J. Planar Chromatogr. 29, 59-65 (2016). HPTLC of methadone in syrup on silica gel (1) or RP-18 (2) with methanol – 25 % ammonia 10:1. Detection by spraying with 1 mL of Dragendorff reagent. Quantitative determination using a flatbed scanner with a resolution of 16 bits. The hRF of methadone was 44 on (1) and 23 on (2). Linearity was in the range from 60 to 1260 ng/zone. The LOD and LOQ were 100 and 200 ng/zone, respectively. A total relative uncertainty of 6.5 % was achieved.

Chinese J. of Med. Guide 12 (10), 8-10 (2014). Huayu Tongluo Jianpan Tie plaster is coated with a herbal TCM preparation for the treatment of pain and numbness in the neck, waist, upper limbs and legs caused by the protrusion of cervical and lumbar intervertebral discs. For quality control, TLC on silica gel (1) for Boswellia carterii Birdw./Boswellia bhaurdajiana Birdw. Resin, with petroleum ether (60-90 °C) – ethyl acetate 5:1, detection by spraying with 5 % vanillin in sulfuric acid – ethanol 1:4 and heating at 100 °C until the zones are visible in daylight; (2) for Commiphora myrrha Engl./Commiphora molmol Engl. resin, with chloroform – ethyl acetate 80:1, detection as before; (3) for Daemonorops draco Bl., with chloroform – methanol 19:1, detection in daylight. Quantification of dracorhodin by HPLC.

J. Ethnopharmacol. 195, 324-333 (2017). HPTLC of quercetin (1), kaempferol (2), beta-sitosterol (3) and luteolin (4) in fresh flowers of Saraca asoca on silica gel with toluene – acetone – formic acid 10:4:1 for (1), cyclohexane – ethyl acetate – methanol – formic acid 12:9:1:1 for (2), toluene – methanol 8:1 for (3) and toluene – ethyl acetate – formic acid 15:15:4 for (4). Quantitative determination by absorbance measurement at 378 nm for (1), 365 nm for (2), 366 nm for (3) and 254 nm for (4). The hRF values for (1) to (4) were 29, 35, 42 and 45 respectively.

J. Planar Chromatogr. 29, 462-468 (2016). HPTLC of dantrolene sodium (1) and its process-related impurity 5-(4-nitrophenyl)-2-furaldehyde (2) on silica gel with chloroform – ethyl acetate – glacial acetic acid 1000:500:1. Quantitative determination by absorbance measurement at 380 nm. The hRF values for (1) and (2) were 22 and 83, respectively. Linearity ranged between 100-1500 ng/zone for (1) and 100-2000 ng/zone for (2). The intermediate precisions were below 0.3 % (n=3). The LODs and LOQs were 33 and 99 ng/zone for (1) and 32 and 96 ng/zone for (2), respectively. Average recoveries were 99.5 % for (1) and 100.2 % for (2).

J. Ethnopharmacol. 197, 242-249 (2017). Kanakasava is an Indian traditional Ayurvedic formulation containing Datura metel, Adhatoda vasica, Woodfordia fruticosa and Vitis vinifera extracts as major constituents. HPTLC of gallic acid (1) and ethyl gallate (2) on silica gel with toluene – ethyl acetate – formic acid – methanol 35:35:8:5. Quantitative determination by absorbance measurement at 292 nm. The hRF values for (1) and (2) were 43 and 48, respectively.

J. Planar Chromatogr. 30, 251-258 (2017). HPTLC of piperine in black pepper on silica gel with toluene ‒ ethyl acetate ‒ glacial acetic acid 14:6:1. Quantitative determination by absorbance measurement at 280 nm. The hRF value for piperine was 40. Linearity was between 100 and 900 ng/zone. LOD and LOQ were 20 and 60 ng/zone, respectively. The intermediate precision was <2 % (n=6). Recovery ranged from 99.2 to 100.2 %.

J. Ethnopharmacol. 197, 165-172 (2017). HPTLC of arjunolic acid (1), arjunetin (2), berberin (3), piperine (4), resveratrol (5) and withaferin-A (6) in Ayurvedic formulation on silica gel with ethyl acetate – toluene – formic acid – acetic acid 6:3:5:1 for (1), n-butanol – glacial acetic acid – water 12:3:4 for (2), toluene – ethyl acetate – diethylether 6:3:1 for (3), chloroform – ethyl acetate – formic acid 25:10:1 for (4) and chloroform – methanol 19:1 for (5) and (6). Detection of (1-4) by spraying with anisaldehyde sulfuric acid reagent. Quantitative determination by absorbance measurement at 690 nm for (1) and (2), 366 nm for (3), 330 nm for (4), 307 nm for (5) and 225 nm for (5) and (6). The hRF values for (1-6) were 67, 31, 58, 24, 32 and 37, respectively.

J. Chromatogr. A 1526, 151-156 (2017). Development of a rapid and cost-effective method for determining brominated flame retardants (BFR) such as tetrabromobisphenol A/S (TBBPA/S) and their derivatives in soils, including TBBPA, TBBPA bis(allyl ether) (TBBPA-BAE), TBBPA bis(2,3-dibromopropyl ether) (TBBPA-BDBPE), TBBPS bis(allyl ether) (TBBPS-BAE) and TBBPS bis(2,3-dibromopropyl ether) (TBBPS-BDBPE), based on TLC sample pre-treatment coupled with HPLC-DAD at UV 214 nm. The LODs and LOQs were between 0.023-0.087 μg/g dw and 0.076-0.29 μg/g dw, respectively. The recoveries were between 41-108 % and both % RSD of repeatability and intermediate precision were < 11 %. The method showed a good performance for analyzing natural soil samples collected from BFRs industrial park, suggesting its great application potential for monitoring environmental TBBPA/S and their derivatives.