Chinese J. Trad. Pat. Med. (Zhongchengyao) 26 (9), App. 3-5 (2004). TLC on silica gel with 1) n-hexane - ethyl acetate 9:1; 2) chloroform - methanol 5:2; 3) n-hexane - ethyl acetate 3:1. Detection 1) under UV 365 nm; 2) by spraying with 10 % H2SO4 in ethanol and heating at 110 ºC; 3) by spraying with 5 % FeCl3 in ethanol. Identification by fingerprint technique. Quantification of icariine by HPLC.

J. Planar Chromatogr. 18, 23-27 (2005). HPTLC-AMD of fructo-oligosaccharides and inulin mixtures (sucrose, 1-kestose, nystose, and fructosylnystose) on diol phases at 55-65 % relative humidity in a twin-trough chamber with an acetonitrile - acetone - water polarity gradient. Detection by derivatization with 4-aminobenzoic acid reagent and quantitation by scanning at 366 nm.

J. Planar Chromatogr. 18, 28-33 (2005). HPTLC and TLC of four carbamate residues (pirimicarb, methomyl, carbofuran, carbaryl) in vegetables on silica gel (prewashed with chloroform - methanol 1:1 followed by drying at 110 °C for 30 min) with system I (two fold development with first toluene - acetone 4:1, and second dichloromethane - acetone 4:1), and system II (two fold development with first ethyl acetate - petroleum ether 3:2, and second chloroform - petroleum ether 9:1). Quantitative determination by densitometric scanning at 254 and 366 nm.

J. Pharm. Biomed. Anal. 37, 817-821 (2005). CO2 extracts of Harpagophytum procumbens root was evaluated by HPLC and HPTLC for harpagoside contents. HPTLC on silica gel with ethyl acetate - methanol - water 77:15:8 in saturated ADC chamber. Detection by dipping into anisaldehyde reagent followed by drying at 120 °C for 5 min. Quantitative determination by absorbance measurement at 509 nm. The linearity range was 0.04-0.40 mg/mL. The HPTLC method was less time consuming than HPLC, needing almost no sample pre-treatment. 15 different CO2-extracts of the plant were analysed.

Indian Drugs 42 (12), 802-804 (2005). HPTLC of Milagai Thailam on silica gel with toluene - acetone 7:3 with chamber saturation for 30 min. Quantitative determination by absorbance measurement at 254 nm. The hRf value of capsaicin was 17 and of piperine 27. Recovery rates were in the range of 99.3-99.5 %. The sample contained 0.97 mg/10 mL capsaicin and 0.6 mg/10 mL piperine. The method was found suitable for other herbal formulations containing capsaicin and piperine.

J. Chromatogr. A 1077 (2), 202-206 (2005). HPTLC of physcion, chrysophanol, emodin and chrysophanol glycoside in Rheum emodi on RP-18 with methanol - water - formic acid 80:19:1. Quantitative determination by absorbance measurement at 445 nm. The calibration curves were linear in the range of 20-100 ng for physcion, 80-400 ng for chrysophanol and emodin, and 200-1000 ng for chrysophanol glycoside. The method was found to be reproducible and convenient for quantitative analysis of anthraquinone derivatives in the methanolic extract of rhizomes of Rheum emodi collected from three different locations of Western Himalaya, India.

J. Planar Chromatogr. 18, 108-111 (2005). TLC of josamycin, sulfamethoxazole, carbamazepine, diclofenac, and iopromide after SPE on RP-18 with acetonitrile - water - acetic acid 5:5:2. Quantiative determination by absorbance measurement at 220 nm. Detectability of substances was lower than 1 µg (1.25 µg for sulfamethoxazole) per applied sample. The water was contaminated with all five drugs, concentrations found ranged from 0.017-1.314 µg / L water.

J. Liq. Chrom. & Rel. Technol. 26, 3453-3462 (2003). HPTLC of caffeine and acetaminophen on silica gel in a saturated twin-trough chamber with ethyl acetate - glacial acetic acid 19:1. Quantification at 254 nm. Diphenhydramine, pseudoephedrine, and acetaminophen were well separated from the caffeine zone. Precision (relative standard deviation) was 1.19 %; limit of detection was 0.2 µg for caffeine and 0.08 µg for acetaminophen; precision of duplicate samples (RSD) ranged from 0.95 to 7.56 %.