Chinese J. of Med. Guide 10 (17), 98-99 (2012). Banlian Chongji electuary is herbal TCM preparation for the treatment of virus flu and upper respiratory infections. For quality control the indirubin and indigo contents are determined by TLC on silica gel with petroleum ether (60-90 °C) – ethyl acetate – chloroform 1:1:8. Detection in daylight, quantification by densitometry at 290 nm. The linearity was in the range of 0.1-0.5 µg/zone (n=5, r=0.9999) both for indirubin and indigo. The precision (%RSD, n=5) was 1.2 % for indirubin and 2.2 % for indigo. Recovery by standard addition was 95.5 % (%RSD=4.5 %, n=5) for indirubin, and 96.0 % (RSD%=2.9 %, n=5) for indigo.

J. Planar Chromatogr. 27, 291-296 (2014). HPTLC of phyllanthin (1), hypophyllanthin (2), and niranthin (3) from Phyllanthus amarus on silica gel with n-hexane - ethyl acetate - glacial acetic acid 30:10:1. Quantitative determination by absorbance measurement at 230 nm. The hRF values for (1) to (3) were 33, 41 and 46, respectively. Linearity was in the range of 200-1200 ng/zone for (1), 200-1000 ng/zone for (2) and 200-1000 ng/zone for (3). The intermediate/interday/intra-day precisions were below 2 % (n=6). The LOD and LOQ were 50 and 200 ng/zone for (1), 100 and 200 ng/zone for (2) and 100 and 200 ng/zone for (3), respectively. Average recoveries for (1) to (3) were 100.1 %, 98.8 % and 100.3 %, respectively.

J. Planar Chromatogr. 27, 132-139 (2014). HPTLC of levetiracetam on silica gel with toluene - ethyl acetate - methanol 2:1:1. Quantitative determination by absorbance measurement at 204 nm. The hRF value for levetiracetam was 50. Linearity was in the range of 1-1000 ng/zone. The intermediate/interday/intra-day precisions were below 1 % (n=6). The LOD and LOQ were 30 and 100 ng/zone, respectively. Recovery was between 99.2 and 100.9 %.

J. Liq. Chromatogr. Relat. Technol. 37, 1416-1426 (2014). HPTLC of lumefantrine (1) and artemether (2) on silica gel with toluene – ethyl acetate – acetic acid 4:15:5. Detection by dipping into a derivatization reagent (10 % each of methanolic concentrated sulfuric acid and anisaldehyde), followed by heating at 110 °C for 3 min. Quantitative determination by absorbance measurement at 519 nm. The hRF values for (1) and (2) were 55 and 70, respectively. Linearity was in the range of 60-360 ng/zone for (1) and 10-60 ng/zone for (2). The intermediate/interday/intra-day precisions were below 1 % (n=3). The LOD and LOQ were 7 and 26 ng/zone for (1) and 2 and 6 ng/zone for (2), respectively. Recoveries were between 97.6-101.3 % for both (1) and (2).

J. Planar Chromatogr. 27, 2942-2955 (2014). HPTLC of propafenone hydrochloride in tablets on silica gel with chloroform - methanol - acetic acid 79:20:1. Quantitative determination by absorbance measurement at 316 nm. The hRF value for propafenone hydrochloride was 64. Linearity was in the range of 100-3200 ng/zone. The intermediate/interday/intra-day precisions were below 2 % (n=3). The LODs and LOQs were 20 and 80 ng/zone, respectively. Recoveries were between 99.5 and 102.2 %.

J. Planar Chromatogr. 27, 460-465 (2014). HPTLC of (1) schisandrin, (2) gomisin B, (3) deoxyschisandrin and (4) gomisin N on silica gel with toluene – ethyl acetate – methanol 6:1:1. Quantitative determination by absorbance measurement at 225 nm. The hRF values of (1) to (4) were 20, 32, 61 and 72, respectively. Linearity was between 10 and 500 ng/zone for each substance. The intermediate intra-day and inter-day precision was below 3 % (n=5) for (1) to (4). The LOD and LOQ were 2.0 and 6.6 ng/zone for (1) and (2), and 2.5 and 8.2 for (3) and (4), respectively. Recovery for (1) to (4) were in the range of 97.2-102.3 %.

J. Liq. Chromatogr. Relat. Tech. 37, 2989-2999 (2014). HPTLC of neutral and polar lipid content of digestive gland-gonad complex of Biomphalaria glabrata on silica gel with petroleum ether - diethyl ether - glacial acetic acid 80:20:1 for neutral lipids, and chloroform - methanol - deionized water 65:25:4 for phospholipids. Detection of neutral lipids by spraying with 5 % ethanolic phosphoric acid, followed by heating at 115 ºC for 10 min. Phospholipids were detected by spraying with 10 % cupric sulfate in 8 % phosphoric acid, followed by heating at 140 ºC for 30 min. Quantitative determination by absorbance measurement at 610 nm for neutral lipids and 370 nm for polar lipids. The hRF values of neutral lipids were 19 for cholesterol, 38 for oleic acid and 57 for triolein, whereas for polar lipids were 46 for phosphatidylcholine and 66 for phosphatidylethanolamine.

J. Sep. Sci. 37, 2675-2681 (2014). HPTLC of the acidic catecholamine metabolites (1) 3,4-dihydroxymandelic acid, (2) epinephrine, (3) vanillylmandelic acid, (4) 3,4-dihydroxyphenylacetic acid and (5) homovanillic acid in human urine on RP-18 with citrate buffer (pH 3.0) - methanol - formic acid 48:2:5. Detection by spraying with 0.02 % 2,2-diphenyl-1-picrylhydrazyl radical solution in ethanol. Quantification by digital imaging processing. The hRF values of (1) to (5) were 84, 65, 59, 47 and 22, respectively. Linearity was in the range of 30-150 ng/zone for (1), (2) and (4), 90-450 ng/zone for (3) and 60-300 ng/zone for (5). The intermediate intra-day and inter-day precisions were below 3 % (n=3) for (1) to (5). The LOD and LOQ were 33 and 36 ng/zone for (1), 36 and 42 ng/zone for (2), 103 and 120 ng/zone for (3), 13 an 17 ng/zone for (4) and 45 and 54 ng/zone for (5), respectively. Recoveries for the compounds were between 94.6 and 105.7 %.