r = 0.999); precision (RSD = 0.98 %, n = 5); its reproducibility of five time assay towards the same sample (RSD = 2.52 %); standard addition recovery (101.6 %, RSD = 0.26 %, n = 5). The results for three real life samples are given. Discussion of the application of the procedures for the quality control of the medicine.