J. Planar Chromatogr. 21, 113-117 (2008). HPTLC of alpha-solanine and alpha-chaconine on silica gel with chloroform - methanol - water - aqueous ammonia 140:60:10:1. Detection by dipping in cerium(IV) sulfate derivatization reagent. Quantitative determination by absorbance measurement at 505 nm.

J. Planar Chromatogr. 21, 27-32 (2008). TLC of extracts of Stachys recta and harpagide, acetylharpagide, harpagoside, ajugoside, and aucubin on silica gel with chloroform - methanol - water 25:10:1. Visualization by spraying with Ehrlich’s reagent (1 % solution of dimethylaminobenzaldehyde in concentrated hydrochloric acid) followed by heating at 105 °C for 5 min. Densitometric evaluation at 540 nm.

Ind. J. Pharm. Sci. 69 (6), 834-836 (2007). HPTLC of olmesartan medoxomil and hydrochlorothiazide simultaneously in combined dosage forms on silica gel with acetonitrile - chloroform - acetic acid 14:4:1. Detection at 254 nm. Linearity was between 500 and 750 ng/spot for olmesartan medoxomil and 100 and 600 ng/spot for hydrochlorothiazide. The limit of detection and the limit of quantification for olmesartan medoxomil was 170 and 500 ng/spot respectively, and for hydrochlorothiazide 30 and 100 ng/spot, respectively. The proposed method can be used to determine the drug content of marketed tablet formulation.

fruits. Indian Drugs 45(11), 908-910 (2008). HPTLC of embelin in ethanolic extract of dried fruits of Embelia ribes Burm. on silica gel with ethyl acetate - methanol 9:1. Quantitative determination by absorbance measurement at 365 nm. The total content in Embelia ribes fruit was 0.034 %. The proposed HPTLC method provides a good resolution of embelin from other constituents present in the ethanolic extract of dried fruits of Embelia ribes Burm.

J. Liq. Chromatogr. Relat. Technol. 31, 1373-1385 (2008). TLC of cholic acid, glycocholic acid, glycolithocholic acid, deoxycholic acid, chenodeoxycholic acid, glycodeoxycholic acid, and lithocholic acid on silica gel with concentration zone, prewashed with methanol and dried for 24 h at room temperature, in a saturated chamber. Best separation of the bile acids was achieved with n-hexane - ethyl acetate - methanol - acetic acid 20:20:5:2. Detection by dipping for 15 s in sulfuric acid - methanol 1:19, followed by heating at 90 °C for 20 min provided better results than derivatization by spraying with 10 % phosphomolybdic acid in ethanol. Quantitative determination by absorbance measurement between 190 and 800 nm.

J. Liq. Chromatogr. Relat. Technol. 30, 2891-2902 (2007). HPTLC of L-glutamate and L-aspartate on silica gel by double elution with n-butanol - acetic acid - water 13:3:5 in a chamber saturated for 3 h. Detection by spraying with 0.2 % ninhydrin solution in acetone. Quantitative determination by absorbance measurement at 486 nm.

Food Chemistry 113, 640-644 (2009). HPTLC of curcumin (1), demethoxycurcumin (2), and bisdemethoxycurcumin (3) from the rhizomes of Curcuma longa on silica gel with chloroform – methanol 24:1. Quantitative determination by absorbance measurement at 425 nm. The hRf values of (1), (2), and (3) were 66, 48, and 30, respectively. Selectivity regarding matrix was given. Recovery was 98.7 % for (1), 96.3 % for (2), and 97.2 % for (3). The limit of detection for (1), (2), and (3) was 0.1 µg/spot. The linear regression equation was y=4447.26 + 61.993X for (1), y=1089.881 + 70.003X for (2), and y=2611.84 + 51.565X for (3).

60th Indian Pharmaceutical Congress PA-204, (2008). HPTLC of carvedilol on silica gel with methanol - ethyl acetate 13:7. The hRF was 49. Quantitative determination by absorbance measurement at 242 nm. Linearity was between 200-1000 ng/spot. The recovery was 98-102 %. The method was suitable for routine quality control of the drug in formulation.