Yunan J. of Chinese Trad. Med. & Pharm. 31(10), 58-60 (2010). TLC of extracts of Cishushi suppository: 1) for Panax notoginseng, on silica gel with chloroform – methanol – water 14:6:1, detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 ºC; 2) for Fructus Cnidii, on silica gel with petroleum ether (60-90 °C) – ethyl acetate 7:3, detection under UV 366 nm; 3) for Borneolum Syntheticum, on silica gel with toluene – ethyl acetate 19:1, detection by spraying with 5 % vanillin-sulfuric acid reagent and heating at 105 °C; 4) for Fructus Sophorae, on silica gel with chloroform – methanol – water – formic acid 700:300:50:1, detection by spraying with 1 % AlCl3 in ethanol, heating and evaluation under UV 366 nm.

J. Planar Chromatogr. 24, 357-359 (2011). TLC of benzo[a]pyrene on RP-18, RP-8, and RP-2 with acetone - ethyl acetate 7:3 in an unsaturated twin-trough chamber. Detection by dipping for 1 s into a solution of 250 mg bis(2,4-dinitrophenyl)oxalate in 40 mL of n-butyl acetate and 5 mL of hydrogen peroxide (upper phase). For videodensitometric evaluation the sheets were measured for 2 min and monitored over a period of 2 h. The reaction energy is transferred to the fluorescent compound (benzo[a]pyrene) which emits light while relaxing from its excited state. The advantage of this chemiluminescence reaction performed on a TLC plate is the improvement of the detection limit by extending the measuring time. The detection limit is 50 pg benzo[a]pyrene which is identical to that achievable in chemiluminescence HPLC. The calibration curve for benzo[a]pyrene was linear in the range of 50-8000 pg. The new procedure overcomes the problem of uneven illumination with CCD cameras.

J. Planar Chromatogr. 24, 206-210 (2011). HPTLC of free sterols, free fatty acids, triacylglycerols, methyl esters, and steryl esters in larvae samples on silica gel with concentration zone, with petroleum ether - diethyl ether - glacial acetic acid 80:20:1 in a twin-trough chamber saturated for 20 min at 21 +/- 1 °C and a relative humidity of 25 %. Detection by spraying with 5 % ethanolic phosphomolybdic acid solution. Quantitative determination by densitometry at 610 nm.

J. Planar Chromatogr. 24, 487-490 (2011). HPTLC of vitamin D3 in fish oil after saponification and purification on silica gel with chloroform - diethyl ether 9:1 with chamber saturation for 20 min. Quantitative determination at 280 nm. Linearity was between 200 and 1000 ng/band. The LOD and LOQ were 29 and 232 ng/band, respectively. The repeatability (RSD) was 4.6 %; the %RSD for intermediate precision was 0.9; the %RSD for intra-day precision (n = 6) was between 2.9-5.9 % and the %RSD for inter-day precision (n = 6) was between 3.2-6.4 %. Recovery was 97.6 - 104.2 % with a %RSD of 3.9 - 4.8 %.

Tianjin J. of Trad. Chinese Med. & Pharm. 28 (2), 164-166 (2011). TLC of the extracts of the traditional Mongolian medicine on silica gel 1) for artificial cow-bezoar, with isooctane - ethyl acetate - glacial acetic acid 6:3:2, detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 °C until the zones were detected under UV 365 nm, identification by comparison with the fingerprint of the individual drug components and cholic acid as reference; 2) for Coptidis rhizoma, with benzene - ethyl acetate - isopropanol - methanol - water 20:10:5:5:1, detection under UV 365 nm after exposure to ammonia vapour, identification by comparison of the fingerprint with the individual drug components and berberine hydrochloride as reference; 3) for light yellow Sophora root, with ethyl acetate - propanone - benzene - ammonia water 20:15:10:1, detection by spraying with 5 % potassium iodobismuthate solution, identification by comparison of the fingerprint with the individual drug components and with matrine as reference.

J. Planar Chromatogr. 24, 232-235 (2011). HPTLC of darunavir ethanolate in tablets on silica gel, prewashed with methanol, with toluene - ethyl acetate - methanol 7:2:1 in a twin-trough chamber lined with filter paper and saturated with mobile phase for 30 min at room temperature (25 +/- 2 °C). Quantitative determination by densitometry in absorbance mode at 267 nm. The hRf of darunavir ethanolate was 47. Linearity was between 250 and 1750 ng/band with r = 0.9994. The limits of detection and quantification were 15 and 46 ng/band, respectively. The intra-day and inter-day precision was (%RSD, n = 6) between 0.5-0.9 % and 1.1-1.3 %. Recovery (n = 6) was 99.3-101.2 %.

Planta Med. 75, 12-17 (2009). HPTLC of swertiamarin and the ethyl acetate extract of Enicostemma axillare on silica gel with ethyl acetate - butanol 1:1 in a twin trough chamber. Quantitative determination by densitometric absorbance measurement at 254 nm.

J. Planar Chromatogr. 23, 369-372 (2010). HPTLC of venlafaxine hydrochloride on silica gel with concentration zone, prewashed with methanol, with toluene - methanol 17:7 in a twin-trough chamber saturated for 10 min at 25 +/- 2 °C. Quantitative determination by absorbance measurement at 228 nm. The hRf value was 19. The validated calibration range was 400-2000 ng/band (r = 0.999). Recovery was 98.8-100.3 %. The intra-day precision as %RSD was 0.3-0.6 % and the inter-day precision 0.1-0.3 %. The LOD and LOQ were 97 ng and 294 ng, respectively.