J. Liq. Chromatogr. Relat. Technol. 36, 197-212 (2013). HPTLC of lupeol (1) and stigmasterol (2) in the stems of Costus igneus on silica gel with n-hexane - ethyl acetate 4:1 for (1) and toluene - acetone - acetic acid 89:9:2 for (2). Detection by dipping in a solution of 0.5 mL p-anisaldehyde in 50 mL glacial acetic acid and 1 mL of 97 % sulfuric acid, followed by heating at 105 °C. Quantitative determination by absorbance measurement at 538 nm. The hRf values of (1) and (2) were 55 and 58, respectively. Linearity was in the range of 5-10 µg/zone for (1) and 1-6 µg/zone for (2). LOD and LOQ were 131 and 430 ng/zone for (1) and 80 and 212 ng/zone for (2). Intermediate precision was below 2.9 %. Average recovery (by standard addition) for (1) and (2) was 100.2 % and 99.9 %, respectively.

CBS 109, 13-15 (2012). HPTLC-AMD of tolyltriazoles and by-products after ozone treatment of water samples, on LiChrospher silica gel (pre-washed with 2-propanol and dried at 120 °C for 30 min) by automated multiple development (AMD) using a 22-step gradient from methanol - formic acid 200:1 over dichloromethane to n-hexane. Detection under UV 254 nm and by spraying with 2,4-dinitrophenylhydrazine reagent, evaluation under white light. Densitometric measurement at 190, 200, 220, 240, 260, 280, and 300 nm before derivatization and at 380, 400, and 420 nm after derivatization. Coupling of HPTLC with HPLC-QTOF/MS using the TLC-MS Interface offline with water - acetonitrile 1:1 with 5 mmol ammonium acetate as extraction solvent and a flow rate of 0.2 mL/min.

J Vet Sci Med Diagn 2(3), 1-3 (2013). HPTLC of neutral lipids on silica gel (HLF plates with 19 scored channels of 9 mm width) prewashed with dichloromethane - methanol 1:1 and heated for 30 min at 120 °C, with petroleum ether - diethyl ether - glacial acetic acid 80:20:1 with chamber saturation. Detection of dark zones on a yellow background after spraying with 5 % ethanolic phosphomolybdic acid reagent. Quantification of triacylglycerols by absorption measurement at 610 nm with polynomial regression via peak area.

J. Planar Chromatogr. 26, 475-479 (2013). HPTLC of beta-amyrin in the aerial parts of Maytenus obscura and Maytenus parviflora on silica gel with hexane - ethyl acetate 3:1. Detection by spraying with p-anisaldehyde, followed by heating at 105 ºC for 10 min. Quantification by absorbance measurement at 550 nm. The hRf of beta-amyrin was 38. Linearity was in the range of 10-100 ng/zone. LOD and LOQ were 9 and 27 ng/zone. Recovery was in the range of 99.3-99.7 %. Intermediate/interday/intra-day precision was below 2 % (n=6).

J. Planar Chromatogr. 26, 370-374 (2013). HPTLC of ciprofloxacin in tablets on silica gel with acetone - water - ammonia 30:3:5. Quantification by absorbance measurement at 280 nm. The hRf of ciprofloxacin was 27. Linearity was in the range of 250-600 ng/zone. Recovery was in the range of 96-101 %. Intermediate/interday/intra-day precision was below 2 %.

J. Planar Chromatogr. 26, 312-315 (2013). HPTLC of L-dopa (1) and rutin (2) in a polyherbal formulation on silica gel with butan-1-ol - acetic acid - water 4:1:1 for (1) and ethyl acetate - butan-1-ol - formic acid - water 5:3:1:1 for (2). Quantification by absorbance measurement at 288 nm for (1) and 254 nm for (2). The hRf values for compounds (1) and (2) were 45 and 50, respectively. Linearity was in the range of 0.4-1.2 µg/zone for (1) and 0.4-0.9 µg/zone for (2). LOD and LOQ were 64 and 159 ng/zone for (1) and 76 and 228 ng/zone for (2), respectively. Average recoveries were 99.6 % for (1) and 100.1 % for (2). Intermediate/interday/intra-day precision was below 5 % (n=8).

J. Planar Chromatogr. 27, 204-209 (2014). HPTLC of naringin in the leaves and stems of Rumex vesicarius on silica gel with ethyl acetate - glacial acetic acid - methanol - water 30:10:5:1. Quantitative determination by absorbance measurement at 275 nm. The hRF value for naringin was 46. Linearity was in the range of 100-1000 ng/zone. The intermediate/interday/intra-day precisions were below 2 % (n=6). The LOD and LOQ were 37 and 111 ng/zone, respectively. The average recovery was 99.5 %.

J. Liq. Chromatogr. Relat. Technol. 37, 2258-2274 (2014). HPTLC of 14-deoxy-11,12-didehydroandrographolide (1), andrographolide (2), and neoandrographolide (3) on silica gel with chloroform – methanol 7:1. Quantitative determination by absorbance measurement at 263 nm for (1), 235 nm for (2) and 212 nm for (3). The hRF values for (1) to (3) were 75, 42 and 35, respectively. Linearity was in the range of 200-900 ng/zone for (1) to (3). The intermediate/interday/intra-day precisions were below 2 % (n=3). Average recovery was 96.8 % for (1), 95.4 % for (2) and 87.6 % for (3).