CBS 95, 9 (2005). HPTLC of glyphosate and AMPA derivatized in situ on the application position of the plate with FMOC, on silica gel with n-butanol - water - acetic acid 5:1:1 over 70 mm in an unsaturated twin trough chamber. After drying dipping in paraffin - toluene 1:1 for fluorescence enhancement. Quantitative determination by fluorescence measurement with mercury lamp at 265/M 360 nm. Linear calibration using peak height, LOD 0.5 ng absolute per substance zone for glyphosate-FMOC and 0.2 ng for AMPA-FMOC.

J. Planar Chromatogr. 18, 377-379 (2005). HPTLC of 2-azaanthraquinone from plant extracts on silica gel with toluene - ethyl acetate - methanol 40:9:1. Quantitative determination by absorbance measurement at 310 nm. Calibration was linear in the range of 10-100 µg/mL. The method was repeatable and precise with RSD between 0.98 and 1.59 % intra-day and between 3.41 and 5.56 % inter-day. Limits of detection and quantification were 3 and 6 µg/mL.

Hosp. Pharm. (Zhongguo Yiyuan Yaoxue Zazhi) 25 (9), 894-896 (2005). TLC of the extracts on silica gel plates with acetone - ethanol - hydrochloric acid 10:6:1. Detection by spraying with potassium iodobismuthate solution. Identification by comparison with the standard. Quantification by densitometry at 510 nm. Validation of the method by investigation of linearity range (2.2 µg - 10.8 µg, r = 0.9994); precision (RSD = 1.05 %, n = 5); reproducibility of five time assay towards the same sample (RSD = 0.31 %); and standard addition recovery (98.1 %, RSD = 2.15 %, n = 5). The results for five real life samples are given.

J. Chinese Trad. Patent Med. (Zhongchengyao) 27(5), 535-538 (2005). TLC of the extracts on silica gel with 1) benzene - ethyl acetate - formic acid 15:2:1; 2) n-hexane - ethyl acetate - formic acid 60:20:1; 3) chloroform - methanol - ammonia 40:10:1; 4) chloroform - methanol - water 13:7:2. Detection 1) under UV 365 nm; 2) by spraying with 3 % ninhydrin solution followed by heating at 105 ºC until the spots are visualized; 3) by spraying with 10 % H2SO4 solution in ethanol followed by heating until the spots are visualized. Identification by fingerprint technique. Quantification of emodin by densitometry at 445 nm. Validation of the method by investigation of its linearity range (0.1 µg - 1.0 µg, r = 0.998); precision (RSD = 1.05 % n = 6); reproducibility of six time assay towards the same sample (RSD = 1.24 %); and standard addition recovery (96.7 %, RSD = 1.75 %, n = 6). The results for three batches of real life sample are given.

Chinese J. Chromatogr. (Sepu) 23 (2), 217-218 (2005). TLC of the title compound on silica gel with chloroform - benzene 7:3. Detection 1) by spraying with 10 % H2SO4 in ethanol followed by heating at 120 ºC for 10 min; 2) by spraying with 10 % phosphomolybdic acid in ethanol followed by heating at 120 ºC for 10 min, then exposing to ammonia vapours. Quantification by comparison of the separated zone size with the standard. Optimization of the mobile phase by investigation of the influence of the composition of the developing solvent on Rf values. Optimization of the visualization condition by investigating the relationship between the sample dosage and the visualization results.

J. AOAC Int. 89, 1-7 (2006). TLC of masticadienonic and 3-hydroxymasticadienonic acid on silica gel with hexane - acetone - formic acid - acetic acid 30:10:1:1. Quantitation by determination of the absorption at 200 nm. Detection by dipping into anisaldehyde - sulfuric acid reagent for 1 sec and heating at 100 °C for 5 min. Limit of detection was 0.1-0.2 µg/mL.

Indian Drugs 42 (10), 671-674 (2005). HPTLC of desloratadine on silica gel with methanol - n-butanol - water - toluene - glacial acetic acid 20:30:10:20:1. Quantification in absorbance mode at 254 nm. The HPTLC system was quantitatively evaluated in terms of stability, precision, repeatability, specificity, accuracy and calibration, and was suitable for the analysis of desloratadine tablet dosage form. The linearity was in the range of 30-150 µg/mL with recovery of 98.8-102.0 %

J. Chinese Trad. Patent Med. (Zhongchengyao) 27 (6), 659-662 (2005). TLC of extracts of Yupingfeng oral liquid on silica gel with 1) cyclohexane - ethyl acetate 7:3. Detection by spraying with 5 % p-dimethylaminobenzaldehyde in H2SO4 - ethanol 1:9 and heating at 105 ºC until the spots are visualized. Identification by fingerprint techniques. Quantification of astragaloside by HPLC. The results for eight real life samples are given.