Cumulative CAMAG Bibliography Service CCBS

Our CCBS database includes more than 11,000 abstracts of publications. Perform your own detailed search of TLC/HPTLC literature and find relevant information.

The Cumulative CAMAG Bibliography Service CCBS contains all abstracts of CBS issues beginning with CBS 51. The database is updated after the publication of every other CBS edition. Currently the Cumulative CAMAG Bibliography Service includes more than 11'000 abstracts of publications between 1983 and today. With the online version you can perform your own detailed TLC/HPTLC literature search:

  • Full text search: Enter a keyword, e.g. an author's name, a substance, a technique, a reagent or a term and see all related publications
  • Browse and search by CBS classification: Select one of the 38 CBS classification categories where you want to search by a keyword
  • Keyword register: select an initial character and browse associated keywords
  • Search by CBS edition: Select a CBS edition and find all related publications

Registered users can create a tailor made PDF of selected articles throughout CCBS search – simply use the cart icon on the right hand of each abstract to create your individual selection of abstracts. You can export your saved items to PDF by clicking the download icon.

Page
      108 099
      Simultaneous determination of pioglitazone, metformin, and glimepiride in pharmaceutical preparations using HPTLC method
      D. KALE, R. KAKDE* (*Department of Pharmaceutical Sciences, RTM Nagpur University, Amravati Road, Nagpur-440033, Maharashtra, India; drkakde@yahoo.com)

      J. Planar Chromatogr. 24, 331-336 (2011). HPTLC of pioglitazone (PIO), metformin (MET), and glimepiride (GLI) in pharmaceutical preparations on silica gel, prewashed with methanol, with acetonitrile - methanol - propanol - ammonium acetate solution 7:2:1:1 in a twin trough chamber saturated for 10 min. Quantitative determination by densitometry at 240 nm. The hRf value was 83, 21, and 89 for PIO, MET, and GLI, respectively. Linearity was in the concentration range of 300-1200 ng/band, 10-40 µg/band and 40-160 ng/band with correlation coefficients of 0.995, 0.996, and 0.998 for PIO, MET, and GLI, respectively. The LOD and LOQ was 57 and 171 ng for PIO, 6 µg and 18 µg for MET, and 12 and 36 ng for GLI. The %RSD for method and intermediate precision was below 2 %. The mean recovery (n = 5) was 98.2-99.5 % for PIO, 98.6-99.3 % for MET, and 98.7-99.7 % for GLI with %RSD between 0.4 and 1.3 %.

      Classification: 32a
      108 116
      Chemical fingerprinting of Turnera diffusa and closely related genera by high-performance thin-layer chromatography
      A.S. RAO*, J. SHAO, T.J. SMILLIE, I.A. KHAN (*National Center for Natural Products Research, Research Institute of Pharmaceutical Sciences, The University of Mississippi, University, MS 38677, USA)

      Planta Med. 74, 352-353 (2008). HPTLC of tetraphyllin, turneradiffusin, beta-arbutin, terniflorin, echinaticin, turneradin and methanolic extracts of Turnera diffusa on silica gel with ethyl acetate - acetic acid - water 190:10:1. Quantitative determination by densitometric absorbance measurement at 254 nm.

      Classification: 32e
      108 142
      Simultaneous analysis of hydrophilic and lipophilic compounds in Salvia miltiorrhiza by double-development HPTLC and scanning densitometry
      J. YANG (Yang Jing), L.-L. CHOI (Choi Lei-lei), D.-Q. LI (Li De-Qiang), F.-Q. YANG (Yang Feng-Qing), L.-J. ZENG (Zeng Ling-Jie), J. ZHAO (Zhao Jing), S.-P. LI (Li Shao-Ping)* (*State Key Laboratory for Quality Research in Chinese Medicine and Institute of Chinese Medical Sciences, University of Macau, Macao SAR, China; Lishaoping@hotmail.com)

      J. Planar Chromatogr. 24, 257-263 (2011). HPTLC of hydrophilic and lipophilic constituents of Salvia miltiorrhiza and standards protocatechuic acid and aldehyde, salvianolic acid A and B, dihydrotanshinone I, rosmarinic acid, caffeic acid, cryptotanshinone, tanshinone II A, tanshinone I, and miltirone on silica gel with dichloromethane - ethyl acetate - formic acid 11:12:5 for the first development and petroleum ether - ethyl acetate - cyclohexane 15:11:14 for the second development with chamber saturation for 30 min. The first mobile phase separated the hydrophilic constituents salvianolic acid B, salvianolic acid A, rosmarinic acid, caffeic acid, protocatechuic acid, and protocatechuic aldehyde. Detection under UV light at 254 and 365 nm. After documentation the plates were placed in a second chamber and development with the low polarity mobile phase which separated dihydrotanshinone I, cryptotanshinone, tanshinone I and II A, and miltirone. Detection under UV light at 254 and 365 nm. Quantitative determination by densitometry in absorbance mode at 260 or 290 nm. The linear range was between 0.1-0.3 and 0.7-8.3 µg/zone. Instrumental precision was less than 4 % (n = 6). Precision on one plate was below 5 % (n = 6) and on different plates below 14 %. Depending on the substance, the limits of detection and quantification were between 14-22 and 69-276 ng/zone, respectively. The repeatability (n = 6) was between 1.3-3.4 %. Some of the compounds had similar hRf values: for rosmarinic acid 44, for salvianolic acid 43, for caffeic acid 49, for protocatechuic acid 49, for dihydrotanshinone 65 and for cryptotanshinone 63. Additional detection by spraying with 5 % sulfuric acid in ethanol.

      Classification: 32e
      109 022
      Quantification of (-)-epicatechin and procyanidin B2 in chocolates
      V. GLAVNIK, B. SIMONOVSKA, Irena VOVK*, D. MUTAVDZIC PAVLOVIC, D. ASPERGER, S. BABIC (*National Institute of Chemistry, Laboratory for Food Chemistry, Hajdrihova 19, SI-1001 Lubljana, Slovenia; irena.vovk@ki.si)

      J. Planar Chromatogr. 24, 482-486 (2011). HPTLC of (-)-epicatechin and procyanidin B2 in chocolates on cellulose with n-propanol - water - acetic acid 20:80:1. Detection by immersion for 1 s in 4-dimethylaminocinnamaldehyde. Quantitative determination by densitometry at 655 nm. The samples contained 13 mg/100 g each of (-)-epicatechin and procyanidin B2 with a relative standard deviation of 5.8 and 4.2 % (n = 6), respectively. The calibration curves were polynomial in the range of 2-30 ng/zone for (-)-epicatechin and 4-60 ng/zone for procyanidin B2. LOD was 0.2 ng/zone (0.7 pmol) and 2 ng/zone (3.5 pmol) as well as LOQ was 0.4 ng/zone (1.4 pmol) and 4 ng/zone (7 pmol) for (-)-epicatechin and procyanidin, respectively.

      Classification: 8b
      109 042
      Enantiomeric thin-layer chromatographic assay of escitalopram in presence of „in-process impurities“
      Suzan M. SOLIMAN*, N. F. YOUSSEF (*National Organization of Drug Control and Research (NODCAR), 6-Abu Hazem street, Pyramide Ave. P. O. Box 29m Giza, Egypt; suzansoliman1961@hotmail. com)

      J. Planar Chromatogr. 24, 474-481 (2011). TLC of the active S-(+)-enantiomer escitalopram oxalate (ESC-OX), escitalopram cyanodiol, the R-enantiomer and escitalopram N-oxide impurities on silica gel (containing beta-cyclodextrin as chiral additive) with acetonitrile - 0.1 % acetic acid - water 10:1:6:2 with chamber saturation for 30 min. Using 3 mg urea per 100 cm2 of silica-coated plates as a chiral additive also achieves a good enantiomeric separation with acetonitrile - 1 % acetic acid - ethyl acetate - methanol - water 10:1:2:4:3. Detection at 254 nm. Quantitative determination by absorbance measurement of ESC-OX at 240 nm. The hRf values of ESC-OX, escitalopram cyanodiol, the R-enantiomer and escitalopram N-oxide were 75, 40, 31, and 23, respectively. The linearity was 0.25-10 mg/10 mL (r = 0.9991). Accuracy was 99.7 %. LOD and LOQ were 13 and 44 µg/mL for ESC-OX.

      Classification: 17c
      109 061
      A simple, rapid, and sensitive HPTLC method for the estimation of clarithromycin
      P. LOYA, P. D. HAMRAPURKAR* (*Principal K. M. Kundnani College of Pharmacy, Plot no. 23, Joy Building, Rambhau, Salgaonkar Marg, Cuffe parade, Colaba, Mumbai - 400 005, India; phamrapurkar@gmail.com)

      J. Planar Chromatogr. 24, 534-538 (2011). HPTLC of clarithromycin in plasma on silica gel (prewashed with methanol) with ethyl acetate - methanol - 15 % ammonium acetate (pH 10.6) 7:2:1 in a twin-trough chamber with saturation for 15 min. Detection by dipping into xanthydrol solution (10 % in methanol). Quantitative determination by densitometry in absorbance mode at 506 nm. The hRf was 62. The method was linear over the range of 0.1-3.0 µg/mL (r2 = 0.9974). The recovery (by standard addition) was over 85 %. The intra-day and inter-day precision (%RSD) of the assay was in the range of 0.8-4.6 %. The recovery was above 95 %.

      Classification: 28a
      109 083
      Validated HPTLC method for simultaneous analysis of alfuzosin hydrochloride and dutasteride in a pharmaceutical dosage form
      S.S. DESHMUKH, V.V. MUSALE, V.K. BHUSARI, S.R. DHANESHWAR* (*Department of Pharmaceutical Chemistry, Bharati Vidyapeeth University, Poona College of Pharmacy, Pune, Maharashtra, India 411038; sunildhaneshwar@gmail.com)

      J. Planar Chromatogr. 24, 218-221 (2011). HPTLC of alfuzosin hydrochloride (ALF) and dutasteride (DUTA) in the bulk drug and in a tablet formulation on silica gel with toluene - methanol - dichloromethane 6:1:1 + 1 drop triethylamine. Quantitative determination by densitometry at 247 nm. The hRf of ALF was 46 and of DUTA 65. Linearity was between 300-600 ng/band for ALF and 500-100 ng/band for DUTA. LOD and LOQ were 100 and 200 ng/band for ALF and 300 and 400 ng/band for DUTA. Precisions (%RSD) for repeatability of application were 1.8 and 1.5 % for ALF and 1.5 and 1.4 % for DUTA. The inter-day and intra-day precision (%RSD, n = 6) was 1.0 and 0.9 % for ALF and 1.7 and 0.8 % for DUTA, respectively. Recovery (by standard addition) was between 98.9-101.6 % for both compounds.

      Classification: 32a
      109 100
      Investigation of antiradical activity of plant material by thin-layer chromatography with image processing
      Marta OLECH, L. KOMSTA, Renata NOWAK*, L. CIESLA, Monika HAJNOS (*Department of Pharmaceutical Botany,Medical University of Lublin, 1 Chodzki Street, 20-093 Lublin, Poland, renata.nowak@umlub.pl)

      Food Chemistry 132, 549-553 (2012).. New HPTLC-based method to examine quantitatively the free radical scavenging activity of plant extracts. After chromatographic separation of polar compounds, and immersion of HPTLC plates in methanolic DPPH radical reagent, bleaching was observed and recorded using a photo camera and data analysis was carried out using an image processing software. The method is simple, fast and efficient for free-radical scavenging activity analysis of phytochemicals and crude plant extracts.

      Classification: 32e
Page